Protocol for fungal infection following the induction of epithelial cell loss in larval zebrafish

Summary: Epithelia provide the first line of defense against foreign pathogens, and disruption of tissue homeostasis frequently allows for opportunistic infections. Here we provide a protocol for induction of epithelial cell loss in zebrafish larvae, followed by infection with fungal pathogens. Deta...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Sebastian Wurster, Oscar E. Ruiz, Alexander M. Tatara, Dimitrios P. Kontoyiannis, George T. Eisenhoffer
Formato:	article
Lenguaje:	EN
Publicado:	Elsevier 2021
Materias:	Cell Biology Microbiology Model Organisms Molecular Biology Science (General) Q1-390
Acceso en línea:	https://doaj.org/article/8c3176e5094d431eac1fff5bd8545e84
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:8c3176e5094d431eac1fff5bd8545e84
record_format	dspace
spelling	oai:doaj.org-article:8c3176e5094d431eac1fff5bd8545e842021-11-22T04:30:57ZProtocol for fungal infection following the induction of epithelial cell loss in larval zebrafish2666-166710.1016/j.xpro.2021.100963https://doaj.org/article/8c3176e5094d431eac1fff5bd8545e842021-12-01T00:00:00Zhttp://www.sciencedirect.com/science/article/pii/S2666166721006699https://doaj.org/toc/2666-1667Summary: Epithelia provide the first line of defense against foreign pathogens, and disruption of tissue homeostasis frequently allows for opportunistic infections. Here we provide a protocol for induction of epithelial cell loss in zebrafish larvae, followed by infection with fungal pathogens. Details are provided for monitoring larval survival after infection, assessment of fungal burden, and prophylactic treatment with antifungal compounds. Limitations of the protocol include potential antifungal toxicity and high fungal inoculums to induce lethal infection with some pathogenic fungal species.For complete details on the use and execution of this protocol, please refer to Wurster et al. (2021).Sebastian WursterOscar E. RuizAlexander M. TataraDimitrios P. KontoyiannisGeorge T. EisenhofferElsevierarticleCell BiologyMicrobiologyModel OrganismsMolecular BiologyScience (General)Q1-390ENSTAR Protocols, Vol 2, Iss 4, Pp 100963- (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Cell Biology Microbiology Model Organisms Molecular Biology Science (General) Q1-390
spellingShingle	Cell Biology Microbiology Model Organisms Molecular Biology Science (General) Q1-390 Sebastian Wurster Oscar E. Ruiz Alexander M. Tatara Dimitrios P. Kontoyiannis George T. Eisenhoffer Protocol for fungal infection following the induction of epithelial cell loss in larval zebrafish
description	Summary: Epithelia provide the first line of defense against foreign pathogens, and disruption of tissue homeostasis frequently allows for opportunistic infections. Here we provide a protocol for induction of epithelial cell loss in zebrafish larvae, followed by infection with fungal pathogens. Details are provided for monitoring larval survival after infection, assessment of fungal burden, and prophylactic treatment with antifungal compounds. Limitations of the protocol include potential antifungal toxicity and high fungal inoculums to induce lethal infection with some pathogenic fungal species.For complete details on the use and execution of this protocol, please refer to Wurster et al. (2021).
format	article
author	Sebastian Wurster Oscar E. Ruiz Alexander M. Tatara Dimitrios P. Kontoyiannis George T. Eisenhoffer
author_facet	Sebastian Wurster Oscar E. Ruiz Alexander M. Tatara Dimitrios P. Kontoyiannis George T. Eisenhoffer
author_sort	Sebastian Wurster
title	Protocol for fungal infection following the induction of epithelial cell loss in larval zebrafish
title_short	Protocol for fungal infection following the induction of epithelial cell loss in larval zebrafish
title_full	Protocol for fungal infection following the induction of epithelial cell loss in larval zebrafish
title_fullStr	Protocol for fungal infection following the induction of epithelial cell loss in larval zebrafish
title_full_unstemmed	Protocol for fungal infection following the induction of epithelial cell loss in larval zebrafish
title_sort	protocol for fungal infection following the induction of epithelial cell loss in larval zebrafish
publisher	Elsevier
publishDate	2021
url	https://doaj.org/article/8c3176e5094d431eac1fff5bd8545e84
work_keys_str_mv	AT sebastianwurster protocolforfungalinfectionfollowingtheinductionofepithelialcelllossinlarvalzebrafish AT oscareruiz protocolforfungalinfectionfollowingtheinductionofepithelialcelllossinlarvalzebrafish AT alexandermtatara protocolforfungalinfectionfollowingtheinductionofepithelialcelllossinlarvalzebrafish AT dimitriospkontoyiannis protocolforfungalinfectionfollowingtheinductionofepithelialcelllossinlarvalzebrafish AT georgeteisenhoffer protocolforfungalinfectionfollowingtheinductionofepithelialcelllossinlarvalzebrafish
_version_	1718418174472355840

Protocol for fungal infection following the induction of epithelial cell loss in larval zebrafish

Ejemplares similares