Herb-Partitioned Moxibustion Improves Crohn’s Disease-Associated Intestinal Fibrosis by Suppressing the RhoA/ROCK1/MLC Pathway

Background and Aims. Intestinal fibrosis is one of the severe and common complications of Crohn’s disease (CD), but the etiology and pathogenesis remain uncertain. The study intended to examine whether the effect of herb-partitioned moxibustion on rats with CD-associated intestinal fibrosis is assoc...

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Autores principales:	Min Zhao, Zhijun Weng, Yan Huang, Handan Zheng, Dong Han, Jiacheng Shen, Rong Huang, Huirong Liu, Luyi Wu
Formato:	article
Lenguaje:	EN
Publicado:	Hindawi Limited 2021
Materias:	Other systems of medicine RZ201-999
Acceso en línea:	https://doaj.org/article/8c3ee1ed26c243cea77046d914a194ab
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spelling	oai:doaj.org-article:8c3ee1ed26c243cea77046d914a194ab2021-11-29T00:55:36ZHerb-Partitioned Moxibustion Improves Crohn’s Disease-Associated Intestinal Fibrosis by Suppressing the RhoA/ROCK1/MLC Pathway1741-428810.1155/2021/2247953https://doaj.org/article/8c3ee1ed26c243cea77046d914a194ab2021-01-01T00:00:00Zhttp://dx.doi.org/10.1155/2021/2247953https://doaj.org/toc/1741-4288Background and Aims. Intestinal fibrosis is one of the severe and common complications of Crohn’s disease (CD), but the etiology and pathogenesis remain uncertain. The study intended to examine whether the effect of herb-partitioned moxibustion on rats with CD-associated intestinal fibrosis is associated with the RhoA/ROCK1/MLC pathway. Methods. All experimental rats were randomly allocated into the normal control group (NC), model control group (MC), and herb-partitioned moxibustion group (HPM). Intestinal fibrosis was established in rats with CD by repeated rectal administrations of 2,4,6-trinitrobenzenesulfonic acid (TNBS). Herb-partitioned moxibustion was applied at the Qihai (CV6) and Tianshu (ST25) acupoints once daily for 10 days in the HPM group. In this study, histological changes were examined by hematoxylin and eosin (HE) staining; then, Masson’s trichrome staining was used to assess the degree of fibrosis in each group. Experimental methods of immunohistochemistry, western blotting, and real-time PCR were applied to detect the levels of α-SMA, collagen III, RhoA, ROCK1, and p-MLC. Moreover, the double immunofluorescent staining for the colocalization of both α-SMA and ROCK1 was performed. Results. Contrasted with the normal controls, the collagen deposition and fibrosis scores were increased in colonic tissue of model rats, and HPM decreased the collagen deposition and fibrosis scores. The protein of α-SMA and collagen III in the MC group exceeds that of the NC group; HPM decreased the expression of α-SMA and collagen III in rats with intestinal fibrosis. Similarly, the expression of RhoA, ROCK1, and p-MLC in model rats was obviously increased compared with normal controls; the expression of RhoA, ROCK1, and p-MLC was decreased after HPM. The coexpression of α-SMA and ROCK1 in rats with intestinal fibrosis was higher than normal rats. Conclusion. HPM improves CD-associated intestinal fibrosis by suppressing the RhoA/ROCK1/MLC pathway.Min ZhaoZhijun WengYan HuangHandan ZhengDong HanJiacheng ShenRong HuangHuirong LiuLuyi WuHindawi LimitedarticleOther systems of medicineRZ201-999ENEvidence-Based Complementary and Alternative Medicine, Vol 2021 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Other systems of medicine RZ201-999
spellingShingle	Other systems of medicine RZ201-999 Min Zhao Zhijun Weng Yan Huang Handan Zheng Dong Han Jiacheng Shen Rong Huang Huirong Liu Luyi Wu Herb-Partitioned Moxibustion Improves Crohn’s Disease-Associated Intestinal Fibrosis by Suppressing the RhoA/ROCK1/MLC Pathway
description	Background and Aims. Intestinal fibrosis is one of the severe and common complications of Crohn’s disease (CD), but the etiology and pathogenesis remain uncertain. The study intended to examine whether the effect of herb-partitioned moxibustion on rats with CD-associated intestinal fibrosis is associated with the RhoA/ROCK1/MLC pathway. Methods. All experimental rats were randomly allocated into the normal control group (NC), model control group (MC), and herb-partitioned moxibustion group (HPM). Intestinal fibrosis was established in rats with CD by repeated rectal administrations of 2,4,6-trinitrobenzenesulfonic acid (TNBS). Herb-partitioned moxibustion was applied at the Qihai (CV6) and Tianshu (ST25) acupoints once daily for 10 days in the HPM group. In this study, histological changes were examined by hematoxylin and eosin (HE) staining; then, Masson’s trichrome staining was used to assess the degree of fibrosis in each group. Experimental methods of immunohistochemistry, western blotting, and real-time PCR were applied to detect the levels of α-SMA, collagen III, RhoA, ROCK1, and p-MLC. Moreover, the double immunofluorescent staining for the colocalization of both α-SMA and ROCK1 was performed. Results. Contrasted with the normal controls, the collagen deposition and fibrosis scores were increased in colonic tissue of model rats, and HPM decreased the collagen deposition and fibrosis scores. The protein of α-SMA and collagen III in the MC group exceeds that of the NC group; HPM decreased the expression of α-SMA and collagen III in rats with intestinal fibrosis. Similarly, the expression of RhoA, ROCK1, and p-MLC in model rats was obviously increased compared with normal controls; the expression of RhoA, ROCK1, and p-MLC was decreased after HPM. The coexpression of α-SMA and ROCK1 in rats with intestinal fibrosis was higher than normal rats. Conclusion. HPM improves CD-associated intestinal fibrosis by suppressing the RhoA/ROCK1/MLC pathway.
format	article
author	Min Zhao Zhijun Weng Yan Huang Handan Zheng Dong Han Jiacheng Shen Rong Huang Huirong Liu Luyi Wu
author_facet	Min Zhao Zhijun Weng Yan Huang Handan Zheng Dong Han Jiacheng Shen Rong Huang Huirong Liu Luyi Wu
author_sort	Min Zhao
title	Herb-Partitioned Moxibustion Improves Crohn’s Disease-Associated Intestinal Fibrosis by Suppressing the RhoA/ROCK1/MLC Pathway
title_short	Herb-Partitioned Moxibustion Improves Crohn’s Disease-Associated Intestinal Fibrosis by Suppressing the RhoA/ROCK1/MLC Pathway
title_full	Herb-Partitioned Moxibustion Improves Crohn’s Disease-Associated Intestinal Fibrosis by Suppressing the RhoA/ROCK1/MLC Pathway
title_fullStr	Herb-Partitioned Moxibustion Improves Crohn’s Disease-Associated Intestinal Fibrosis by Suppressing the RhoA/ROCK1/MLC Pathway
title_full_unstemmed	Herb-Partitioned Moxibustion Improves Crohn’s Disease-Associated Intestinal Fibrosis by Suppressing the RhoA/ROCK1/MLC Pathway
title_sort	herb-partitioned moxibustion improves crohn’s disease-associated intestinal fibrosis by suppressing the rhoa/rock1/mlc pathway
publisher	Hindawi Limited
publishDate	2021
url	https://doaj.org/article/8c3ee1ed26c243cea77046d914a194ab
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Herb-Partitioned Moxibustion Improves Crohn’s Disease-Associated Intestinal Fibrosis by Suppressing the RhoA/ROCK1/MLC Pathway

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