Insights into gene expression changes under conditions that facilitate horizontal gene transfer (mating) of a model archaeon

Abstract Horizontal gene transfer is a means by which bacteria, archaea, and eukaryotes are able to trade DNA within and between species. While there are a variety of mechanisms through which this genetic exchange can take place, one means prevalent in the archaeon Haloferax volcanii involves the tr...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Andrea M. Makkay, Artemis S. Louyakis, Nikhil Ram-Mohan, Uri Gophna, J. Peter Gogarten, R. Thane Papke
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2020
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/8c2fd9ed0dbf4cc5a0e45f10c78966ae
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:8c2fd9ed0dbf4cc5a0e45f10c78966ae
record_format	dspace
spelling	oai:doaj.org-article:8c2fd9ed0dbf4cc5a0e45f10c78966ae2021-12-02T13:33:59ZInsights into gene expression changes under conditions that facilitate horizontal gene transfer (mating) of a model archaeon10.1038/s41598-020-79296-w2045-2322https://doaj.org/article/8c2fd9ed0dbf4cc5a0e45f10c78966ae2020-12-01T00:00:00Zhttps://doi.org/10.1038/s41598-020-79296-whttps://doaj.org/toc/2045-2322Abstract Horizontal gene transfer is a means by which bacteria, archaea, and eukaryotes are able to trade DNA within and between species. While there are a variety of mechanisms through which this genetic exchange can take place, one means prevalent in the archaeon Haloferax volcanii involves the transient formation of cytoplasmic bridges between cells and is referred to as mating. This process can result in the exchange of very large fragments of DNA between the participating cells. Genes governing the process of mating, including triggers to initiate mating, mechanisms of cell fusion, and DNA exchange, have yet to be characterized. We used a transcriptomic approach to gain a more detailed knowledge of how mating might transpire. By examining the differential expression of genes expressed in cells harvested from mating conditions on a filter over time and comparing them to those expressed in a shaking culture, we were able to identify genes and pathways potentially associated with mating. These analyses provide new insights into both the mechanisms and barriers of mating in Hfx. volcanii.Andrea M. MakkayArtemis S. LouyakisNikhil Ram-MohanUri GophnaJ. Peter GogartenR. Thane PapkeNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 10, Iss 1, Pp 1-16 (2020)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Andrea M. Makkay Artemis S. Louyakis Nikhil Ram-Mohan Uri Gophna J. Peter Gogarten R. Thane Papke Insights into gene expression changes under conditions that facilitate horizontal gene transfer (mating) of a model archaeon
description	Abstract Horizontal gene transfer is a means by which bacteria, archaea, and eukaryotes are able to trade DNA within and between species. While there are a variety of mechanisms through which this genetic exchange can take place, one means prevalent in the archaeon Haloferax volcanii involves the transient formation of cytoplasmic bridges between cells and is referred to as mating. This process can result in the exchange of very large fragments of DNA between the participating cells. Genes governing the process of mating, including triggers to initiate mating, mechanisms of cell fusion, and DNA exchange, have yet to be characterized. We used a transcriptomic approach to gain a more detailed knowledge of how mating might transpire. By examining the differential expression of genes expressed in cells harvested from mating conditions on a filter over time and comparing them to those expressed in a shaking culture, we were able to identify genes and pathways potentially associated with mating. These analyses provide new insights into both the mechanisms and barriers of mating in Hfx. volcanii.
format	article
author	Andrea M. Makkay Artemis S. Louyakis Nikhil Ram-Mohan Uri Gophna J. Peter Gogarten R. Thane Papke
author_facet	Andrea M. Makkay Artemis S. Louyakis Nikhil Ram-Mohan Uri Gophna J. Peter Gogarten R. Thane Papke
author_sort	Andrea M. Makkay
title	Insights into gene expression changes under conditions that facilitate horizontal gene transfer (mating) of a model archaeon
title_short	Insights into gene expression changes under conditions that facilitate horizontal gene transfer (mating) of a model archaeon
title_full	Insights into gene expression changes under conditions that facilitate horizontal gene transfer (mating) of a model archaeon
title_fullStr	Insights into gene expression changes under conditions that facilitate horizontal gene transfer (mating) of a model archaeon
title_full_unstemmed	Insights into gene expression changes under conditions that facilitate horizontal gene transfer (mating) of a model archaeon
title_sort	insights into gene expression changes under conditions that facilitate horizontal gene transfer (mating) of a model archaeon
publisher	Nature Portfolio
publishDate	2020
url	https://doaj.org/article/8c2fd9ed0dbf4cc5a0e45f10c78966ae
work_keys_str_mv	AT andreammakkay insightsintogeneexpressionchangesunderconditionsthatfacilitatehorizontalgenetransfermatingofamodelarchaeon AT artemisslouyakis insightsintogeneexpressionchangesunderconditionsthatfacilitatehorizontalgenetransfermatingofamodelarchaeon AT nikhilrammohan insightsintogeneexpressionchangesunderconditionsthatfacilitatehorizontalgenetransfermatingofamodelarchaeon AT urigophna insightsintogeneexpressionchangesunderconditionsthatfacilitatehorizontalgenetransfermatingofamodelarchaeon AT jpetergogarten insightsintogeneexpressionchangesunderconditionsthatfacilitatehorizontalgenetransfermatingofamodelarchaeon AT rthanepapke insightsintogeneexpressionchangesunderconditionsthatfacilitatehorizontalgenetransfermatingofamodelarchaeon
_version_	1718392807268286464

Insights into gene expression changes under conditions that facilitate horizontal gene transfer (mating) of a model archaeon

Ejemplares similares