Green Process: Improved Semi-Continuous Fermentation of Pichia pastoris Based on the Principle of Vitality Cell Separation

The large-scale fermentation of Pichia pastoris for recombinant protein production would be time consuming and produce a large amount of waste yeast. Here we introduce a novel semi-continuous fermentation process for P. pastoris GS115 that can separate vitality cells from broth and recycle the cells...

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Autores principales:	Denggang Wang, Wenjie Li, Xinying Zhang, Shuli Liang, Ying Lin
Formato:	article
Lenguaje:	EN
Publicado:	Frontiers Media S.A. 2021
Materias:	semi-continuous cultivation pichia pastoris sedimentation vitality cells metal ion Biotechnology TP248.13-248.65
Acceso en línea:	https://doaj.org/article/8c5b9bc7f3fc4f9aadb84f6d7e5af969
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spelling	oai:doaj.org-article:8c5b9bc7f3fc4f9aadb84f6d7e5af9692021-12-01T17:57:24ZGreen Process: Improved Semi-Continuous Fermentation of Pichia pastoris Based on the Principle of Vitality Cell Separation2296-418510.3389/fbioe.2021.777774https://doaj.org/article/8c5b9bc7f3fc4f9aadb84f6d7e5af9692021-11-01T00:00:00Zhttps://www.frontiersin.org/articles/10.3389/fbioe.2021.777774/fullhttps://doaj.org/toc/2296-4185The large-scale fermentation of Pichia pastoris for recombinant protein production would be time consuming and produce a large amount of waste yeast. Here we introduce a novel semi-continuous fermentation process for P. pastoris GS115 that can separate vitality cells from broth and recycle the cells to produce high-secretory recombinant pectate lyase. It is based on differences in cell sedimentation coefficients with the formation of salt bridges between metal ions and various cell states. Compared to batch-fed cultivation and general semi-continuous culture, the novel process has significant advantages, such as consuming fewer resources, taking less time, and producing less waste yeast. Sedimentation with the addition of Fe3+ metal ions consumed 14.8 ± 0.0% glycerol, 97.8 ± 1.3% methanol, 55.0 ± 0.9 inorganic salts, 81.5 ± 0.0% time cost, and 77.0 ± 0.1% waste yeast versus batch-fed cultivation to produce an equal amount of protein; in addition, the cost of solid–liquid separation was lower for cells in the collected fermentation broth. The process is economically and environmentally efficient for producing recombinant proteins.Denggang WangWenjie LiXinying ZhangShuli LiangYing LinFrontiers Media S.A.articlesemi-continuous cultivationpichia pastorissedimentationvitality cellsmetal ionBiotechnologyTP248.13-248.65ENFrontiers in Bioengineering and Biotechnology, Vol 9 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	semi-continuous cultivation pichia pastoris sedimentation vitality cells metal ion Biotechnology TP248.13-248.65
spellingShingle	semi-continuous cultivation pichia pastoris sedimentation vitality cells metal ion Biotechnology TP248.13-248.65 Denggang Wang Wenjie Li Xinying Zhang Shuli Liang Ying Lin Green Process: Improved Semi-Continuous Fermentation of Pichia pastoris Based on the Principle of Vitality Cell Separation
description	The large-scale fermentation of Pichia pastoris for recombinant protein production would be time consuming and produce a large amount of waste yeast. Here we introduce a novel semi-continuous fermentation process for P. pastoris GS115 that can separate vitality cells from broth and recycle the cells to produce high-secretory recombinant pectate lyase. It is based on differences in cell sedimentation coefficients with the formation of salt bridges between metal ions and various cell states. Compared to batch-fed cultivation and general semi-continuous culture, the novel process has significant advantages, such as consuming fewer resources, taking less time, and producing less waste yeast. Sedimentation with the addition of Fe3+ metal ions consumed 14.8 ± 0.0% glycerol, 97.8 ± 1.3% methanol, 55.0 ± 0.9 inorganic salts, 81.5 ± 0.0% time cost, and 77.0 ± 0.1% waste yeast versus batch-fed cultivation to produce an equal amount of protein; in addition, the cost of solid–liquid separation was lower for cells in the collected fermentation broth. The process is economically and environmentally efficient for producing recombinant proteins.
format	article
author	Denggang Wang Wenjie Li Xinying Zhang Shuli Liang Ying Lin
author_facet	Denggang Wang Wenjie Li Xinying Zhang Shuli Liang Ying Lin
author_sort	Denggang Wang
title	Green Process: Improved Semi-Continuous Fermentation of Pichia pastoris Based on the Principle of Vitality Cell Separation
title_short	Green Process: Improved Semi-Continuous Fermentation of Pichia pastoris Based on the Principle of Vitality Cell Separation
title_full	Green Process: Improved Semi-Continuous Fermentation of Pichia pastoris Based on the Principle of Vitality Cell Separation
title_fullStr	Green Process: Improved Semi-Continuous Fermentation of Pichia pastoris Based on the Principle of Vitality Cell Separation
title_full_unstemmed	Green Process: Improved Semi-Continuous Fermentation of Pichia pastoris Based on the Principle of Vitality Cell Separation
title_sort	green process: improved semi-continuous fermentation of pichia pastoris based on the principle of vitality cell separation
publisher	Frontiers Media S.A.
publishDate	2021
url	https://doaj.org/article/8c5b9bc7f3fc4f9aadb84f6d7e5af969
work_keys_str_mv	AT denggangwang greenprocessimprovedsemicontinuousfermentationofpichiapastorisbasedontheprincipleofvitalitycellseparation AT wenjieli greenprocessimprovedsemicontinuousfermentationofpichiapastorisbasedontheprincipleofvitalitycellseparation AT xinyingzhang greenprocessimprovedsemicontinuousfermentationofpichiapastorisbasedontheprincipleofvitalitycellseparation AT shuliliang greenprocessimprovedsemicontinuousfermentationofpichiapastorisbasedontheprincipleofvitalitycellseparation AT yinglin greenprocessimprovedsemicontinuousfermentationofpichiapastorisbasedontheprincipleofvitalitycellseparation
_version_	1718404762619084800

Green Process: Improved Semi-Continuous Fermentation of Pichia pastoris Based on the Principle of Vitality Cell Separation

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