Analyzing the Metabolomic Profile of Yellowtail (<i>Seriola quinquerdiata</i>) by Capillary Electrophoresis–Time of Flight Mass Spectrometry to Determine Geographical Origin

Country-of-origin violations have occurred in which some merchants have fraudulently sold cheap Japanese yellowtail (<i>Seriola quinqueradiata</i>) by presenting them as domestic Korean products. There are many methods for determining the origins of marine organisms, such as molecular ge...

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Autores principales:	Jiyoung Shin, Junho Yang, Eunji Cha, Hyunsuk Kim, Yoonhyeung Lee, Soi Kim, Iseul Choi, Jiyoung Yang
Formato:	article
Lenguaje:	EN
Publicado:	MDPI AG 2021
Materias:	yellowtail metabolomics geographical origin discrimination method CE-TOF/MS Microbiology QR1-502
Acceso en línea:	https://doaj.org/article/8c6f30865c6b4b7db2baa5b4c4e9fdfc
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spelling	oai:doaj.org-article:8c6f30865c6b4b7db2baa5b4c4e9fdfc2021-11-25T18:20:57ZAnalyzing the Metabolomic Profile of Yellowtail (<i>Seriola quinquerdiata</i>) by Capillary Electrophoresis–Time of Flight Mass Spectrometry to Determine Geographical Origin10.3390/metabo111107932218-1989https://doaj.org/article/8c6f30865c6b4b7db2baa5b4c4e9fdfc2021-11-01T00:00:00Zhttps://www.mdpi.com/2218-1989/11/11/793https://doaj.org/toc/2218-1989Country-of-origin violations have occurred in which some merchants have fraudulently sold cheap Japanese yellowtail (<i>Seriola quinqueradiata</i>) by presenting them as domestic Korean products. There are many methods for determining the origins of marine organisms, such as molecular genetic methods and isotope analysis. However, this study aimed to develop a method for determining the origins of aquatic products using metabolite analysis technology. Ten yellowtail each from Korea and Japan were analyzed by capillary electrophoresis–time of flight/mass spectrometry (CETOF/MS). Hierarchical cluster analysis (HCA) and principal component analysis (PCA) results showed highly differing aspects between the Korean and Japanese samples. In the tricarboxylic acid (TCA) cycle, citric, malic, oxaloglutaric, and fumaric acids exhibited significant differences between Korean and Japanese yellowtail. Sixteen of the twenty essential amino acids analyzed as metabolites also differed significantly. All amino acids were involved in protein digestion, absorption, and metabolism. All 16 amino acid contents were higher in Japanese yellowtail than in Korean yellowtail, except for glutamine. The fasting period was found to be the biggest factor contributing to the difference in amino acid contents, in addition to environmental factors (including feeding habits). These significant differences indicated that metabolomics could be used to determine geographical origin.Jiyoung ShinJunho YangEunji ChaHyunsuk KimYoonhyeung LeeSoi KimIseul ChoiJiyoung YangMDPI AGarticleyellowtailmetabolomicsgeographical origindiscrimination methodCE-TOF/MSMicrobiologyQR1-502ENMetabolites, Vol 11, Iss 793, p 793 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	yellowtail metabolomics geographical origin discrimination method CE-TOF/MS Microbiology QR1-502
spellingShingle	yellowtail metabolomics geographical origin discrimination method CE-TOF/MS Microbiology QR1-502 Jiyoung Shin Junho Yang Eunji Cha Hyunsuk Kim Yoonhyeung Lee Soi Kim Iseul Choi Jiyoung Yang Analyzing the Metabolomic Profile of Yellowtail (<i>Seriola quinquerdiata</i>) by Capillary Electrophoresis–Time of Flight Mass Spectrometry to Determine Geographical Origin
description	Country-of-origin violations have occurred in which some merchants have fraudulently sold cheap Japanese yellowtail (<i>Seriola quinqueradiata</i>) by presenting them as domestic Korean products. There are many methods for determining the origins of marine organisms, such as molecular genetic methods and isotope analysis. However, this study aimed to develop a method for determining the origins of aquatic products using metabolite analysis technology. Ten yellowtail each from Korea and Japan were analyzed by capillary electrophoresis–time of flight/mass spectrometry (CETOF/MS). Hierarchical cluster analysis (HCA) and principal component analysis (PCA) results showed highly differing aspects between the Korean and Japanese samples. In the tricarboxylic acid (TCA) cycle, citric, malic, oxaloglutaric, and fumaric acids exhibited significant differences between Korean and Japanese yellowtail. Sixteen of the twenty essential amino acids analyzed as metabolites also differed significantly. All amino acids were involved in protein digestion, absorption, and metabolism. All 16 amino acid contents were higher in Japanese yellowtail than in Korean yellowtail, except for glutamine. The fasting period was found to be the biggest factor contributing to the difference in amino acid contents, in addition to environmental factors (including feeding habits). These significant differences indicated that metabolomics could be used to determine geographical origin.
format	article
author	Jiyoung Shin Junho Yang Eunji Cha Hyunsuk Kim Yoonhyeung Lee Soi Kim Iseul Choi Jiyoung Yang
author_facet	Jiyoung Shin Junho Yang Eunji Cha Hyunsuk Kim Yoonhyeung Lee Soi Kim Iseul Choi Jiyoung Yang
author_sort	Jiyoung Shin
title	Analyzing the Metabolomic Profile of Yellowtail (<i>Seriola quinquerdiata</i>) by Capillary Electrophoresis–Time of Flight Mass Spectrometry to Determine Geographical Origin
title_short	Analyzing the Metabolomic Profile of Yellowtail (<i>Seriola quinquerdiata</i>) by Capillary Electrophoresis–Time of Flight Mass Spectrometry to Determine Geographical Origin
title_full	Analyzing the Metabolomic Profile of Yellowtail (<i>Seriola quinquerdiata</i>) by Capillary Electrophoresis–Time of Flight Mass Spectrometry to Determine Geographical Origin
title_fullStr	Analyzing the Metabolomic Profile of Yellowtail (<i>Seriola quinquerdiata</i>) by Capillary Electrophoresis–Time of Flight Mass Spectrometry to Determine Geographical Origin
title_full_unstemmed	Analyzing the Metabolomic Profile of Yellowtail (<i>Seriola quinquerdiata</i>) by Capillary Electrophoresis–Time of Flight Mass Spectrometry to Determine Geographical Origin
title_sort	analyzing the metabolomic profile of yellowtail (<i>seriola quinquerdiata</i>) by capillary electrophoresis–time of flight mass spectrometry to determine geographical origin
publisher	MDPI AG
publishDate	2021
url	https://doaj.org/article/8c6f30865c6b4b7db2baa5b4c4e9fdfc
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Analyzing the Metabolomic Profile of Yellowtail (<i>Seriola quinquerdiata</i>) by Capillary Electrophoresis–Time of Flight Mass Spectrometry to Determine Geographical Origin

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