Physical mechanisms driving the reversible aggregation of Staphylococcus aureus and response to antimicrobials

Abstract Formation of non-sessile, auto-aggregated cells of Staphylococcus aureus contributes to surface colonization and biofilm formation, hence play a major role in the early establishment of infection and in tolerance to antimicrobials. Understanding the mechanism of aggregation and the impact o...

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Autores principales:	Céline Burel, Rémi Dreyfus, Laura Purevdorj-Gage
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/8c1c8fe0026348b0827e6fe1512e169d
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spelling	oai:doaj.org-article:8c1c8fe0026348b0827e6fe1512e169d2021-12-02T17:57:15ZPhysical mechanisms driving the reversible aggregation of Staphylococcus aureus and response to antimicrobials10.1038/s41598-021-94457-12045-2322https://doaj.org/article/8c1c8fe0026348b0827e6fe1512e169d2021-07-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-94457-1https://doaj.org/toc/2045-2322Abstract Formation of non-sessile, auto-aggregated cells of Staphylococcus aureus contributes to surface colonization and biofilm formation, hence play a major role in the early establishment of infection and in tolerance to antimicrobials. Understanding the mechanism of aggregation and the impact of aggregation on the activity of antimicrobials is crucial in achieving a better control of this important pathogen. Previously linked to biological phenomena, physical interactions leading to S. aureus cellular aggregation and its protective features against antimicrobials remain unraveled. Herein, in-vitro experiments coupled with XDLVO simulations reveal that suspensions of S. aureus cells exhibit rapid, reversible aggregation (> 70%) in part controlled by the interplay between cellular hydrophobicity, surface potential and extracellular proteins. Changing pH and salt concentration in the extracellular media modulated the cellular surface potential but not the hydrophobicity which remained consistent despite these variations. A decrease in net cellular negative surface potential achieved by decreasing pH or increasing salt concentrations, caused attractive forces such as the hydrophobic and cell–protein interactions to prevail, favoring immediate aggregation. The aggregation significantly increased the tolerance of S. aureus cells to quaternary ammonium compounds (QAC). The well-dispersed cell population was completely inactivated within 30 s whereas its aggregated counterpart required more than 10 min.Céline BurelRémi DreyfusLaura Purevdorj-GageNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-9 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Céline Burel Rémi Dreyfus Laura Purevdorj-Gage Physical mechanisms driving the reversible aggregation of Staphylococcus aureus and response to antimicrobials
description	Abstract Formation of non-sessile, auto-aggregated cells of Staphylococcus aureus contributes to surface colonization and biofilm formation, hence play a major role in the early establishment of infection and in tolerance to antimicrobials. Understanding the mechanism of aggregation and the impact of aggregation on the activity of antimicrobials is crucial in achieving a better control of this important pathogen. Previously linked to biological phenomena, physical interactions leading to S. aureus cellular aggregation and its protective features against antimicrobials remain unraveled. Herein, in-vitro experiments coupled with XDLVO simulations reveal that suspensions of S. aureus cells exhibit rapid, reversible aggregation (> 70%) in part controlled by the interplay between cellular hydrophobicity, surface potential and extracellular proteins. Changing pH and salt concentration in the extracellular media modulated the cellular surface potential but not the hydrophobicity which remained consistent despite these variations. A decrease in net cellular negative surface potential achieved by decreasing pH or increasing salt concentrations, caused attractive forces such as the hydrophobic and cell–protein interactions to prevail, favoring immediate aggregation. The aggregation significantly increased the tolerance of S. aureus cells to quaternary ammonium compounds (QAC). The well-dispersed cell population was completely inactivated within 30 s whereas its aggregated counterpart required more than 10 min.
format	article
author	Céline Burel Rémi Dreyfus Laura Purevdorj-Gage
author_facet	Céline Burel Rémi Dreyfus Laura Purevdorj-Gage
author_sort	Céline Burel
title	Physical mechanisms driving the reversible aggregation of Staphylococcus aureus and response to antimicrobials
title_short	Physical mechanisms driving the reversible aggregation of Staphylococcus aureus and response to antimicrobials
title_full	Physical mechanisms driving the reversible aggregation of Staphylococcus aureus and response to antimicrobials
title_fullStr	Physical mechanisms driving the reversible aggregation of Staphylococcus aureus and response to antimicrobials
title_full_unstemmed	Physical mechanisms driving the reversible aggregation of Staphylococcus aureus and response to antimicrobials
title_sort	physical mechanisms driving the reversible aggregation of staphylococcus aureus and response to antimicrobials
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/8c1c8fe0026348b0827e6fe1512e169d
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Physical mechanisms driving the reversible aggregation of Staphylococcus aureus and response to antimicrobials

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