Spatial-resolved metabolomics reveals tissue-specific metabolic reprogramming in diabetic nephropathy by using mass spectrometry imaging

Detailed knowledge on tissue-specific metabolic reprogramming in diabetic nephropathy (DN) is vital for more accurate understanding the molecular pathological signature and developing novel therapeutic strategies. In the present study, a spatial-resolved metabolomics approach based on air flow-assis...

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Autores principales:	Zhonghua Wang, Wenqing Fu, Meiling Huo, Bingshu He, Yaqi Liu, Lu Tian, Wanfang Li, Zhi Zhou, Baili Wang, Jianzhen Xia, Yanhua Chen, Jinfeng Wei, Zeper Abliz
Formato:	article
Lenguaje:	EN
Publicado:	Elsevier 2021
Materias:	Spatial-resolved metabolomics Mass spectrometry imaging Diabetic nephropathy Metabolic reprogramming Astragaloside IV Therapeutics. Pharmacology RM1-950
Acceso en línea:	https://doaj.org/article/8c8093def1ee49059a4b1d9e28a4d4c7
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spelling	oai:doaj.org-article:8c8093def1ee49059a4b1d9e28a4d4c72021-12-02T05:01:24ZSpatial-resolved metabolomics reveals tissue-specific metabolic reprogramming in diabetic nephropathy by using mass spectrometry imaging2211-383510.1016/j.apsb.2021.05.013https://doaj.org/article/8c8093def1ee49059a4b1d9e28a4d4c72021-11-01T00:00:00Zhttp://www.sciencedirect.com/science/article/pii/S2211383521001799https://doaj.org/toc/2211-3835Detailed knowledge on tissue-specific metabolic reprogramming in diabetic nephropathy (DN) is vital for more accurate understanding the molecular pathological signature and developing novel therapeutic strategies. In the present study, a spatial-resolved metabolomics approach based on air flow-assisted desorption electrospray ionization (AFADESI) and matrix-assisted laser desorption ionization (MALDI) integrated mass spectrometry imaging (MSI) was proposed to investigate tissue-specific metabolic alterations in the kidneys of high-fat diet-fed and streptozotocin (STZ)-treated DN rats and the therapeutic effect of astragaloside IV, a potential anti-diabetic drug, against DN. As a result, a wide range of functional metabolites including sugars, amino acids, nucleotides and their derivatives, fatty acids, phospholipids, sphingolipids, glycerides, carnitine and its derivatives, vitamins, peptides, and metal ions associated with DN were identified and their unique distribution patterns in the rat kidney were visualized with high chemical specificity and high spatial resolution. These region-specific metabolic disturbances were ameliorated by repeated oral administration of astragaloside IV (100 mg/kg) for 12 weeks. This study provided more comprehensive and detailed information about the tissue-specific metabolic reprogramming and molecular pathological signature in the kidney of diabetic rats. These findings highlighted the promising potential of AFADESI and MALDI integrated MSI based metabolomics approach for application in metabolic kidney diseases.Zhonghua WangWenqing FuMeiling HuoBingshu HeYaqi LiuLu TianWanfang LiZhi ZhouBaili WangJianzhen XiaYanhua ChenJinfeng WeiZeper AblizElsevierarticleSpatial-resolved metabolomicsMass spectrometry imagingDiabetic nephropathyMetabolic reprogrammingAstragaloside IVTherapeutics. PharmacologyRM1-950ENActa Pharmaceutica Sinica B, Vol 11, Iss 11, Pp 3665-3677 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Spatial-resolved metabolomics Mass spectrometry imaging Diabetic nephropathy Metabolic reprogramming Astragaloside IV Therapeutics. Pharmacology RM1-950
spellingShingle	Spatial-resolved metabolomics Mass spectrometry imaging Diabetic nephropathy Metabolic reprogramming Astragaloside IV Therapeutics. Pharmacology RM1-950 Zhonghua Wang Wenqing Fu Meiling Huo Bingshu He Yaqi Liu Lu Tian Wanfang Li Zhi Zhou Baili Wang Jianzhen Xia Yanhua Chen Jinfeng Wei Zeper Abliz Spatial-resolved metabolomics reveals tissue-specific metabolic reprogramming in diabetic nephropathy by using mass spectrometry imaging
description	Detailed knowledge on tissue-specific metabolic reprogramming in diabetic nephropathy (DN) is vital for more accurate understanding the molecular pathological signature and developing novel therapeutic strategies. In the present study, a spatial-resolved metabolomics approach based on air flow-assisted desorption electrospray ionization (AFADESI) and matrix-assisted laser desorption ionization (MALDI) integrated mass spectrometry imaging (MSI) was proposed to investigate tissue-specific metabolic alterations in the kidneys of high-fat diet-fed and streptozotocin (STZ)-treated DN rats and the therapeutic effect of astragaloside IV, a potential anti-diabetic drug, against DN. As a result, a wide range of functional metabolites including sugars, amino acids, nucleotides and their derivatives, fatty acids, phospholipids, sphingolipids, glycerides, carnitine and its derivatives, vitamins, peptides, and metal ions associated with DN were identified and their unique distribution patterns in the rat kidney were visualized with high chemical specificity and high spatial resolution. These region-specific metabolic disturbances were ameliorated by repeated oral administration of astragaloside IV (100 mg/kg) for 12 weeks. This study provided more comprehensive and detailed information about the tissue-specific metabolic reprogramming and molecular pathological signature in the kidney of diabetic rats. These findings highlighted the promising potential of AFADESI and MALDI integrated MSI based metabolomics approach for application in metabolic kidney diseases.
format	article
author	Zhonghua Wang Wenqing Fu Meiling Huo Bingshu He Yaqi Liu Lu Tian Wanfang Li Zhi Zhou Baili Wang Jianzhen Xia Yanhua Chen Jinfeng Wei Zeper Abliz
author_facet	Zhonghua Wang Wenqing Fu Meiling Huo Bingshu He Yaqi Liu Lu Tian Wanfang Li Zhi Zhou Baili Wang Jianzhen Xia Yanhua Chen Jinfeng Wei Zeper Abliz
author_sort	Zhonghua Wang
title	Spatial-resolved metabolomics reveals tissue-specific metabolic reprogramming in diabetic nephropathy by using mass spectrometry imaging
title_short	Spatial-resolved metabolomics reveals tissue-specific metabolic reprogramming in diabetic nephropathy by using mass spectrometry imaging
title_full	Spatial-resolved metabolomics reveals tissue-specific metabolic reprogramming in diabetic nephropathy by using mass spectrometry imaging
title_fullStr	Spatial-resolved metabolomics reveals tissue-specific metabolic reprogramming in diabetic nephropathy by using mass spectrometry imaging
title_full_unstemmed	Spatial-resolved metabolomics reveals tissue-specific metabolic reprogramming in diabetic nephropathy by using mass spectrometry imaging
title_sort	spatial-resolved metabolomics reveals tissue-specific metabolic reprogramming in diabetic nephropathy by using mass spectrometry imaging
publisher	Elsevier
publishDate	2021
url	https://doaj.org/article/8c8093def1ee49059a4b1d9e28a4d4c7
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Spatial-resolved metabolomics reveals tissue-specific metabolic reprogramming in diabetic nephropathy by using mass spectrometry imaging

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