Crossover localisation is regulated by the neddylation posttranslational regulatory pathway.

Crossovers (COs) are at the origin of genetic variability, occurring across successive generations, and they are also essential for the correct segregation of chromosomes during meiosis. Their number and position are precisely controlled, however the mechanisms underlying these controls are poorly u...

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Autores principales:	Marina Tagliaro Jahns, Daniel Vezon, Aurélie Chambon, Lucie Pereira, Matthieu Falque, Olivier C Martin, Liudmila Chelysheva, Mathilde Grelon
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2014
Materias:	Biology (General) QH301-705.5
Acceso en línea:	https://doaj.org/article/8c4dddc23be84bf783334e71e547f2cf
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spelling	oai:doaj.org-article:8c4dddc23be84bf783334e71e547f2cf2021-11-25T05:33:00ZCrossover localisation is regulated by the neddylation posttranslational regulatory pathway.1544-91731545-788510.1371/journal.pbio.1001930https://doaj.org/article/8c4dddc23be84bf783334e71e547f2cf2014-08-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/25116939/?tool=EBIhttps://doaj.org/toc/1544-9173https://doaj.org/toc/1545-7885Crossovers (COs) are at the origin of genetic variability, occurring across successive generations, and they are also essential for the correct segregation of chromosomes during meiosis. Their number and position are precisely controlled, however the mechanisms underlying these controls are poorly understood. Neddylation/rubylation is a regulatory pathway of posttranslational protein modification that is required for numerous cellular processes in eukaryotes, but has not yet been linked to homologous recombination. In a screen for meiotic recombination-defective mutants, we identified several axr1 alleles, disrupting the gene encoding the E1 enzyme of the neddylation complex in Arabidopsis. Using genetic and cytological approaches we found that axr1 mutants are characterised by a shortage in bivalent formation correlated with strong synapsis defects. We determined that the bivalent shortage in axr1 is not due to a general decrease in CO formation but rather due to a mislocalisation of class I COs. In axr1, as in wild type, COs are still under the control of the ZMM group of proteins. However, in contrast to wild type, they tend to cluster together and no longer follow the obligatory CO rule. Lastly, we showed that this deregulation of CO localisation is likely to be mediated by the activity of a cullin 4 RING ligase, known to be involved in DNA damage sensing during somatic DNA repair and mouse spermatogenesis. In conclusion, we provide evidence that the neddylation/rubylation pathway of protein modification is a key regulator of meiotic recombination. We propose that rather than regulating the number of recombination events, this pathway regulates their localisation, through the activation of cullin 4 RING ligase complexes. Possible targets for these ligases are discussed.Marina Tagliaro JahnsDaniel VezonAurélie ChambonLucie PereiraMatthieu FalqueOlivier C MartinLiudmila ChelyshevaMathilde GrelonPublic Library of Science (PLoS)articleBiology (General)QH301-705.5ENPLoS Biology, Vol 12, Iss 8, p e1001930 (2014)
institution	DOAJ
collection	DOAJ
language	EN
topic	Biology (General) QH301-705.5
spellingShingle	Biology (General) QH301-705.5 Marina Tagliaro Jahns Daniel Vezon Aurélie Chambon Lucie Pereira Matthieu Falque Olivier C Martin Liudmila Chelysheva Mathilde Grelon Crossover localisation is regulated by the neddylation posttranslational regulatory pathway.
description	Crossovers (COs) are at the origin of genetic variability, occurring across successive generations, and they are also essential for the correct segregation of chromosomes during meiosis. Their number and position are precisely controlled, however the mechanisms underlying these controls are poorly understood. Neddylation/rubylation is a regulatory pathway of posttranslational protein modification that is required for numerous cellular processes in eukaryotes, but has not yet been linked to homologous recombination. In a screen for meiotic recombination-defective mutants, we identified several axr1 alleles, disrupting the gene encoding the E1 enzyme of the neddylation complex in Arabidopsis. Using genetic and cytological approaches we found that axr1 mutants are characterised by a shortage in bivalent formation correlated with strong synapsis defects. We determined that the bivalent shortage in axr1 is not due to a general decrease in CO formation but rather due to a mislocalisation of class I COs. In axr1, as in wild type, COs are still under the control of the ZMM group of proteins. However, in contrast to wild type, they tend to cluster together and no longer follow the obligatory CO rule. Lastly, we showed that this deregulation of CO localisation is likely to be mediated by the activity of a cullin 4 RING ligase, known to be involved in DNA damage sensing during somatic DNA repair and mouse spermatogenesis. In conclusion, we provide evidence that the neddylation/rubylation pathway of protein modification is a key regulator of meiotic recombination. We propose that rather than regulating the number of recombination events, this pathway regulates their localisation, through the activation of cullin 4 RING ligase complexes. Possible targets for these ligases are discussed.
format	article
author	Marina Tagliaro Jahns Daniel Vezon Aurélie Chambon Lucie Pereira Matthieu Falque Olivier C Martin Liudmila Chelysheva Mathilde Grelon
author_facet	Marina Tagliaro Jahns Daniel Vezon Aurélie Chambon Lucie Pereira Matthieu Falque Olivier C Martin Liudmila Chelysheva Mathilde Grelon
author_sort	Marina Tagliaro Jahns
title	Crossover localisation is regulated by the neddylation posttranslational regulatory pathway.
title_short	Crossover localisation is regulated by the neddylation posttranslational regulatory pathway.
title_full	Crossover localisation is regulated by the neddylation posttranslational regulatory pathway.
title_fullStr	Crossover localisation is regulated by the neddylation posttranslational regulatory pathway.
title_full_unstemmed	Crossover localisation is regulated by the neddylation posttranslational regulatory pathway.
title_sort	crossover localisation is regulated by the neddylation posttranslational regulatory pathway.
publisher	Public Library of Science (PLoS)
publishDate	2014
url	https://doaj.org/article/8c4dddc23be84bf783334e71e547f2cf
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Crossover localisation is regulated by the neddylation posttranslational regulatory pathway.

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