Proinflammatory cytokines and response to molds in mononuclear cells of patients with Meniere disease

Abstract Epidemiological studies have found a higher prevalence of allergic symptoms and positive prick tests in patients with Meniere’s disease (MD); however the effect of allergenic extracts in MD has not been established. Thus, this study aims to determine the effect of Aspergillus and Penicilliu...

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Autores principales:	Lidia Frejo, Alvaro Gallego-Martinez, Teresa Requena, Eduardo Martin-Sanz, Juan Carlos Amor-Dorado, Andres Soto-Varela, Sofia Santos-Perez, Juan Manuel Espinosa-Sanchez, Angel Batuecas-Caletrio, Ismael Aran, Jesus Fraile, Marcos Rossi-Izquierdo, Jose Antonio Lopez-Escamez
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2018
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/8c2219bd572b4c5098c96c4491eaca20
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spelling	oai:doaj.org-article:8c2219bd572b4c5098c96c4491eaca202021-12-02T15:08:06ZProinflammatory cytokines and response to molds in mononuclear cells of patients with Meniere disease10.1038/s41598-018-23911-42045-2322https://doaj.org/article/8c2219bd572b4c5098c96c4491eaca202018-04-01T00:00:00Zhttps://doi.org/10.1038/s41598-018-23911-4https://doaj.org/toc/2045-2322Abstract Epidemiological studies have found a higher prevalence of allergic symptoms and positive prick tests in patients with Meniere’s disease (MD); however the effect of allergenic extracts in MD has not been established. Thus, this study aims to determine the effect of Aspergillus and Penicillium stimulation in cytokine release and gene expression profile in MD. Patients with MD showed higher basal levels of IL-1β, IL-1RA, IL-6 and TNF-α when compared to healthy controls. We observed that IL-1β levels had a bimodal distribution suggesting two different subgroups of patients, with low and high basal levels of cytokines. Gene expression profile in peripheral blood mononuclear cells (PBMC) showed significant differences in patients with high and low basal levels of IL-1β. We found that both mold extracts triggered a significant release of TNF-α in MD patients, which were not found in controls. Moreover, after mold stimulation, MD patients showed a different gene expression profile in PBMC, according to the basal levels of IL-1β. The results indicate that a subset of MD patients have higher basal levels of proinflammatory cytokines and the exposure to Aspergillus and Penicillium extracts may trigger additional TNF-α release and contribute to exacerbate inflammation.Lidia FrejoAlvaro Gallego-MartinezTeresa RequenaEduardo Martin-SanzJuan Carlos Amor-DoradoAndres Soto-VarelaSofia Santos-PerezJuan Manuel Espinosa-SanchezAngel Batuecas-CaletrioIsmael AranJesus FraileMarcos Rossi-IzquierdoJose Antonio Lopez-EscamezNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 8, Iss 1, Pp 1-11 (2018)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Lidia Frejo Alvaro Gallego-Martinez Teresa Requena Eduardo Martin-Sanz Juan Carlos Amor-Dorado Andres Soto-Varela Sofia Santos-Perez Juan Manuel Espinosa-Sanchez Angel Batuecas-Caletrio Ismael Aran Jesus Fraile Marcos Rossi-Izquierdo Jose Antonio Lopez-Escamez Proinflammatory cytokines and response to molds in mononuclear cells of patients with Meniere disease
description	Abstract Epidemiological studies have found a higher prevalence of allergic symptoms and positive prick tests in patients with Meniere’s disease (MD); however the effect of allergenic extracts in MD has not been established. Thus, this study aims to determine the effect of Aspergillus and Penicillium stimulation in cytokine release and gene expression profile in MD. Patients with MD showed higher basal levels of IL-1β, IL-1RA, IL-6 and TNF-α when compared to healthy controls. We observed that IL-1β levels had a bimodal distribution suggesting two different subgroups of patients, with low and high basal levels of cytokines. Gene expression profile in peripheral blood mononuclear cells (PBMC) showed significant differences in patients with high and low basal levels of IL-1β. We found that both mold extracts triggered a significant release of TNF-α in MD patients, which were not found in controls. Moreover, after mold stimulation, MD patients showed a different gene expression profile in PBMC, according to the basal levels of IL-1β. The results indicate that a subset of MD patients have higher basal levels of proinflammatory cytokines and the exposure to Aspergillus and Penicillium extracts may trigger additional TNF-α release and contribute to exacerbate inflammation.
format	article
author	Lidia Frejo Alvaro Gallego-Martinez Teresa Requena Eduardo Martin-Sanz Juan Carlos Amor-Dorado Andres Soto-Varela Sofia Santos-Perez Juan Manuel Espinosa-Sanchez Angel Batuecas-Caletrio Ismael Aran Jesus Fraile Marcos Rossi-Izquierdo Jose Antonio Lopez-Escamez
author_facet	Lidia Frejo Alvaro Gallego-Martinez Teresa Requena Eduardo Martin-Sanz Juan Carlos Amor-Dorado Andres Soto-Varela Sofia Santos-Perez Juan Manuel Espinosa-Sanchez Angel Batuecas-Caletrio Ismael Aran Jesus Fraile Marcos Rossi-Izquierdo Jose Antonio Lopez-Escamez
author_sort	Lidia Frejo
title	Proinflammatory cytokines and response to molds in mononuclear cells of patients with Meniere disease
title_short	Proinflammatory cytokines and response to molds in mononuclear cells of patients with Meniere disease
title_full	Proinflammatory cytokines and response to molds in mononuclear cells of patients with Meniere disease
title_fullStr	Proinflammatory cytokines and response to molds in mononuclear cells of patients with Meniere disease
title_full_unstemmed	Proinflammatory cytokines and response to molds in mononuclear cells of patients with Meniere disease
title_sort	proinflammatory cytokines and response to molds in mononuclear cells of patients with meniere disease
publisher	Nature Portfolio
publishDate	2018
url	https://doaj.org/article/8c2219bd572b4c5098c96c4491eaca20
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Proinflammatory cytokines and response to molds in mononuclear cells of patients with Meniere disease

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