Efficient generation of monoclonal antibodies against peptide in the context of MHCII using magnetic enrichment

Generating antibodies specific for the peptide–MHCII complexes has been challenging, with only a handful made to date. Here, the authors develop a more efficient approach to generate these antibodies, and demonstrate their potential in research and therapeutic applications.

Guardado en:

Detalles Bibliográficos
Autores principales:	Justin A. Spanier, Daniel R. Frederick, Justin J. Taylor, James R. Heffernan, Dmitri I. Kotov, Tijana Martinov, Kevin C. Osum, Jenna L. Ruggiero, Blake J. Rust, Samuel J. Landry, Marc K. Jenkins, James B. McLachlan, Brian T. Fife
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2016
Materias:	Science Q
Acceso en línea:	https://doaj.org/article/8c2e27eaab1b4cc89a8d7d85fe9db231
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:8c2e27eaab1b4cc89a8d7d85fe9db231
record_format	dspace
spelling	oai:doaj.org-article:8c2e27eaab1b4cc89a8d7d85fe9db2312021-12-02T16:50:55ZEfficient generation of monoclonal antibodies against peptide in the context of MHCII using magnetic enrichment10.1038/ncomms118042041-1723https://doaj.org/article/8c2e27eaab1b4cc89a8d7d85fe9db2312016-06-01T00:00:00Zhttps://doi.org/10.1038/ncomms11804https://doaj.org/toc/2041-1723Generating antibodies specific for the peptide–MHCII complexes has been challenging, with only a handful made to date. Here, the authors develop a more efficient approach to generate these antibodies, and demonstrate their potential in research and therapeutic applications.Justin A. SpanierDaniel R. FrederickJustin J. TaylorJames R. HeffernanDmitri I. KotovTijana MartinovKevin C. OsumJenna L. RuggieroBlake J. RustSamuel J. LandryMarc K. JenkinsJames B. McLachlanBrian T. FifeNature PortfolioarticleScienceQENNature Communications, Vol 7, Iss 1, Pp 1-11 (2016)
institution	DOAJ
collection	DOAJ
language	EN
topic	Science Q
spellingShingle	Science Q Justin A. Spanier Daniel R. Frederick Justin J. Taylor James R. Heffernan Dmitri I. Kotov Tijana Martinov Kevin C. Osum Jenna L. Ruggiero Blake J. Rust Samuel J. Landry Marc K. Jenkins James B. McLachlan Brian T. Fife Efficient generation of monoclonal antibodies against peptide in the context of MHCII using magnetic enrichment
description	Generating antibodies specific for the peptide–MHCII complexes has been challenging, with only a handful made to date. Here, the authors develop a more efficient approach to generate these antibodies, and demonstrate their potential in research and therapeutic applications.
format	article
author	Justin A. Spanier Daniel R. Frederick Justin J. Taylor James R. Heffernan Dmitri I. Kotov Tijana Martinov Kevin C. Osum Jenna L. Ruggiero Blake J. Rust Samuel J. Landry Marc K. Jenkins James B. McLachlan Brian T. Fife
author_facet	Justin A. Spanier Daniel R. Frederick Justin J. Taylor James R. Heffernan Dmitri I. Kotov Tijana Martinov Kevin C. Osum Jenna L. Ruggiero Blake J. Rust Samuel J. Landry Marc K. Jenkins James B. McLachlan Brian T. Fife
author_sort	Justin A. Spanier
title	Efficient generation of monoclonal antibodies against peptide in the context of MHCII using magnetic enrichment
title_short	Efficient generation of monoclonal antibodies against peptide in the context of MHCII using magnetic enrichment
title_full	Efficient generation of monoclonal antibodies against peptide in the context of MHCII using magnetic enrichment
title_fullStr	Efficient generation of monoclonal antibodies against peptide in the context of MHCII using magnetic enrichment
title_full_unstemmed	Efficient generation of monoclonal antibodies against peptide in the context of MHCII using magnetic enrichment
title_sort	efficient generation of monoclonal antibodies against peptide in the context of mhcii using magnetic enrichment
publisher	Nature Portfolio
publishDate	2016
url	https://doaj.org/article/8c2e27eaab1b4cc89a8d7d85fe9db231
work_keys_str_mv	AT justinaspanier efficientgenerationofmonoclonalantibodiesagainstpeptideinthecontextofmhciiusingmagneticenrichment AT danielrfrederick efficientgenerationofmonoclonalantibodiesagainstpeptideinthecontextofmhciiusingmagneticenrichment AT justinjtaylor efficientgenerationofmonoclonalantibodiesagainstpeptideinthecontextofmhciiusingmagneticenrichment AT jamesrheffernan efficientgenerationofmonoclonalantibodiesagainstpeptideinthecontextofmhciiusingmagneticenrichment AT dmitriikotov efficientgenerationofmonoclonalantibodiesagainstpeptideinthecontextofmhciiusingmagneticenrichment AT tijanamartinov efficientgenerationofmonoclonalantibodiesagainstpeptideinthecontextofmhciiusingmagneticenrichment AT kevincosum efficientgenerationofmonoclonalantibodiesagainstpeptideinthecontextofmhciiusingmagneticenrichment AT jennalruggiero efficientgenerationofmonoclonalantibodiesagainstpeptideinthecontextofmhciiusingmagneticenrichment AT blakejrust efficientgenerationofmonoclonalantibodiesagainstpeptideinthecontextofmhciiusingmagneticenrichment AT samueljlandry efficientgenerationofmonoclonalantibodiesagainstpeptideinthecontextofmhciiusingmagneticenrichment AT marckjenkins efficientgenerationofmonoclonalantibodiesagainstpeptideinthecontextofmhciiusingmagneticenrichment AT jamesbmclachlan efficientgenerationofmonoclonalantibodiesagainstpeptideinthecontextofmhciiusingmagneticenrichment AT briantfife efficientgenerationofmonoclonalantibodiesagainstpeptideinthecontextofmhciiusingmagneticenrichment
_version_	1718383051143118848

Efficient generation of monoclonal antibodies against peptide in the context of MHCII using magnetic enrichment

Ejemplares similares