CryoEM reveals the stochastic nature of individual ATP binding events in a group II chaperonin

The mechanism by which chaperonins coordinate ATP utilization in their multiple subunits remains unclear. Here, the authors employ an approach that uses cryo-EM single particle analysis to track the number and distribution of nucleotides bound to each subunit in the homo-oligomeric MmCpn archaeal ch...

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Autores principales:	Yanyan Zhao, Michael F. Schmid, Judith Frydman, Wah Chiu
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Science Q
Acceso en línea:	https://doaj.org/article/8c38f29ae54c40088fd5bbdd48cbf038
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spelling	oai:doaj.org-article:8c38f29ae54c40088fd5bbdd48cbf0382021-12-02T18:49:37ZCryoEM reveals the stochastic nature of individual ATP binding events in a group II chaperonin10.1038/s41467-021-25099-02041-1723https://doaj.org/article/8c38f29ae54c40088fd5bbdd48cbf0382021-08-01T00:00:00Zhttps://doi.org/10.1038/s41467-021-25099-0https://doaj.org/toc/2041-1723The mechanism by which chaperonins coordinate ATP utilization in their multiple subunits remains unclear. Here, the authors employ an approach that uses cryo-EM single particle analysis to track the number and distribution of nucleotides bound to each subunit in the homo-oligomeric MmCpn archaeal chaperonin complex and observe that ATP binds in a statistically random manner to MmCpn both within a ring and across the rings, which shows that there is no cooperativity in ATP binding to archaeal group II chaperonins under the conditions used in this study.Yanyan ZhaoMichael F. SchmidJudith FrydmanWah ChiuNature PortfolioarticleScienceQENNature Communications, Vol 12, Iss 1, Pp 1-10 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Science Q
spellingShingle	Science Q Yanyan Zhao Michael F. Schmid Judith Frydman Wah Chiu CryoEM reveals the stochastic nature of individual ATP binding events in a group II chaperonin
description	The mechanism by which chaperonins coordinate ATP utilization in their multiple subunits remains unclear. Here, the authors employ an approach that uses cryo-EM single particle analysis to track the number and distribution of nucleotides bound to each subunit in the homo-oligomeric MmCpn archaeal chaperonin complex and observe that ATP binds in a statistically random manner to MmCpn both within a ring and across the rings, which shows that there is no cooperativity in ATP binding to archaeal group II chaperonins under the conditions used in this study.
format	article
author	Yanyan Zhao Michael F. Schmid Judith Frydman Wah Chiu
author_facet	Yanyan Zhao Michael F. Schmid Judith Frydman Wah Chiu
author_sort	Yanyan Zhao
title	CryoEM reveals the stochastic nature of individual ATP binding events in a group II chaperonin
title_short	CryoEM reveals the stochastic nature of individual ATP binding events in a group II chaperonin
title_full	CryoEM reveals the stochastic nature of individual ATP binding events in a group II chaperonin
title_fullStr	CryoEM reveals the stochastic nature of individual ATP binding events in a group II chaperonin
title_full_unstemmed	CryoEM reveals the stochastic nature of individual ATP binding events in a group II chaperonin
title_sort	cryoem reveals the stochastic nature of individual atp binding events in a group ii chaperonin
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/8c38f29ae54c40088fd5bbdd48cbf038
work_keys_str_mv	AT yanyanzhao cryoemrevealsthestochasticnatureofindividualatpbindingeventsinagroupiichaperonin AT michaelfschmid cryoemrevealsthestochasticnatureofindividualatpbindingeventsinagroupiichaperonin AT judithfrydman cryoemrevealsthestochasticnatureofindividualatpbindingeventsinagroupiichaperonin AT wahchiu cryoemrevealsthestochasticnatureofindividualatpbindingeventsinagroupiichaperonin
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CryoEM reveals the stochastic nature of individual ATP binding events in a group II chaperonin

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