Differential gene expression analyses of ten defence response genes during Fusarium wilt infection in resistant and susceptible pigeonpea cultivars

The present study was undertaken to partly understand the molecular mechanism of defence response during pigeonpea-Fusarium udum interaction. To study the response of defence related gene expression using reverse transcription quantitative real time PCR (RT-qPCR), roots of resistant and susceptible...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Vishal R. Patil, Ramesh M. Patel, Vipulkumar B. Parekh, Jayesh Pathak, Gautam Saripalli
Formato:	article
Lenguaje:	EN
Publicado:	Elsevier 2021
Materias:	Pigeonpea Fusarium udum RT-qPCR Defence gene expression Roots Plant ecology QK900-989
Acceso en línea:	https://doaj.org/article/8c641ba97127426b8f342ca4e38b8c86
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:8c641ba97127426b8f342ca4e38b8c86
record_format	dspace
spelling	oai:doaj.org-article:8c641ba97127426b8f342ca4e38b8c862021-12-04T04:36:41ZDifferential gene expression analyses of ten defence response genes during Fusarium wilt infection in resistant and susceptible pigeonpea cultivars2667-064X10.1016/j.stress.2021.100043https://doaj.org/article/8c641ba97127426b8f342ca4e38b8c862021-12-01T00:00:00Zhttp://www.sciencedirect.com/science/article/pii/S2667064X21000427https://doaj.org/toc/2667-064XThe present study was undertaken to partly understand the molecular mechanism of defence response during pigeonpea-Fusarium udum interaction. To study the response of defence related gene expression using reverse transcription quantitative real time PCR (RT-qPCR), roots of resistant and susceptible pigeonpea cultivars were infected with F. udum pathogen and samples were collected at 2, 24, 48, and 72 h after inoculation (HAI). PR2, PR10, PAL2, CHS8, IFS1, F3H, LOX, ADC, SPDS, and SPMS were amongst the ten defence response genes studied. The major changes observed in gene expression for all the ten genes are as follows: (i) Out of the ten genes, the expression of three genes, PR2, PR10, and CHS8, peaked at 48 HAI in resistant cultivars compared to susceptible cultivars (ii) the expression of two genes, IFS1 and F3H, was highly induced at all stages in resistant cultivars, while some genotypic differences were observed in susceptible cultivars (iii) the expression of the LOX gene increased only during the early stages at 2 HAI but decreased at later stages in resistant cultivars (iv) In resistant cultivars, the expression of three genes involved in polyamine synthesis, namely ADC, SPDS, and SPMS, peaked at 24 HAI compared to susceptible cultivars. Overall, increased gene expression was found mostly at earlier stages of infection, indicating that these genes are important in providing early resistance to F. udum infection in pigeon pea cultivars. The LOX gene, which codes for the lipooxygenase enzyme, appears to play a role in conferring susceptibility to pigeonea cultivars against F. udum infection, though more research is needed. Further validation studies of all ten genes using overexpression or RNAi technology will provide more information about their roles in the pigeonpea-F.udum interaction.Vishal R. PatilRamesh M. PatelVipulkumar B. ParekhJayesh PathakGautam SaripalliElsevierarticlePigeonpeaFusarium udumRT-qPCRDefence gene expressionRootsPlant ecologyQK900-989ENPlant Stress, Vol 2, Iss , Pp 100043- (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Pigeonpea Fusarium udum RT-qPCR Defence gene expression Roots Plant ecology QK900-989
spellingShingle	Pigeonpea Fusarium udum RT-qPCR Defence gene expression Roots Plant ecology QK900-989 Vishal R. Patil Ramesh M. Patel Vipulkumar B. Parekh Jayesh Pathak Gautam Saripalli Differential gene expression analyses of ten defence response genes during Fusarium wilt infection in resistant and susceptible pigeonpea cultivars
description	The present study was undertaken to partly understand the molecular mechanism of defence response during pigeonpea-Fusarium udum interaction. To study the response of defence related gene expression using reverse transcription quantitative real time PCR (RT-qPCR), roots of resistant and susceptible pigeonpea cultivars were infected with F. udum pathogen and samples were collected at 2, 24, 48, and 72 h after inoculation (HAI). PR2, PR10, PAL2, CHS8, IFS1, F3H, LOX, ADC, SPDS, and SPMS were amongst the ten defence response genes studied. The major changes observed in gene expression for all the ten genes are as follows: (i) Out of the ten genes, the expression of three genes, PR2, PR10, and CHS8, peaked at 48 HAI in resistant cultivars compared to susceptible cultivars (ii) the expression of two genes, IFS1 and F3H, was highly induced at all stages in resistant cultivars, while some genotypic differences were observed in susceptible cultivars (iii) the expression of the LOX gene increased only during the early stages at 2 HAI but decreased at later stages in resistant cultivars (iv) In resistant cultivars, the expression of three genes involved in polyamine synthesis, namely ADC, SPDS, and SPMS, peaked at 24 HAI compared to susceptible cultivars. Overall, increased gene expression was found mostly at earlier stages of infection, indicating that these genes are important in providing early resistance to F. udum infection in pigeon pea cultivars. The LOX gene, which codes for the lipooxygenase enzyme, appears to play a role in conferring susceptibility to pigeonea cultivars against F. udum infection, though more research is needed. Further validation studies of all ten genes using overexpression or RNAi technology will provide more information about their roles in the pigeonpea-F.udum interaction.
format	article
author	Vishal R. Patil Ramesh M. Patel Vipulkumar B. Parekh Jayesh Pathak Gautam Saripalli
author_facet	Vishal R. Patil Ramesh M. Patel Vipulkumar B. Parekh Jayesh Pathak Gautam Saripalli
author_sort	Vishal R. Patil
title	Differential gene expression analyses of ten defence response genes during Fusarium wilt infection in resistant and susceptible pigeonpea cultivars
title_short	Differential gene expression analyses of ten defence response genes during Fusarium wilt infection in resistant and susceptible pigeonpea cultivars
title_full	Differential gene expression analyses of ten defence response genes during Fusarium wilt infection in resistant and susceptible pigeonpea cultivars
title_fullStr	Differential gene expression analyses of ten defence response genes during Fusarium wilt infection in resistant and susceptible pigeonpea cultivars
title_full_unstemmed	Differential gene expression analyses of ten defence response genes during Fusarium wilt infection in resistant and susceptible pigeonpea cultivars
title_sort	differential gene expression analyses of ten defence response genes during fusarium wilt infection in resistant and susceptible pigeonpea cultivars
publisher	Elsevier
publishDate	2021
url	https://doaj.org/article/8c641ba97127426b8f342ca4e38b8c86
work_keys_str_mv	AT vishalrpatil differentialgeneexpressionanalysesoftendefenceresponsegenesduringfusariumwiltinfectioninresistantandsusceptiblepigeonpeacultivars AT rameshmpatel differentialgeneexpressionanalysesoftendefenceresponsegenesduringfusariumwiltinfectioninresistantandsusceptiblepigeonpeacultivars AT vipulkumarbparekh differentialgeneexpressionanalysesoftendefenceresponsegenesduringfusariumwiltinfectioninresistantandsusceptiblepigeonpeacultivars AT jayeshpathak differentialgeneexpressionanalysesoftendefenceresponsegenesduringfusariumwiltinfectioninresistantandsusceptiblepigeonpeacultivars AT gautamsaripalli differentialgeneexpressionanalysesoftendefenceresponsegenesduringfusariumwiltinfectioninresistantandsusceptiblepigeonpeacultivars
_version_	1718372926377426944

Differential gene expression analyses of ten defence response genes during Fusarium wilt infection in resistant and susceptible pigeonpea cultivars

Ejemplares similares