HATMSC Secreted Factors in the Hydrogel as a Potential Treatment for Chronic Wounds—In Vitro Study

Mesenchymal stem cells (MSCs) can improve chronic wound healing; however, recent studies suggest that the therapeutic effect of MSCs is mediated mainly through the growth factors and cytokines secreted by these cells, referred to as the MSC secretome. To overcome difficulties related to the translat...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Honorata Kraskiewicz, Piotr Hinc, Agnieszka Krawczenko, Aleksandra Bielawska-Pohl, Maria Paprocka, Danuta Witkowska, Isma Liza Mohd Isa, Abhay Pandit, Aleksandra Klimczak
Formato:	article
Lenguaje:	EN
Publicado:	MDPI AG 2021
Materias:	adipose tissue-derived mesenchymal stem cells mesenchymal stem cell secretome MSC secretome chronic wound collagen hydrogel Biology (General) QH301-705.5 Chemistry QD1-999
Acceso en línea:	https://doaj.org/article/8c5de3bcc4694b87b5009aff79351175
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:8c5de3bcc4694b87b5009aff79351175
record_format	dspace
spelling	oai:doaj.org-article:8c5de3bcc4694b87b5009aff793511752021-11-25T17:54:37ZHATMSC Secreted Factors in the Hydrogel as a Potential Treatment for Chronic Wounds—In Vitro Study10.3390/ijms2222122411422-00671661-6596https://doaj.org/article/8c5de3bcc4694b87b5009aff793511752021-11-01T00:00:00Zhttps://www.mdpi.com/1422-0067/22/22/12241https://doaj.org/toc/1661-6596https://doaj.org/toc/1422-0067Mesenchymal stem cells (MSCs) can improve chronic wound healing; however, recent studies suggest that the therapeutic effect of MSCs is mediated mainly through the growth factors and cytokines secreted by these cells, referred to as the MSC secretome. To overcome difficulties related to the translation of cell therapy into clinical use such as efficacy, safety and cost, we propose a hydrogel loaded with a secretome from the recently established human adipose tissue mesenchymal stem cell line (HATMSC2) as a potential treatment for chronic wounds. Biocompatibility and biological activity of hydrogel-released HATMSC2 supernatant were investigated in vitro by assessing the proliferation and metabolic activity of human fibroblast, endothelial cells and keratinocytes. Hydrogel degradation was measured using hydroxyproline assay while protein released from the hydrogel was assessed by interleukin-8 (IL-8) and macrophage chemoattractant protein-1 (MCP-1) ELISAs. Pro-angiogenic activity of the developed treatment was assessed by tube formation assay while the presence of pro-angiogenic miRNAs in the HATMSC2 supernatant was investigated using real-time RT-PCR. The results demonstrated that the therapeutic effect of the HATMSC2-produced factors is maintained following incorporation into collagen hydrogel as confirmed by increased proliferation of skin-origin cells and improved angiogenic properties of endothelial cells. In addition, HATMSC2 supernatant revealed antimicrobial activity, and which therefore, in combination with the hydrogel has a potential to be used as advanced wound-healing dressing.Honorata KraskiewiczPiotr HincAgnieszka KrawczenkoAleksandra Bielawska-PohlMaria PaprockaDanuta WitkowskaIsma Liza Mohd IsaAbhay PanditAleksandra KlimczakMDPI AGarticleadipose tissue-derived mesenchymal stem cellsmesenchymal stem cell secretomeMSC secretomechronic woundcollagen hydrogelBiology (General)QH301-705.5ChemistryQD1-999ENInternational Journal of Molecular Sciences, Vol 22, Iss 12241, p 12241 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	adipose tissue-derived mesenchymal stem cells mesenchymal stem cell secretome MSC secretome chronic wound collagen hydrogel Biology (General) QH301-705.5 Chemistry QD1-999
spellingShingle	adipose tissue-derived mesenchymal stem cells mesenchymal stem cell secretome MSC secretome chronic wound collagen hydrogel Biology (General) QH301-705.5 Chemistry QD1-999 Honorata Kraskiewicz Piotr Hinc Agnieszka Krawczenko Aleksandra Bielawska-Pohl Maria Paprocka Danuta Witkowska Isma Liza Mohd Isa Abhay Pandit Aleksandra Klimczak HATMSC Secreted Factors in the Hydrogel as a Potential Treatment for Chronic Wounds—In Vitro Study
description	Mesenchymal stem cells (MSCs) can improve chronic wound healing; however, recent studies suggest that the therapeutic effect of MSCs is mediated mainly through the growth factors and cytokines secreted by these cells, referred to as the MSC secretome. To overcome difficulties related to the translation of cell therapy into clinical use such as efficacy, safety and cost, we propose a hydrogel loaded with a secretome from the recently established human adipose tissue mesenchymal stem cell line (HATMSC2) as a potential treatment for chronic wounds. Biocompatibility and biological activity of hydrogel-released HATMSC2 supernatant were investigated in vitro by assessing the proliferation and metabolic activity of human fibroblast, endothelial cells and keratinocytes. Hydrogel degradation was measured using hydroxyproline assay while protein released from the hydrogel was assessed by interleukin-8 (IL-8) and macrophage chemoattractant protein-1 (MCP-1) ELISAs. Pro-angiogenic activity of the developed treatment was assessed by tube formation assay while the presence of pro-angiogenic miRNAs in the HATMSC2 supernatant was investigated using real-time RT-PCR. The results demonstrated that the therapeutic effect of the HATMSC2-produced factors is maintained following incorporation into collagen hydrogel as confirmed by increased proliferation of skin-origin cells and improved angiogenic properties of endothelial cells. In addition, HATMSC2 supernatant revealed antimicrobial activity, and which therefore, in combination with the hydrogel has a potential to be used as advanced wound-healing dressing.
format	article
author	Honorata Kraskiewicz Piotr Hinc Agnieszka Krawczenko Aleksandra Bielawska-Pohl Maria Paprocka Danuta Witkowska Isma Liza Mohd Isa Abhay Pandit Aleksandra Klimczak
author_facet	Honorata Kraskiewicz Piotr Hinc Agnieszka Krawczenko Aleksandra Bielawska-Pohl Maria Paprocka Danuta Witkowska Isma Liza Mohd Isa Abhay Pandit Aleksandra Klimczak
author_sort	Honorata Kraskiewicz
title	HATMSC Secreted Factors in the Hydrogel as a Potential Treatment for Chronic Wounds—In Vitro Study
title_short	HATMSC Secreted Factors in the Hydrogel as a Potential Treatment for Chronic Wounds—In Vitro Study
title_full	HATMSC Secreted Factors in the Hydrogel as a Potential Treatment for Chronic Wounds—In Vitro Study
title_fullStr	HATMSC Secreted Factors in the Hydrogel as a Potential Treatment for Chronic Wounds—In Vitro Study
title_full_unstemmed	HATMSC Secreted Factors in the Hydrogel as a Potential Treatment for Chronic Wounds—In Vitro Study
title_sort	hatmsc secreted factors in the hydrogel as a potential treatment for chronic wounds—in vitro study
publisher	MDPI AG
publishDate	2021
url	https://doaj.org/article/8c5de3bcc4694b87b5009aff79351175
work_keys_str_mv	AT honoratakraskiewicz hatmscsecretedfactorsinthehydrogelasapotentialtreatmentforchronicwoundsinvitrostudy AT piotrhinc hatmscsecretedfactorsinthehydrogelasapotentialtreatmentforchronicwoundsinvitrostudy AT agnieszkakrawczenko hatmscsecretedfactorsinthehydrogelasapotentialtreatmentforchronicwoundsinvitrostudy AT aleksandrabielawskapohl hatmscsecretedfactorsinthehydrogelasapotentialtreatmentforchronicwoundsinvitrostudy AT mariapaprocka hatmscsecretedfactorsinthehydrogelasapotentialtreatmentforchronicwoundsinvitrostudy AT danutawitkowska hatmscsecretedfactorsinthehydrogelasapotentialtreatmentforchronicwoundsinvitrostudy AT ismalizamohdisa hatmscsecretedfactorsinthehydrogelasapotentialtreatmentforchronicwoundsinvitrostudy AT abhaypandit hatmscsecretedfactorsinthehydrogelasapotentialtreatmentforchronicwoundsinvitrostudy AT aleksandraklimczak hatmscsecretedfactorsinthehydrogelasapotentialtreatmentforchronicwoundsinvitrostudy
_version_	1718411873883258880

HATMSC Secreted Factors in the Hydrogel as a Potential Treatment for Chronic Wounds—In Vitro Study

Ejemplares similares