Acid selective pro-dye for cellular compartments

Abstract A novel pro-dye approach for the acid-selective staining of the subcellular compartments for better permeability and selectivity was applied. The designed sensor has suitable physicochemical properties such as a large Stokes shift and a long-lived intracellular fluorescence. The Schiff base...

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Autores principales:	Barbara Czaplińska, Katarzyna Malarz, Anna Mrozek-Wilczkiewicz, Robert Musiol
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2019
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/8c5916d708804c0bbfd70347e85d60a5
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spelling	oai:doaj.org-article:8c5916d708804c0bbfd70347e85d60a52021-12-02T15:09:54ZAcid selective pro-dye for cellular compartments10.1038/s41598-019-50734-82045-2322https://doaj.org/article/8c5916d708804c0bbfd70347e85d60a52019-10-01T00:00:00Zhttps://doi.org/10.1038/s41598-019-50734-8https://doaj.org/toc/2045-2322Abstract A novel pro-dye approach for the acid-selective staining of the subcellular compartments for better permeability and selectivity was applied. The designed sensor has suitable physicochemical properties such as a large Stokes shift and a long-lived intracellular fluorescence. The Schiff base fragment was used for the acid-sensitive release of a fluorophore without affecting the overall stability of the biological systems. Due to the presence of an imine bond in its structure and its unique fluorescent properties, it can be presented as a “pro-dye” for acidic structures such as lysosomes. As a result of an imine bond cleavage, a new fluorescent compound is released, whose substantially shifted excitation and emission wavelengths enable a more selective and effective imaging of lysosomes and endosomes. The presented report provides the chemical, physicochemical and optical profiles as well as biological assays and theoretical calculations.Barbara CzaplińskaKatarzyna MalarzAnna Mrozek-WilczkiewiczRobert MusiolNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 9, Iss 1, Pp 1-9 (2019)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Barbara Czaplińska Katarzyna Malarz Anna Mrozek-Wilczkiewicz Robert Musiol Acid selective pro-dye for cellular compartments
description	Abstract A novel pro-dye approach for the acid-selective staining of the subcellular compartments for better permeability and selectivity was applied. The designed sensor has suitable physicochemical properties such as a large Stokes shift and a long-lived intracellular fluorescence. The Schiff base fragment was used for the acid-sensitive release of a fluorophore without affecting the overall stability of the biological systems. Due to the presence of an imine bond in its structure and its unique fluorescent properties, it can be presented as a “pro-dye” for acidic structures such as lysosomes. As a result of an imine bond cleavage, a new fluorescent compound is released, whose substantially shifted excitation and emission wavelengths enable a more selective and effective imaging of lysosomes and endosomes. The presented report provides the chemical, physicochemical and optical profiles as well as biological assays and theoretical calculations.
format	article
author	Barbara Czaplińska Katarzyna Malarz Anna Mrozek-Wilczkiewicz Robert Musiol
author_facet	Barbara Czaplińska Katarzyna Malarz Anna Mrozek-Wilczkiewicz Robert Musiol
author_sort	Barbara Czaplińska
title	Acid selective pro-dye for cellular compartments
title_short	Acid selective pro-dye for cellular compartments
title_full	Acid selective pro-dye for cellular compartments
title_fullStr	Acid selective pro-dye for cellular compartments
title_full_unstemmed	Acid selective pro-dye for cellular compartments
title_sort	acid selective pro-dye for cellular compartments
publisher	Nature Portfolio
publishDate	2019
url	https://doaj.org/article/8c5916d708804c0bbfd70347e85d60a5
work_keys_str_mv	AT barbaraczaplinska acidselectiveprodyeforcellularcompartments AT katarzynamalarz acidselectiveprodyeforcellularcompartments AT annamrozekwilczkiewicz acidselectiveprodyeforcellularcompartments AT robertmusiol acidselectiveprodyeforcellularcompartments
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Acid selective pro-dye for cellular compartments

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