Genome-wide DNA methylation analysis of the porcine hypothalamus-pituitary-ovary axis

Abstract Previous studies have suggested that DNA methylation in both CpG and CpH (where H = C, T or A) contexts plays a critical role in biological functions of different tissues. However, the genome-wide DNA methylation patterns of porcine hypothalamus-pituitary-ovary (HPO) tissues remain virtuall...

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Autores principales:	Xiao-Long Yuan, Zhe Zhang, Bin Li, Ning Gao, Hao Zhang, Per Torp Sangild, Jia-Qi Li
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2017
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/8c2e2a3da35f46b4b5a9c258caa3dccc
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spelling	oai:doaj.org-article:8c2e2a3da35f46b4b5a9c258caa3dccc2021-12-02T15:06:17ZGenome-wide DNA methylation analysis of the porcine hypothalamus-pituitary-ovary axis10.1038/s41598-017-04603-x2045-2322https://doaj.org/article/8c2e2a3da35f46b4b5a9c258caa3dccc2017-06-01T00:00:00Zhttps://doi.org/10.1038/s41598-017-04603-xhttps://doaj.org/toc/2045-2322Abstract Previous studies have suggested that DNA methylation in both CpG and CpH (where H = C, T or A) contexts plays a critical role in biological functions of different tissues. However, the genome-wide DNA methylation patterns of porcine hypothalamus-pituitary-ovary (HPO) tissues remain virtually unexplored. In this study, methylomes of HPO tissues were profiled to investigate their differences and similarities. We found that HPO methylomes displayed tissue-specific methylation patterns in both CpG and CpH contexts. At gene locations, the methylation and density of CpGs was negatively linked at transcription start sites but positively linked at transcription end sites. The densities of CpGs and CpHs at CpG island (CGI) locations were negatively correlated with their methylation. Moreover, the methylation interactions between CGIs and genes showed similar pattern in the CpG context but tissue-specific pattern in the CpH context. CpGs located in CGIs, upstream regions and exons were protected from methylation dynamics, whereas CGI shores, CGI shelves and intergenic regions were more likely to be targets of methylation changes. The methylation dynamics enriching in a tissue-specific manner appeared to maintain and establish the biological functions of HPO tissues. Our analyses provided valuable insights into the tissue-specific methylomes of porcine HPO tissues.Xiao-Long YuanZhe ZhangBin LiNing GaoHao ZhangPer Torp SangildJia-Qi LiNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 7, Iss 1, Pp 1-11 (2017)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Xiao-Long Yuan Zhe Zhang Bin Li Ning Gao Hao Zhang Per Torp Sangild Jia-Qi Li Genome-wide DNA methylation analysis of the porcine hypothalamus-pituitary-ovary axis
description	Abstract Previous studies have suggested that DNA methylation in both CpG and CpH (where H = C, T or A) contexts plays a critical role in biological functions of different tissues. However, the genome-wide DNA methylation patterns of porcine hypothalamus-pituitary-ovary (HPO) tissues remain virtually unexplored. In this study, methylomes of HPO tissues were profiled to investigate their differences and similarities. We found that HPO methylomes displayed tissue-specific methylation patterns in both CpG and CpH contexts. At gene locations, the methylation and density of CpGs was negatively linked at transcription start sites but positively linked at transcription end sites. The densities of CpGs and CpHs at CpG island (CGI) locations were negatively correlated with their methylation. Moreover, the methylation interactions between CGIs and genes showed similar pattern in the CpG context but tissue-specific pattern in the CpH context. CpGs located in CGIs, upstream regions and exons were protected from methylation dynamics, whereas CGI shores, CGI shelves and intergenic regions were more likely to be targets of methylation changes. The methylation dynamics enriching in a tissue-specific manner appeared to maintain and establish the biological functions of HPO tissues. Our analyses provided valuable insights into the tissue-specific methylomes of porcine HPO tissues.
format	article
author	Xiao-Long Yuan Zhe Zhang Bin Li Ning Gao Hao Zhang Per Torp Sangild Jia-Qi Li
author_facet	Xiao-Long Yuan Zhe Zhang Bin Li Ning Gao Hao Zhang Per Torp Sangild Jia-Qi Li
author_sort	Xiao-Long Yuan
title	Genome-wide DNA methylation analysis of the porcine hypothalamus-pituitary-ovary axis
title_short	Genome-wide DNA methylation analysis of the porcine hypothalamus-pituitary-ovary axis
title_full	Genome-wide DNA methylation analysis of the porcine hypothalamus-pituitary-ovary axis
title_fullStr	Genome-wide DNA methylation analysis of the porcine hypothalamus-pituitary-ovary axis
title_full_unstemmed	Genome-wide DNA methylation analysis of the porcine hypothalamus-pituitary-ovary axis
title_sort	genome-wide dna methylation analysis of the porcine hypothalamus-pituitary-ovary axis
publisher	Nature Portfolio
publishDate	2017
url	https://doaj.org/article/8c2e2a3da35f46b4b5a9c258caa3dccc
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Genome-wide DNA methylation analysis of the porcine hypothalamus-pituitary-ovary axis

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