The Integrity of the HMR complex is necessary for centromeric binding and reproductive isolation in Drosophila.

Postzygotic isolation by genomic conflict is a major cause for the formation of species. Despite its importance, the molecular mechanisms that result in the lethality of interspecies hybrids are still largely unclear. The genus Drosophila, which contains over 1600 different species, is one of the be...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Andrea Lukacs, Andreas W Thomae, Peter Krueger, Tamas Schauer, Anuroop V Venkatasubramani, Natalia Y Kochanova, Wasim Aftab, Rupam Choudhury, Ignasi Forne, Axel Imhof
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2021
Materias:	Genetics QH426-470
Acceso en línea:	https://doaj.org/article/8c8556f9eaf742c38d19bf03b185c7dd
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:8c8556f9eaf742c38d19bf03b185c7dd
record_format	dspace
spelling	oai:doaj.org-article:8c8556f9eaf742c38d19bf03b185c7dd2021-12-02T20:02:51ZThe Integrity of the HMR complex is necessary for centromeric binding and reproductive isolation in Drosophila.1553-73901553-740410.1371/journal.pgen.1009744https://doaj.org/article/8c8556f9eaf742c38d19bf03b185c7dd2021-08-01T00:00:00Zhttps://doi.org/10.1371/journal.pgen.1009744https://doaj.org/toc/1553-7390https://doaj.org/toc/1553-7404Postzygotic isolation by genomic conflict is a major cause for the formation of species. Despite its importance, the molecular mechanisms that result in the lethality of interspecies hybrids are still largely unclear. The genus Drosophila, which contains over 1600 different species, is one of the best characterized model systems to study these questions. We showed in the past that the expression levels of the two hybrid incompatibility factors Hmr and Lhr diverged in the two closely related Drosophila species, D. melanogaster and D. simulans, resulting in an increased level of both proteins in interspecies hybrids. The overexpression of the two proteins also leads to mitotic defects, a misregulation in the expression of transposable elements and decreased fertility in pure species. In this work, we describe a distinct six subunit protein complex containing HMR and LHR and analyse the effect of Hmr mutations on complex integrity and function. Our experiments suggest that HMR needs to bring together components of centromeric and pericentromeric chromatin to fulfil its physiological function and to cause hybrid male lethality.Andrea LukacsAndreas W ThomaePeter KruegerTamas SchauerAnuroop V VenkatasubramaniNatalia Y KochanovaWasim AftabRupam ChoudhuryIgnasi ForneAxel ImhofPublic Library of Science (PLoS)articleGeneticsQH426-470ENPLoS Genetics, Vol 17, Iss 8, p e1009744 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Genetics QH426-470
spellingShingle	Genetics QH426-470 Andrea Lukacs Andreas W Thomae Peter Krueger Tamas Schauer Anuroop V Venkatasubramani Natalia Y Kochanova Wasim Aftab Rupam Choudhury Ignasi Forne Axel Imhof The Integrity of the HMR complex is necessary for centromeric binding and reproductive isolation in Drosophila.
description	Postzygotic isolation by genomic conflict is a major cause for the formation of species. Despite its importance, the molecular mechanisms that result in the lethality of interspecies hybrids are still largely unclear. The genus Drosophila, which contains over 1600 different species, is one of the best characterized model systems to study these questions. We showed in the past that the expression levels of the two hybrid incompatibility factors Hmr and Lhr diverged in the two closely related Drosophila species, D. melanogaster and D. simulans, resulting in an increased level of both proteins in interspecies hybrids. The overexpression of the two proteins also leads to mitotic defects, a misregulation in the expression of transposable elements and decreased fertility in pure species. In this work, we describe a distinct six subunit protein complex containing HMR and LHR and analyse the effect of Hmr mutations on complex integrity and function. Our experiments suggest that HMR needs to bring together components of centromeric and pericentromeric chromatin to fulfil its physiological function and to cause hybrid male lethality.
format	article
author	Andrea Lukacs Andreas W Thomae Peter Krueger Tamas Schauer Anuroop V Venkatasubramani Natalia Y Kochanova Wasim Aftab Rupam Choudhury Ignasi Forne Axel Imhof
author_facet	Andrea Lukacs Andreas W Thomae Peter Krueger Tamas Schauer Anuroop V Venkatasubramani Natalia Y Kochanova Wasim Aftab Rupam Choudhury Ignasi Forne Axel Imhof
author_sort	Andrea Lukacs
title	The Integrity of the HMR complex is necessary for centromeric binding and reproductive isolation in Drosophila.
title_short	The Integrity of the HMR complex is necessary for centromeric binding and reproductive isolation in Drosophila.
title_full	The Integrity of the HMR complex is necessary for centromeric binding and reproductive isolation in Drosophila.
title_fullStr	The Integrity of the HMR complex is necessary for centromeric binding and reproductive isolation in Drosophila.
title_full_unstemmed	The Integrity of the HMR complex is necessary for centromeric binding and reproductive isolation in Drosophila.
title_sort	integrity of the hmr complex is necessary for centromeric binding and reproductive isolation in drosophila.
publisher	Public Library of Science (PLoS)
publishDate	2021
url	https://doaj.org/article/8c8556f9eaf742c38d19bf03b185c7dd
work_keys_str_mv	AT andrealukacs theintegrityofthehmrcomplexisnecessaryforcentromericbindingandreproductiveisolationindrosophila AT andreaswthomae theintegrityofthehmrcomplexisnecessaryforcentromericbindingandreproductiveisolationindrosophila AT peterkrueger theintegrityofthehmrcomplexisnecessaryforcentromericbindingandreproductiveisolationindrosophila AT tamasschauer theintegrityofthehmrcomplexisnecessaryforcentromericbindingandreproductiveisolationindrosophila AT anuroopvvenkatasubramani theintegrityofthehmrcomplexisnecessaryforcentromericbindingandreproductiveisolationindrosophila AT nataliaykochanova theintegrityofthehmrcomplexisnecessaryforcentromericbindingandreproductiveisolationindrosophila AT wasimaftab theintegrityofthehmrcomplexisnecessaryforcentromericbindingandreproductiveisolationindrosophila AT rupamchoudhury theintegrityofthehmrcomplexisnecessaryforcentromericbindingandreproductiveisolationindrosophila AT ignasiforne theintegrityofthehmrcomplexisnecessaryforcentromericbindingandreproductiveisolationindrosophila AT axelimhof theintegrityofthehmrcomplexisnecessaryforcentromericbindingandreproductiveisolationindrosophila AT andrealukacs integrityofthehmrcomplexisnecessaryforcentromericbindingandreproductiveisolationindrosophila AT andreaswthomae integrityofthehmrcomplexisnecessaryforcentromericbindingandreproductiveisolationindrosophila AT peterkrueger integrityofthehmrcomplexisnecessaryforcentromericbindingandreproductiveisolationindrosophila AT tamasschauer integrityofthehmrcomplexisnecessaryforcentromericbindingandreproductiveisolationindrosophila AT anuroopvvenkatasubramani integrityofthehmrcomplexisnecessaryforcentromericbindingandreproductiveisolationindrosophila AT nataliaykochanova integrityofthehmrcomplexisnecessaryforcentromericbindingandreproductiveisolationindrosophila AT wasimaftab integrityofthehmrcomplexisnecessaryforcentromericbindingandreproductiveisolationindrosophila AT rupamchoudhury integrityofthehmrcomplexisnecessaryforcentromericbindingandreproductiveisolationindrosophila AT ignasiforne integrityofthehmrcomplexisnecessaryforcentromericbindingandreproductiveisolationindrosophila AT axelimhof integrityofthehmrcomplexisnecessaryforcentromericbindingandreproductiveisolationindrosophila
_version_	1718375671431954432

The Integrity of the HMR complex is necessary for centromeric binding and reproductive isolation in Drosophila.

Ejemplares similares