Nanoencapsulation of sulforaphane in broccoli membrane vesicles and their in vitro antiproliferative activity

Context The development of nanocarriers of plant origin, such as plant cell membranes, has recently been investigated. Also, plant bioactive compounds as sulforaphane (SFN) from broccoli have recognized antioxidant or anticancer properties. Objective To investigate the capacity of membrane vesicles...

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Autores principales:	Lucía Yepes-Molina, Micaela Carvajal
Formato:	article
Lenguaje:	EN
Publicado:	Taylor & Francis Group 2021
Materias:	anticancer activity brassicas encapsulation isothiocyanates melanoma nanocarrier Therapeutics. Pharmacology RM1-950
Acceso en línea:	https://doaj.org/article/8c5dc963fe4b44d68405b0322f1ee592
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spelling	oai:doaj.org-article:8c5dc963fe4b44d68405b0322f1ee5922021-11-04T15:00:42ZNanoencapsulation of sulforaphane in broccoli membrane vesicles and their in vitro antiproliferative activity1388-02091744-511610.1080/13880209.2021.1992450https://doaj.org/article/8c5dc963fe4b44d68405b0322f1ee5922021-01-01T00:00:00Zhttp://dx.doi.org/10.1080/13880209.2021.1992450https://doaj.org/toc/1388-0209https://doaj.org/toc/1744-5116Context The development of nanocarriers of plant origin, such as plant cell membranes, has recently been investigated. Also, plant bioactive compounds as sulforaphane (SFN) from broccoli have recognized antioxidant or anticancer properties. Objective To investigate the capacity of membrane vesicles from broccoli (BM-vesicles) to encapsulate SFN and their application in the cancer cell line. Materials and methods Physicochemical analysis was carried out to characterize BM-vesicles through different approaches: dynamic light scattering, transmission electron microscopy, stopped-flow analysis, and proteomic analysis. They were applied at different concentrations (BM-vesicles at 0.04–0.00315% of protein and SFN at 5, 25, and 100 µM) in SK-MEL-28 cells during 24 h for studying cytotoxicity and gene expression. Results The entrapment efficiency was 41%. The anticancer activity tested in cells showed a decrease in proliferation when SFN in BM-vesicles was utilized. Expression patterns when SFN was applied in an encapsulated form showed a reduction of cancer markers and an increase of AQP3. Also, the metabolism of SFN occurred inside of cells, and higher SFN penetrated when it was encapsulated. Discussion The results showed that encapsulated SFN was better absorbed by melanoma cells providing metabolism products and a reduction of cancer molecular markers. Also aquaporin, AQP3 was pointed to as an important marker since it appeared to play a key role in homeostasis due to the importance of water transport in biological processes. Conclusion These results indicate that SFN and SFN encapsulated in BM-vesicles have a high activity for the inhibition of melanocyte development. Therefore, BM-vesicles could serve as nanocarriers for drugs.Lucía Yepes-MolinaMicaela CarvajalTaylor & Francis Grouparticleanticancer activitybrassicasencapsulationisothiocyanatesmelanomananocarrierTherapeutics. PharmacologyRM1-950ENPharmaceutical Biology, Vol 59, Iss 1, Pp 1490-1504 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	anticancer activity brassicas encapsulation isothiocyanates melanoma nanocarrier Therapeutics. Pharmacology RM1-950
spellingShingle	anticancer activity brassicas encapsulation isothiocyanates melanoma nanocarrier Therapeutics. Pharmacology RM1-950 Lucía Yepes-Molina Micaela Carvajal Nanoencapsulation of sulforaphane in broccoli membrane vesicles and their in vitro antiproliferative activity
description	Context The development of nanocarriers of plant origin, such as plant cell membranes, has recently been investigated. Also, plant bioactive compounds as sulforaphane (SFN) from broccoli have recognized antioxidant or anticancer properties. Objective To investigate the capacity of membrane vesicles from broccoli (BM-vesicles) to encapsulate SFN and their application in the cancer cell line. Materials and methods Physicochemical analysis was carried out to characterize BM-vesicles through different approaches: dynamic light scattering, transmission electron microscopy, stopped-flow analysis, and proteomic analysis. They were applied at different concentrations (BM-vesicles at 0.04–0.00315% of protein and SFN at 5, 25, and 100 µM) in SK-MEL-28 cells during 24 h for studying cytotoxicity and gene expression. Results The entrapment efficiency was 41%. The anticancer activity tested in cells showed a decrease in proliferation when SFN in BM-vesicles was utilized. Expression patterns when SFN was applied in an encapsulated form showed a reduction of cancer markers and an increase of AQP3. Also, the metabolism of SFN occurred inside of cells, and higher SFN penetrated when it was encapsulated. Discussion The results showed that encapsulated SFN was better absorbed by melanoma cells providing metabolism products and a reduction of cancer molecular markers. Also aquaporin, AQP3 was pointed to as an important marker since it appeared to play a key role in homeostasis due to the importance of water transport in biological processes. Conclusion These results indicate that SFN and SFN encapsulated in BM-vesicles have a high activity for the inhibition of melanocyte development. Therefore, BM-vesicles could serve as nanocarriers for drugs.
format	article
author	Lucía Yepes-Molina Micaela Carvajal
author_facet	Lucía Yepes-Molina Micaela Carvajal
author_sort	Lucía Yepes-Molina
title	Nanoencapsulation of sulforaphane in broccoli membrane vesicles and their in vitro antiproliferative activity
title_short	Nanoencapsulation of sulforaphane in broccoli membrane vesicles and their in vitro antiproliferative activity
title_full	Nanoencapsulation of sulforaphane in broccoli membrane vesicles and their in vitro antiproliferative activity
title_fullStr	Nanoencapsulation of sulforaphane in broccoli membrane vesicles and their in vitro antiproliferative activity
title_full_unstemmed	Nanoencapsulation of sulforaphane in broccoli membrane vesicles and their in vitro antiproliferative activity
title_sort	nanoencapsulation of sulforaphane in broccoli membrane vesicles and their in vitro antiproliferative activity
publisher	Taylor & Francis Group
publishDate	2021
url	https://doaj.org/article/8c5dc963fe4b44d68405b0322f1ee592
work_keys_str_mv	AT luciayepesmolina nanoencapsulationofsulforaphaneinbroccolimembranevesiclesandtheirinvitroantiproliferativeactivity AT micaelacarvajal nanoencapsulationofsulforaphaneinbroccolimembranevesiclesandtheirinvitroantiproliferativeactivity
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Nanoencapsulation of sulforaphane in broccoli membrane vesicles and their in vitro antiproliferative activity

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