Effect of Starvation in Reversing Cancer Chemoresistance Based on Drug-Resistance Detection by Dextran Nanoparticles

Chenglong Wang,1 Xuzhu Gao,1 Fanchen Wang,1 Wencai Guan,1 Hongjing Dou,2 Guoxiong Xu1 1Research Center for Clinical Medicine, Jinshan Hospital, Fudan University, Shanghai 201508, People’s Republic of China; 2The State Key Laboratory of Metal Matrix Composites, School of Materials Science a...

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Autores principales:	Wang C, Gao X, Wang F, Guan W, Dou H, Xu G
Formato:	article
Lenguaje:	EN
Publicado:	Dove Medical Press 2020
Materias:	cancer drug-resistance p-glycoprotein starvation therapy nanoparticles resistance reversal Medicine (General) R5-920
Acceso en línea:	https://doaj.org/article/8c5ac6de0f0542238f689453bba6dbdb
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spelling	oai:doaj.org-article:8c5ac6de0f0542238f689453bba6dbdb2021-12-02T12:01:56ZEffect of Starvation in Reversing Cancer Chemoresistance Based on Drug-Resistance Detection by Dextran Nanoparticles1178-2013https://doaj.org/article/8c5ac6de0f0542238f689453bba6dbdb2020-11-01T00:00:00Zhttps://www.dovepress.com/effect-of-starvation-in-reversing-cancer-chemoresistance-based-on-drug-peer-reviewed-article-IJNhttps://doaj.org/toc/1178-2013Chenglong Wang,1 Xuzhu Gao,1 Fanchen Wang,1 Wencai Guan,1 Hongjing Dou,2 Guoxiong Xu1 1Research Center for Clinical Medicine, Jinshan Hospital, Fudan University, Shanghai 201508, People’s Republic of China; 2The State Key Laboratory of Metal Matrix Composites, School of Materials Science and Engineering, Shanghai Jiao Tong University, Shanghai 200240, People’s Republic of ChinaCorrespondence: Guoxiong XuResearch Center for Clinical Medicine, Jinshan Hospital, Fudan University, 1508 Longhang Road, Shanghai 201508, People’s Republic of ChinaTel +86-21-34189990Fax +86-21-57039502Email guoxiong.xu@fudan.edu.cnHongjing DouThe State Key Laboratory of Metal Matrix Composites, School of Materials Science and Engineering, Shanghai Jiao Tong University, 800 Dongchuan Road, Shanghai 200240, People’s Republic of ChinaTel +86-21-34202956Fax +86-21-34202745Email hjdou@sjtu.edu.cnIntroduction: Chemoresistance leads to chemotherapy failure in patients with cancer. Multidrug resistance (MDR) in cancer is mainly caused by the high expression of P-glycoprotein encoded by the MDR1 gene, which is an ATP-dependent protease. Keeping the stronger invasion and migration abilities of chemoresistant cells in cancer also requires more ATP consumption. Herein, we aimed to reverse resistance by reducing the glucose supply in the cellular environment.Methods: A starvation approach in reversing chemoresistance was applied, which was implemented through preparing fluorescent dextran-based nanoparticles to detect the proportion of chemoresistant cells in the chemoresistant/chemosensitive cell mixture after cells cultured in a low-glucose condition.Results: Chemoresistant cells had higher glucose consumption with higher ATPase expression and stronger glucose dependence compared to chemosensitive cells. Moreover, cancer cells cultured in a low-glucose condition reduced the proportion of chemoresistant cells.Conclusion: Starvation therapy can be used as a new method to reverse drug resistance in cancer.Keywords: cancer drug-resistance, P-glycoprotein, starvation therapy, nanoparticles, resistance reversalWang CGao XWang FGuan WDou HXu GDove Medical Pressarticlecancer drug-resistancep-glycoproteinstarvation therapynanoparticlesresistance reversalMedicine (General)R5-920ENInternational Journal of Nanomedicine, Vol Volume 15, Pp 9255-9264 (2020)
institution	DOAJ
collection	DOAJ
language	EN
topic	cancer drug-resistance p-glycoprotein starvation therapy nanoparticles resistance reversal Medicine (General) R5-920
spellingShingle	cancer drug-resistance p-glycoprotein starvation therapy nanoparticles resistance reversal Medicine (General) R5-920 Wang C Gao X Wang F Guan W Dou H Xu G Effect of Starvation in Reversing Cancer Chemoresistance Based on Drug-Resistance Detection by Dextran Nanoparticles
description	Chenglong Wang,1 Xuzhu Gao,1 Fanchen Wang,1 Wencai Guan,1 Hongjing Dou,2 Guoxiong Xu1 1Research Center for Clinical Medicine, Jinshan Hospital, Fudan University, Shanghai 201508, People’s Republic of China; 2The State Key Laboratory of Metal Matrix Composites, School of Materials Science and Engineering, Shanghai Jiao Tong University, Shanghai 200240, People’s Republic of ChinaCorrespondence: Guoxiong XuResearch Center for Clinical Medicine, Jinshan Hospital, Fudan University, 1508 Longhang Road, Shanghai 201508, People’s Republic of ChinaTel +86-21-34189990Fax +86-21-57039502Email guoxiong.xu@fudan.edu.cnHongjing DouThe State Key Laboratory of Metal Matrix Composites, School of Materials Science and Engineering, Shanghai Jiao Tong University, 800 Dongchuan Road, Shanghai 200240, People’s Republic of ChinaTel +86-21-34202956Fax +86-21-34202745Email hjdou@sjtu.edu.cnIntroduction: Chemoresistance leads to chemotherapy failure in patients with cancer. Multidrug resistance (MDR) in cancer is mainly caused by the high expression of P-glycoprotein encoded by the MDR1 gene, which is an ATP-dependent protease. Keeping the stronger invasion and migration abilities of chemoresistant cells in cancer also requires more ATP consumption. Herein, we aimed to reverse resistance by reducing the glucose supply in the cellular environment.Methods: A starvation approach in reversing chemoresistance was applied, which was implemented through preparing fluorescent dextran-based nanoparticles to detect the proportion of chemoresistant cells in the chemoresistant/chemosensitive cell mixture after cells cultured in a low-glucose condition.Results: Chemoresistant cells had higher glucose consumption with higher ATPase expression and stronger glucose dependence compared to chemosensitive cells. Moreover, cancer cells cultured in a low-glucose condition reduced the proportion of chemoresistant cells.Conclusion: Starvation therapy can be used as a new method to reverse drug resistance in cancer.Keywords: cancer drug-resistance, P-glycoprotein, starvation therapy, nanoparticles, resistance reversal
format	article
author	Wang C Gao X Wang F Guan W Dou H Xu G
author_facet	Wang C Gao X Wang F Guan W Dou H Xu G
author_sort	Wang C
title	Effect of Starvation in Reversing Cancer Chemoresistance Based on Drug-Resistance Detection by Dextran Nanoparticles
title_short	Effect of Starvation in Reversing Cancer Chemoresistance Based on Drug-Resistance Detection by Dextran Nanoparticles
title_full	Effect of Starvation in Reversing Cancer Chemoresistance Based on Drug-Resistance Detection by Dextran Nanoparticles
title_fullStr	Effect of Starvation in Reversing Cancer Chemoresistance Based on Drug-Resistance Detection by Dextran Nanoparticles
title_full_unstemmed	Effect of Starvation in Reversing Cancer Chemoresistance Based on Drug-Resistance Detection by Dextran Nanoparticles
title_sort	effect of starvation in reversing cancer chemoresistance based on drug-resistance detection by dextran nanoparticles
publisher	Dove Medical Press
publishDate	2020
url	https://doaj.org/article/8c5ac6de0f0542238f689453bba6dbdb
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Effect of Starvation in Reversing Cancer Chemoresistance Based on Drug-Resistance Detection by Dextran Nanoparticles

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