Molecular Exploration of Guava (Psidium guajava L.) Genome Using SSR and RAPD Markers: A Step towards Establishing Linkage Map

In the present study, molecular evaluation of two guava mapping populations (MP), MPI comprising 94 F1 progenies and MPII comprising 46 F1 progenies, was carried out using simple sequence repeat (SSR) and random amplified polymorphic DNA (RAPD) markers. A pseudo-test cross strategy was implemented w...

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Autores principales:	B Padmakar, D Sailaja, C Aswath
Formato:	article
Lenguaje:	EN
Publicado:	Society for Promotion of Horticulture - Indian Institute of Horticultural Research 2015
Materias:	guava genotyping m13-tailed pcr ssr rapd Plant culture SB1-1110
Acceso en línea:	https://doaj.org/article/8c52f99b6a5a4220ba2dbf9a0b5bcf4f
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spelling	oai:doaj.org-article:8c52f99b6a5a4220ba2dbf9a0b5bcf4f2021-12-02T10:35:44ZMolecular Exploration of Guava (Psidium guajava L.) Genome Using SSR and RAPD Markers: A Step towards Establishing Linkage Map0973-354X2582-4899https://doaj.org/article/8c52f99b6a5a4220ba2dbf9a0b5bcf4f2015-12-01T00:00:00Zhttps://jhs.iihr.res.in/index.php/jhs/article/view/118https://doaj.org/toc/0973-354Xhttps://doaj.org/toc/2582-4899In the present study, molecular evaluation of two guava mapping populations (MP), MPI comprising 94 F1 progenies and MPII comprising 46 F1 progenies, was carried out using simple sequence repeat (SSR) and random amplified polymorphic DNA (RAPD) markers. A pseudo-test cross strategy was implemented where 'Kamsari' X 'Purple Local' and 'Purple Local' X 'Allahabad Safeda' were crossed, and, these showed variation in fruit quality traits such as seedstrength (hardness/softness), fruit weight, TSS and pulp color. A set of 30 RAPD markers was used for genotyping MPI while a set of 55 SSR markers was used for genotyping MPII. In case of MPI, 30 RAPD markers generated 214 scorable markers, of which 80 markers were specific to 'Kamsari', 14 markers to 'Purple Local' and the remaining 120 were intercross markers. As for MPII, 55 polymorphic SSR markers resulted in generation of 207 alleles (with a maximum of 4 alleles and a minimum of 3 alleles per locus), of which 108 alleles were specific to 'Purple Local' while 99 were specific to 'Allahabad Safeda'. Genotypic data thus generated can be further exploited for constructing genetic linkage maps and mapping complex QTLs governing fruit quality traits in guava..B PadmakarD SailajaC AswathSociety for Promotion of Horticulture - Indian Institute of Horticultural Researcharticleguavagenotypingm13-tailed pcrssrrapdPlant cultureSB1-1110ENJournal of Horticultural Sciences, Vol 10, Iss 2, Pp 130-135 (2015)
institution	DOAJ
collection	DOAJ
language	EN
topic	guava genotyping m13-tailed pcr ssr rapd Plant culture SB1-1110
spellingShingle	guava genotyping m13-tailed pcr ssr rapd Plant culture SB1-1110 B Padmakar D Sailaja C Aswath Molecular Exploration of Guava (Psidium guajava L.) Genome Using SSR and RAPD Markers: A Step towards Establishing Linkage Map
description	In the present study, molecular evaluation of two guava mapping populations (MP), MPI comprising 94 F1 progenies and MPII comprising 46 F1 progenies, was carried out using simple sequence repeat (SSR) and random amplified polymorphic DNA (RAPD) markers. A pseudo-test cross strategy was implemented where 'Kamsari' X 'Purple Local' and 'Purple Local' X 'Allahabad Safeda' were crossed, and, these showed variation in fruit quality traits such as seedstrength (hardness/softness), fruit weight, TSS and pulp color. A set of 30 RAPD markers was used for genotyping MPI while a set of 55 SSR markers was used for genotyping MPII. In case of MPI, 30 RAPD markers generated 214 scorable markers, of which 80 markers were specific to 'Kamsari', 14 markers to 'Purple Local' and the remaining 120 were intercross markers. As for MPII, 55 polymorphic SSR markers resulted in generation of 207 alleles (with a maximum of 4 alleles and a minimum of 3 alleles per locus), of which 108 alleles were specific to 'Purple Local' while 99 were specific to 'Allahabad Safeda'. Genotypic data thus generated can be further exploited for constructing genetic linkage maps and mapping complex QTLs governing fruit quality traits in guava..
format	article
author	B Padmakar D Sailaja C Aswath
author_facet	B Padmakar D Sailaja C Aswath
author_sort	B Padmakar
title	Molecular Exploration of Guava (Psidium guajava L.) Genome Using SSR and RAPD Markers: A Step towards Establishing Linkage Map
title_short	Molecular Exploration of Guava (Psidium guajava L.) Genome Using SSR and RAPD Markers: A Step towards Establishing Linkage Map
title_full	Molecular Exploration of Guava (Psidium guajava L.) Genome Using SSR and RAPD Markers: A Step towards Establishing Linkage Map
title_fullStr	Molecular Exploration of Guava (Psidium guajava L.) Genome Using SSR and RAPD Markers: A Step towards Establishing Linkage Map
title_full_unstemmed	Molecular Exploration of Guava (Psidium guajava L.) Genome Using SSR and RAPD Markers: A Step towards Establishing Linkage Map
title_sort	molecular exploration of guava (psidium guajava l.) genome using ssr and rapd markers: a step towards establishing linkage map
publisher	Society for Promotion of Horticulture - Indian Institute of Horticultural Research
publishDate	2015
url	https://doaj.org/article/8c52f99b6a5a4220ba2dbf9a0b5bcf4f
work_keys_str_mv	AT bpadmakar molecularexplorationofguavapsidiumguajavalgenomeusingssrandrapdmarkersasteptowardsestablishinglinkagemap AT dsailaja molecularexplorationofguavapsidiumguajavalgenomeusingssrandrapdmarkersasteptowardsestablishinglinkagemap AT caswath molecularexplorationofguavapsidiumguajavalgenomeusingssrandrapdmarkersasteptowardsestablishinglinkagemap
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Molecular Exploration of Guava (Psidium guajava L.) Genome Using SSR and RAPD Markers: A Step towards Establishing Linkage Map

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