Combining low-density cell culture, single-cell tracking, and patch-clamp to monitor the behavior of postnatal murine cerebellar neural stem cells

Summary: Low-density cell culture of the postnatal cerebellum, combined with live imaging and single-cell tracking, allows the behavior of postnatal cerebellar neural stem cells (NSCs) and their progeny to be monitored. Cultured cerebellar NSCs maintain their neurogenic nature giving rise, in the sa...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Aida Menéndez-Méndez, Lucía Paniagua-Herranz, Luis A. Olivos-Oré, Rosa Gómez-Villafuertes, Raquel Pérez-Sen, Esmerilda G. Delicado, Antonio R. Artalejo, Felipe Ortega
Formato:	article
Lenguaje:	EN
Publicado:	Elsevier 2021
Materias:	Cell Biology Cell culture Single Cell Developmental biology Microscopy Neuroscience Science (General) Q1-390
Acceso en línea:	https://doaj.org/article/8c1d8c0273374a7580a20f18f218acef
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:8c1d8c0273374a7580a20f18f218acef
record_format	dspace
spelling	oai:doaj.org-article:8c1d8c0273374a7580a20f18f218acef2021-11-20T05:13:52ZCombining low-density cell culture, single-cell tracking, and patch-clamp to monitor the behavior of postnatal murine cerebellar neural stem cells2666-166710.1016/j.xpro.2021.100964https://doaj.org/article/8c1d8c0273374a7580a20f18f218acef2021-12-01T00:00:00Zhttp://www.sciencedirect.com/science/article/pii/S2666166721006705https://doaj.org/toc/2666-1667Summary: Low-density cell culture of the postnatal cerebellum, combined with live imaging and single-cell tracking, allows the behavior of postnatal cerebellar neural stem cells (NSCs) and their progeny to be monitored. Cultured cerebellar NSCs maintain their neurogenic nature giving rise, in the same relative proportions that exist in vivo, to the neuronal progeny generated by the three postnatal cerebellar neurogenic niches. This protocol describes the identification of the nature of the progeny through both post-imaging immunocytochemistry and patch-clamp recordings.For complete details on the use and execution of this protocol, please refer to Paniagua-Herranz et al. (2020b).Aida Menéndez-MéndezLucía Paniagua-HerranzLuis A. Olivos-OréRosa Gómez-VillafuertesRaquel Pérez-SenEsmerilda G. DelicadoAntonio R. ArtalejoFelipe OrtegaElsevierarticleCell BiologyCell cultureSingle CellDevelopmental biologyMicroscopyNeuroscienceScience (General)Q1-390ENSTAR Protocols, Vol 2, Iss 4, Pp 100964- (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Cell Biology Cell culture Single Cell Developmental biology Microscopy Neuroscience Science (General) Q1-390
spellingShingle	Cell Biology Cell culture Single Cell Developmental biology Microscopy Neuroscience Science (General) Q1-390 Aida Menéndez-Méndez Lucía Paniagua-Herranz Luis A. Olivos-Oré Rosa Gómez-Villafuertes Raquel Pérez-Sen Esmerilda G. Delicado Antonio R. Artalejo Felipe Ortega Combining low-density cell culture, single-cell tracking, and patch-clamp to monitor the behavior of postnatal murine cerebellar neural stem cells
description	Summary: Low-density cell culture of the postnatal cerebellum, combined with live imaging and single-cell tracking, allows the behavior of postnatal cerebellar neural stem cells (NSCs) and their progeny to be monitored. Cultured cerebellar NSCs maintain their neurogenic nature giving rise, in the same relative proportions that exist in vivo, to the neuronal progeny generated by the three postnatal cerebellar neurogenic niches. This protocol describes the identification of the nature of the progeny through both post-imaging immunocytochemistry and patch-clamp recordings.For complete details on the use and execution of this protocol, please refer to Paniagua-Herranz et al. (2020b).
format	article
author	Aida Menéndez-Méndez Lucía Paniagua-Herranz Luis A. Olivos-Oré Rosa Gómez-Villafuertes Raquel Pérez-Sen Esmerilda G. Delicado Antonio R. Artalejo Felipe Ortega
author_facet	Aida Menéndez-Méndez Lucía Paniagua-Herranz Luis A. Olivos-Oré Rosa Gómez-Villafuertes Raquel Pérez-Sen Esmerilda G. Delicado Antonio R. Artalejo Felipe Ortega
author_sort	Aida Menéndez-Méndez
title	Combining low-density cell culture, single-cell tracking, and patch-clamp to monitor the behavior of postnatal murine cerebellar neural stem cells
title_short	Combining low-density cell culture, single-cell tracking, and patch-clamp to monitor the behavior of postnatal murine cerebellar neural stem cells
title_full	Combining low-density cell culture, single-cell tracking, and patch-clamp to monitor the behavior of postnatal murine cerebellar neural stem cells
title_fullStr	Combining low-density cell culture, single-cell tracking, and patch-clamp to monitor the behavior of postnatal murine cerebellar neural stem cells
title_full_unstemmed	Combining low-density cell culture, single-cell tracking, and patch-clamp to monitor the behavior of postnatal murine cerebellar neural stem cells
title_sort	combining low-density cell culture, single-cell tracking, and patch-clamp to monitor the behavior of postnatal murine cerebellar neural stem cells
publisher	Elsevier
publishDate	2021
url	https://doaj.org/article/8c1d8c0273374a7580a20f18f218acef
work_keys_str_mv	AT aidamenendezmendez combininglowdensitycellculturesinglecelltrackingandpatchclamptomonitorthebehaviorofpostnatalmurinecerebellarneuralstemcells AT luciapaniaguaherranz combininglowdensitycellculturesinglecelltrackingandpatchclamptomonitorthebehaviorofpostnatalmurinecerebellarneuralstemcells AT luisaolivosore combininglowdensitycellculturesinglecelltrackingandpatchclamptomonitorthebehaviorofpostnatalmurinecerebellarneuralstemcells AT rosagomezvillafuertes combininglowdensitycellculturesinglecelltrackingandpatchclamptomonitorthebehaviorofpostnatalmurinecerebellarneuralstemcells AT raquelperezsen combininglowdensitycellculturesinglecelltrackingandpatchclamptomonitorthebehaviorofpostnatalmurinecerebellarneuralstemcells AT esmerildagdelicado combininglowdensitycellculturesinglecelltrackingandpatchclamptomonitorthebehaviorofpostnatalmurinecerebellarneuralstemcells AT antoniorartalejo combininglowdensitycellculturesinglecelltrackingandpatchclamptomonitorthebehaviorofpostnatalmurinecerebellarneuralstemcells AT felipeortega combininglowdensitycellculturesinglecelltrackingandpatchclamptomonitorthebehaviorofpostnatalmurinecerebellarneuralstemcells
_version_	1718419494683017216

Combining low-density cell culture, single-cell tracking, and patch-clamp to monitor the behavior of postnatal murine cerebellar neural stem cells

Ejemplares similares