Detection of Biocides Resistance Genes among Clinical Isolates of Both Methicillin-Resistant and Methicillin-Sensitive Strains of Staphylococcus Aureus

BACKGROUND: Biocides compounds are widely used in infection control programs, including hand washing and skin decolonization before invasive procedures. However, its overuse leads to the emergence of Staphylococcus aureus with decreased biocides susceptibility, which became a problem in hospitals in...

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Autores principales:	Walid Sabry, Mohammed Yosry Al-Dakhakhni, Ibrahim Al-hoseiny, Raafat Abd Rabbo Abdel Tawab
Formato:	article
Lenguaje:	EN
Publicado:	Al-Azhar University, Faculty of Medicine (Damietta) 2021
Materias:	resistance biocides staphylococcus aureus methicillin-resistant biocides’ resistance genes Medicine (General) R5-920
Acceso en línea:	https://doaj.org/article/8c5a99e3fcba40fba6bf53b02138bcad
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spelling	oai:doaj.org-article:8c5a99e3fcba40fba6bf53b02138bcad2021-12-02T19:02:51ZDetection of Biocides Resistance Genes among Clinical Isolates of Both Methicillin-Resistant and Methicillin-Sensitive Strains of Staphylococcus Aureus2636-41742682-378010.21608/ijma.2021.62186.1260https://doaj.org/article/8c5a99e3fcba40fba6bf53b02138bcad2021-07-01T00:00:00Zhttps://ijma.journals.ekb.eg/article_177074_c755a36efed7e5845fec1d35e4d40d78.pdfhttps://doaj.org/toc/2636-4174https://doaj.org/toc/2682-3780BACKGROUND: Biocides compounds are widely used in infection control programs, including hand washing and skin decolonization before invasive procedures. However, its overuse leads to the emergence of Staphylococcus aureus with decreased biocides susceptibility, which became a problem in hospitals in different parts of the world. AIM OF THE WORK: The present work aims to study the susceptibility of methicillin-resistant isolates of staphylococci aureus, obtained from different clinical samples to commonly used biocides, determines the prevalence of the biocide resistance genes, qacA/B, and qacC, among these isolates. METHODS: Biocide’s resistance genes [qacA/B, qacC] in Both Methicillin-Resistant and Methicillin-Sensitive Strains of Staphylococcus aureus were detected by PCR method. The minimum inhibitory concentrations [MICs] of Biocide agents were determined by broth dilution method, whereas antibiotic susceptibility was analyzed by disk diffusion and MICs methods according to Clinical and Laboratory Standards Institute [CLSI] criteria. RESULTS: The frequency of Biocides resistance genes [qacA/B, qacC] was found to be high in our clinical staphylococci isolates. The qacA/B gene was detected in [63.3%] of MRSA isolates compared with only [23.3%] of MSSA isolates. Simultaneously, the qacC gene was detected in [63.3%] of MRSA isolates compared to only [13.3 %] of MSSA isolates., with the significant difference between both groups [p <0.05]. We also found an association between the presence of antiseptic resistance genes qacA/B, and increased MIC values to both savlon, hypochlorite, and Phenol [p <0.05]. We also showed that an association between the presence of antiseptic resistance genes qacC and the MICs to both savlon, hypochlorite, and Phenol [p <0.05]. CONCLUSION: Our study detects biocide resistance genes [qacA/B, qacC] in Staphylococcus aureus isolates in Egypt; more studies are needed to correct the biocide uses and to support infection control programs to avoid the development of new resistance mechanisms.Walid SabryMohammed Yosry Al-DakhakhniIbrahim Al-hoseinyRaafat Abd Rabbo Abdel TawabAl-Azhar University, Faculty of Medicine (Damietta)articleresistancebiocidesstaphylococcus aureusmethicillin-resistantbiocides’ resistance genesMedicine (General)R5-920ENInternational Journal of Medical Arts, Vol 3, Iss 3, Pp 1602-1610 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	resistance biocides staphylococcus aureus methicillin-resistant biocides’ resistance genes Medicine (General) R5-920
spellingShingle	resistance biocides staphylococcus aureus methicillin-resistant biocides’ resistance genes Medicine (General) R5-920 Walid Sabry Mohammed Yosry Al-Dakhakhni Ibrahim Al-hoseiny Raafat Abd Rabbo Abdel Tawab Detection of Biocides Resistance Genes among Clinical Isolates of Both Methicillin-Resistant and Methicillin-Sensitive Strains of Staphylococcus Aureus
description	BACKGROUND: Biocides compounds are widely used in infection control programs, including hand washing and skin decolonization before invasive procedures. However, its overuse leads to the emergence of Staphylococcus aureus with decreased biocides susceptibility, which became a problem in hospitals in different parts of the world. AIM OF THE WORK: The present work aims to study the susceptibility of methicillin-resistant isolates of staphylococci aureus, obtained from different clinical samples to commonly used biocides, determines the prevalence of the biocide resistance genes, qacA/B, and qacC, among these isolates. METHODS: Biocide’s resistance genes [qacA/B, qacC] in Both Methicillin-Resistant and Methicillin-Sensitive Strains of Staphylococcus aureus were detected by PCR method. The minimum inhibitory concentrations [MICs] of Biocide agents were determined by broth dilution method, whereas antibiotic susceptibility was analyzed by disk diffusion and MICs methods according to Clinical and Laboratory Standards Institute [CLSI] criteria. RESULTS: The frequency of Biocides resistance genes [qacA/B, qacC] was found to be high in our clinical staphylococci isolates. The qacA/B gene was detected in [63.3%] of MRSA isolates compared with only [23.3%] of MSSA isolates. Simultaneously, the qacC gene was detected in [63.3%] of MRSA isolates compared to only [13.3 %] of MSSA isolates., with the significant difference between both groups [p <0.05]. We also found an association between the presence of antiseptic resistance genes qacA/B, and increased MIC values to both savlon, hypochlorite, and Phenol [p <0.05]. We also showed that an association between the presence of antiseptic resistance genes qacC and the MICs to both savlon, hypochlorite, and Phenol [p <0.05]. CONCLUSION: Our study detects biocide resistance genes [qacA/B, qacC] in Staphylococcus aureus isolates in Egypt; more studies are needed to correct the biocide uses and to support infection control programs to avoid the development of new resistance mechanisms.
format	article
author	Walid Sabry Mohammed Yosry Al-Dakhakhni Ibrahim Al-hoseiny Raafat Abd Rabbo Abdel Tawab
author_facet	Walid Sabry Mohammed Yosry Al-Dakhakhni Ibrahim Al-hoseiny Raafat Abd Rabbo Abdel Tawab
author_sort	Walid Sabry
title	Detection of Biocides Resistance Genes among Clinical Isolates of Both Methicillin-Resistant and Methicillin-Sensitive Strains of Staphylococcus Aureus
title_short	Detection of Biocides Resistance Genes among Clinical Isolates of Both Methicillin-Resistant and Methicillin-Sensitive Strains of Staphylococcus Aureus
title_full	Detection of Biocides Resistance Genes among Clinical Isolates of Both Methicillin-Resistant and Methicillin-Sensitive Strains of Staphylococcus Aureus
title_fullStr	Detection of Biocides Resistance Genes among Clinical Isolates of Both Methicillin-Resistant and Methicillin-Sensitive Strains of Staphylococcus Aureus
title_full_unstemmed	Detection of Biocides Resistance Genes among Clinical Isolates of Both Methicillin-Resistant and Methicillin-Sensitive Strains of Staphylococcus Aureus
title_sort	detection of biocides resistance genes among clinical isolates of both methicillin-resistant and methicillin-sensitive strains of staphylococcus aureus
publisher	Al-Azhar University, Faculty of Medicine (Damietta)
publishDate	2021
url	https://doaj.org/article/8c5a99e3fcba40fba6bf53b02138bcad
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Detection of Biocides Resistance Genes among Clinical Isolates of Both Methicillin-Resistant and Methicillin-Sensitive Strains of Staphylococcus Aureus

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