Effects of silver nanoparticles on neonatal testis development in mice

Xi-Feng Zhang,1,2 Sangiliyandi Gurunathan,1 Jin-Hoi Kim1 1Department of Animal Biotechnology, Konkuk University, Seoul, Republic of Korea; 2College of Biological and Pharmaceutical Engineering, Wuhan Polytechnic University, Wuhan, People’s Republic of China Background: Metal nanoparticle...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Zhang XF, Gurunathan S, Kim JH
Formato:	article
Lenguaje:	EN
Publicado:	Dove Medical Press 2015
Materias:	Medicine (General) R5-920
Acceso en línea:	https://doaj.org/article/8c1165723a764e31ba7951aae2a82a36
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:8c1165723a764e31ba7951aae2a82a36
record_format	dspace
spelling	oai:doaj.org-article:8c1165723a764e31ba7951aae2a82a362021-12-02T07:13:41ZEffects of silver nanoparticles on neonatal testis development in mice1178-2013https://doaj.org/article/8c1165723a764e31ba7951aae2a82a362015-10-01T00:00:00Zhttps://www.dovepress.com/effects-of-silver-nanoparticles-on-neonatal-testis-development-in-mice-peer-reviewed-article-IJNhttps://doaj.org/toc/1178-2013Xi-Feng Zhang,1,2 Sangiliyandi Gurunathan,1 Jin-Hoi Kim1 1Department of Animal Biotechnology, Konkuk University, Seoul, Republic of Korea; 2College of Biological and Pharmaceutical Engineering, Wuhan Polytechnic University, Wuhan, People’s Republic of China Background: Metal nanoparticles (MNPs) play an important role in consumer products. An increasing use of MNPs has raised concerns about potential risks for human health. Therefore, in vivo tests of MNPs are urgently required. Using mice as a model animal, the aim of the present study was designed to investigate the effect of biologically synthesized silver nanoparticles (AgNPs) on spermatogenesis in neonatal mice. Methods: AgNPs were synthesized using Bacillus funiculus. The prepared nanoparticles were characterized using various analytical techniques such as UV–visible spectroscopy, X-ray diffraction, Fourier transform-infrared spectroscopy, and transmission electron microscopy. The prepared AgNPs were used to investigate testis development in neonatal mice. Institute of Cancer Research neonatal male mice were used in all experiments and were treated with different doses (0, 1, and 5 mg/kg) of AgNPs five times (interval of 3 days from postnatal day [PND] 8–21) by abdominal subcutaneous injection. Results: The results showed that the sperm abnormalities such as quality and quantity were significantly increased by the synthesized AgNPs. The diameter of the convoluted tubules shrank significantly in mice treated with AgNPs on PND28 and PND42. The results of reverse transcription-quantitative polymerase chain reaction indicated that the E1f1ay, Gsta4, and Fdx1 genes were up-regulated, and the Amh, Cx43, and Claudin-11 genes were down-regulated in response to AgNPs exposure on PND28; however, these genes recovered at PND60. AgNPs had no effect on the recombination levels of chromosomes in germ cells. Conclusion: These results demonstrated the adverse effects of AgNPs on the male reproductive tract, particularly spermatogenesis and the quality of sperm. This study suggests that the development of nanomaterials should be safer and non-toxic to the living organisms and the potential reprotoxicity of AgNPs should be investigated more carefully. Keywords: silver nanoparticles, male mouse, sperm, gene expression, testis developmentZhang XFGurunathan SKim JH,Dove Medical PressarticleMedicine (General)R5-920ENInternational Journal of Nanomedicine, Vol 2015, Iss default, Pp 6243-6256 (2015)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine (General) R5-920
spellingShingle	Medicine (General) R5-920 Zhang XF Gurunathan S Kim JH, Effects of silver nanoparticles on neonatal testis development in mice
description	Xi-Feng Zhang,1,2 Sangiliyandi Gurunathan,1 Jin-Hoi Kim1 1Department of Animal Biotechnology, Konkuk University, Seoul, Republic of Korea; 2College of Biological and Pharmaceutical Engineering, Wuhan Polytechnic University, Wuhan, People’s Republic of China Background: Metal nanoparticles (MNPs) play an important role in consumer products. An increasing use of MNPs has raised concerns about potential risks for human health. Therefore, in vivo tests of MNPs are urgently required. Using mice as a model animal, the aim of the present study was designed to investigate the effect of biologically synthesized silver nanoparticles (AgNPs) on spermatogenesis in neonatal mice. Methods: AgNPs were synthesized using Bacillus funiculus. The prepared nanoparticles were characterized using various analytical techniques such as UV–visible spectroscopy, X-ray diffraction, Fourier transform-infrared spectroscopy, and transmission electron microscopy. The prepared AgNPs were used to investigate testis development in neonatal mice. Institute of Cancer Research neonatal male mice were used in all experiments and were treated with different doses (0, 1, and 5 mg/kg) of AgNPs five times (interval of 3 days from postnatal day [PND] 8–21) by abdominal subcutaneous injection. Results: The results showed that the sperm abnormalities such as quality and quantity were significantly increased by the synthesized AgNPs. The diameter of the convoluted tubules shrank significantly in mice treated with AgNPs on PND28 and PND42. The results of reverse transcription-quantitative polymerase chain reaction indicated that the E1f1ay, Gsta4, and Fdx1 genes were up-regulated, and the Amh, Cx43, and Claudin-11 genes were down-regulated in response to AgNPs exposure on PND28; however, these genes recovered at PND60. AgNPs had no effect on the recombination levels of chromosomes in germ cells. Conclusion: These results demonstrated the adverse effects of AgNPs on the male reproductive tract, particularly spermatogenesis and the quality of sperm. This study suggests that the development of nanomaterials should be safer and non-toxic to the living organisms and the potential reprotoxicity of AgNPs should be investigated more carefully. Keywords: silver nanoparticles, male mouse, sperm, gene expression, testis development
format	article
author	Zhang XF Gurunathan S Kim JH,
author_facet	Zhang XF Gurunathan S Kim JH,
author_sort	Zhang XF
title	Effects of silver nanoparticles on neonatal testis development in mice
title_short	Effects of silver nanoparticles on neonatal testis development in mice
title_full	Effects of silver nanoparticles on neonatal testis development in mice
title_fullStr	Effects of silver nanoparticles on neonatal testis development in mice
title_full_unstemmed	Effects of silver nanoparticles on neonatal testis development in mice
title_sort	effects of silver nanoparticles on neonatal testis development in mice
publisher	Dove Medical Press
publishDate	2015
url	https://doaj.org/article/8c1165723a764e31ba7951aae2a82a36
work_keys_str_mv	AT zhangxf effectsofsilvernanoparticlesonneonataltestisdevelopmentinmice AT gurunathans effectsofsilvernanoparticlesonneonataltestisdevelopmentinmice AT kimjh effectsofsilvernanoparticlesonneonataltestisdevelopmentinmice
_version_	1718399587404742656

Effects of silver nanoparticles on neonatal testis development in mice

Ejemplares similares