Oxygen atoms are critical in rendering THP-1 leukaemia cells susceptible to cold physical plasma-induced apoptosis

Abstract Cold physical plasma has been suggested as a powerful new tool in oncology. However, some cancer cells such as THP-1 leukaemia cells have been shown to be resistant towards plasma-induced cell death, thereby serving as a good model for optimizing plasmas in order to foster pro-apoptotic ant...

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Autores principales:	Sander Bekeschus, Kristian Wende, Mohamed Mokhtar Hefny, Katrin Rödder, Helena Jablonowski, Anke Schmidt, Thomas von Woedtke, Klaus-Dieter Weltmann, Jan Benedikt
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2017
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/8c629fc8ae7f40a389850a8c3b3db68d
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spelling	oai:doaj.org-article:8c629fc8ae7f40a389850a8c3b3db68d2021-12-02T12:30:52ZOxygen atoms are critical in rendering THP-1 leukaemia cells susceptible to cold physical plasma-induced apoptosis10.1038/s41598-017-03131-y2045-2322https://doaj.org/article/8c629fc8ae7f40a389850a8c3b3db68d2017-06-01T00:00:00Zhttps://doi.org/10.1038/s41598-017-03131-yhttps://doaj.org/toc/2045-2322Abstract Cold physical plasma has been suggested as a powerful new tool in oncology. However, some cancer cells such as THP-1 leukaemia cells have been shown to be resistant towards plasma-induced cell death, thereby serving as a good model for optimizing plasmas in order to foster pro-apoptotic anticancer effects. A helium/oxygen radio frequency driven atmospheric plasma profoundly induced apoptosis in THP-1 cells whereas helium, humidified helium, and humidified helium/oxygen plasmas were inefficient. Hydrogen peroxide – previously shown as central plasma-derived agent – did not participate in the killing reaction but our results suggest hypochlorous acid to be responsible for the effect observed. Proteomic analysis of THP-1 cells exposed to He/O2 plasma emphasized a prominent growth retardation, cell stress, apoptosis, and a pro-immunogenic profile. Altogether, a plasma setting that inactivates previously unresponsive leukaemia cells is presented. Crucial reactive species in the plasma and liquid environment were identified and discussed, deciphering the complexity of plasma from the gas phase into the liquid down to the cellular response mechanism. These results may help tailoring plasmas for clinical applications such as oxidation-insensitive types of cancer.Sander BekeschusKristian WendeMohamed Mokhtar HefnyKatrin RödderHelena JablonowskiAnke SchmidtThomas von WoedtkeKlaus-Dieter WeltmannJan BenediktNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 7, Iss 1, Pp 1-12 (2017)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Sander Bekeschus Kristian Wende Mohamed Mokhtar Hefny Katrin Rödder Helena Jablonowski Anke Schmidt Thomas von Woedtke Klaus-Dieter Weltmann Jan Benedikt Oxygen atoms are critical in rendering THP-1 leukaemia cells susceptible to cold physical plasma-induced apoptosis
description	Abstract Cold physical plasma has been suggested as a powerful new tool in oncology. However, some cancer cells such as THP-1 leukaemia cells have been shown to be resistant towards plasma-induced cell death, thereby serving as a good model for optimizing plasmas in order to foster pro-apoptotic anticancer effects. A helium/oxygen radio frequency driven atmospheric plasma profoundly induced apoptosis in THP-1 cells whereas helium, humidified helium, and humidified helium/oxygen plasmas were inefficient. Hydrogen peroxide – previously shown as central plasma-derived agent – did not participate in the killing reaction but our results suggest hypochlorous acid to be responsible for the effect observed. Proteomic analysis of THP-1 cells exposed to He/O2 plasma emphasized a prominent growth retardation, cell stress, apoptosis, and a pro-immunogenic profile. Altogether, a plasma setting that inactivates previously unresponsive leukaemia cells is presented. Crucial reactive species in the plasma and liquid environment were identified and discussed, deciphering the complexity of plasma from the gas phase into the liquid down to the cellular response mechanism. These results may help tailoring plasmas for clinical applications such as oxidation-insensitive types of cancer.
format	article
author	Sander Bekeschus Kristian Wende Mohamed Mokhtar Hefny Katrin Rödder Helena Jablonowski Anke Schmidt Thomas von Woedtke Klaus-Dieter Weltmann Jan Benedikt
author_facet	Sander Bekeschus Kristian Wende Mohamed Mokhtar Hefny Katrin Rödder Helena Jablonowski Anke Schmidt Thomas von Woedtke Klaus-Dieter Weltmann Jan Benedikt
author_sort	Sander Bekeschus
title	Oxygen atoms are critical in rendering THP-1 leukaemia cells susceptible to cold physical plasma-induced apoptosis
title_short	Oxygen atoms are critical in rendering THP-1 leukaemia cells susceptible to cold physical plasma-induced apoptosis
title_full	Oxygen atoms are critical in rendering THP-1 leukaemia cells susceptible to cold physical plasma-induced apoptosis
title_fullStr	Oxygen atoms are critical in rendering THP-1 leukaemia cells susceptible to cold physical plasma-induced apoptosis
title_full_unstemmed	Oxygen atoms are critical in rendering THP-1 leukaemia cells susceptible to cold physical plasma-induced apoptosis
title_sort	oxygen atoms are critical in rendering thp-1 leukaemia cells susceptible to cold physical plasma-induced apoptosis
publisher	Nature Portfolio
publishDate	2017
url	https://doaj.org/article/8c629fc8ae7f40a389850a8c3b3db68d
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Oxygen atoms are critical in rendering THP-1 leukaemia cells susceptible to cold physical plasma-induced apoptosis

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