Distribution of primed T cells and antigen-loaded antigen presenting cells following intranasal immunization in mice.

Priming of T cells is a key event in vaccination, since it bears a decisive influence on the type and magnitude of the immune response. T-cell priming after mucosal immunization via the nasal route was studied by investigating the distribution of antigen-loaded antigen presenting cells (APCs) and pr...

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Autores principales:	Annalisa Ciabattini, Elena Pettini, Fabio Fiorino, Gennaro Prota, Gianni Pozzi, Donata Medaglini
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2011
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/8c769d358800441283c825ff59a703e5
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spelling	oai:doaj.org-article:8c769d358800441283c825ff59a703e52021-11-18T06:54:49ZDistribution of primed T cells and antigen-loaded antigen presenting cells following intranasal immunization in mice.1932-620310.1371/journal.pone.0019346https://doaj.org/article/8c769d358800441283c825ff59a703e52011-04-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/21559409/?tool=EBIhttps://doaj.org/toc/1932-6203Priming of T cells is a key event in vaccination, since it bears a decisive influence on the type and magnitude of the immune response. T-cell priming after mucosal immunization via the nasal route was studied by investigating the distribution of antigen-loaded antigen presenting cells (APCs) and primed antigen-specific T cells. Nasal immunization studies were conducted using the model protein antigen ovalbumin (OVA) plus CpG oligodeoxynucleotide adjuvant. Trafficking of antigen-specific primed T cells was analyzed in vivo after adoptive transfer of OVA-specific transgenic T cells in the presence or absence of fingolimod, a drug that causes lymphocytes sequestration within lymph nodes. Antigen-loaded APCs were observed in mediastinal lymph nodes, draining the respiratory tract, but not in distal lymph nodes. Antigen-specific proliferating T cells were first observed within draining lymph nodes, and later in distal iliac and mesenteric lymph nodes and in the spleen. The presence at distal sites was due to migration of locally primed T cells as shown by fingolimod treatment that caused a drastic reduction of proliferated T cells in non-draining lymph nodes and an accumulation of extensively divided T cells within draining lymph nodes. Homing of nasally primed T cells in distal iliac lymph nodes was CD62L-dependent, while entry into mesenteric lymph nodes depended on both CD62L and α4β7, as shown by in vivo antibody-mediated inhibition of T-cell trafficking. These data, elucidating the trafficking of antigen-specific primed T cells to non-draining peripheral and mucosa-associated lymph nodes following nasal immunization, provide relevant insights for the design of vaccination strategies based on mucosal priming.Annalisa CiabattiniElena PettiniFabio FiorinoGennaro ProtaGianni PozziDonata MedagliniPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 6, Iss 4, p e19346 (2011)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Annalisa Ciabattini Elena Pettini Fabio Fiorino Gennaro Prota Gianni Pozzi Donata Medaglini Distribution of primed T cells and antigen-loaded antigen presenting cells following intranasal immunization in mice.
description	Priming of T cells is a key event in vaccination, since it bears a decisive influence on the type and magnitude of the immune response. T-cell priming after mucosal immunization via the nasal route was studied by investigating the distribution of antigen-loaded antigen presenting cells (APCs) and primed antigen-specific T cells. Nasal immunization studies were conducted using the model protein antigen ovalbumin (OVA) plus CpG oligodeoxynucleotide adjuvant. Trafficking of antigen-specific primed T cells was analyzed in vivo after adoptive transfer of OVA-specific transgenic T cells in the presence or absence of fingolimod, a drug that causes lymphocytes sequestration within lymph nodes. Antigen-loaded APCs were observed in mediastinal lymph nodes, draining the respiratory tract, but not in distal lymph nodes. Antigen-specific proliferating T cells were first observed within draining lymph nodes, and later in distal iliac and mesenteric lymph nodes and in the spleen. The presence at distal sites was due to migration of locally primed T cells as shown by fingolimod treatment that caused a drastic reduction of proliferated T cells in non-draining lymph nodes and an accumulation of extensively divided T cells within draining lymph nodes. Homing of nasally primed T cells in distal iliac lymph nodes was CD62L-dependent, while entry into mesenteric lymph nodes depended on both CD62L and α4β7, as shown by in vivo antibody-mediated inhibition of T-cell trafficking. These data, elucidating the trafficking of antigen-specific primed T cells to non-draining peripheral and mucosa-associated lymph nodes following nasal immunization, provide relevant insights for the design of vaccination strategies based on mucosal priming.
format	article
author	Annalisa Ciabattini Elena Pettini Fabio Fiorino Gennaro Prota Gianni Pozzi Donata Medaglini
author_facet	Annalisa Ciabattini Elena Pettini Fabio Fiorino Gennaro Prota Gianni Pozzi Donata Medaglini
author_sort	Annalisa Ciabattini
title	Distribution of primed T cells and antigen-loaded antigen presenting cells following intranasal immunization in mice.
title_short	Distribution of primed T cells and antigen-loaded antigen presenting cells following intranasal immunization in mice.
title_full	Distribution of primed T cells and antigen-loaded antigen presenting cells following intranasal immunization in mice.
title_fullStr	Distribution of primed T cells and antigen-loaded antigen presenting cells following intranasal immunization in mice.
title_full_unstemmed	Distribution of primed T cells and antigen-loaded antigen presenting cells following intranasal immunization in mice.
title_sort	distribution of primed t cells and antigen-loaded antigen presenting cells following intranasal immunization in mice.
publisher	Public Library of Science (PLoS)
publishDate	2011
url	https://doaj.org/article/8c769d358800441283c825ff59a703e5
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Distribution of primed T cells and antigen-loaded antigen presenting cells following intranasal immunization in mice.

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