An in-depth analysis of the mitochondrial phylogenetic landscape of Cambodia

Abstract Cambodia harbours a variety of human aboriginal populations that have scarcely been studied in terms of genetic diversity of entire mitochondrial genomes. Here we present the matrilineal gene pool of 299 Cambodian refugees from three different ethnic groups (Cham, Khmer, and Khmer Loeu) der...

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Autores principales:	Anita Kloss-Brandstätter, Monika Summerer, David Horst, Basil Horst, Gertraud Streiter, Julia Raschenberger, Florian Kronenberg, Torpong Sanguansermsri, Jürgen Horst, Hansi Weissensteiner
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/8c959a9f0f054139a72eaecab9bc4e55
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spelling	oai:doaj.org-article:8c959a9f0f054139a72eaecab9bc4e552021-12-02T14:42:01ZAn in-depth analysis of the mitochondrial phylogenetic landscape of Cambodia10.1038/s41598-021-90145-22045-2322https://doaj.org/article/8c959a9f0f054139a72eaecab9bc4e552021-05-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-90145-2https://doaj.org/toc/2045-2322Abstract Cambodia harbours a variety of human aboriginal populations that have scarcely been studied in terms of genetic diversity of entire mitochondrial genomes. Here we present the matrilineal gene pool of 299 Cambodian refugees from three different ethnic groups (Cham, Khmer, and Khmer Loeu) deriving from 16 Cambodian districts. After establishing a DNA-saving high-throughput strategy for mitochondrial whole-genome Sanger sequencing, a HaploGrep based workflow was used for quality control, haplogroup classification and phylogenetic reconstruction. The application of diverse phylogenetic algorithms revealed an exciting picture of the genetic diversity of Cambodia, especially in relation to populations from Southeast Asia and from the whole world. A total of 224 unique haplotypes were identified, which were mostly classified under haplogroups B5a1, F1a1, or categorized as newly defined basal haplogroups or basal sub-branches of R, N and M clades. The presence of autochthonous maternal lineages could be confirmed as reported in previous studies. The exceptional homogeneity observed between and within the three investigated Cambodian ethnic groups indicates genetic isolation of the whole population. Between ethnicities, genetic barriers were not detected. The mtDNA data presented here increases the phylogenetic resolution in Cambodia significantly, thereby highlighting the need for an update of the current human mtDNA phylogeny.Anita Kloss-BrandstätterMonika SummererDavid HorstBasil HorstGertraud StreiterJulia RaschenbergerFlorian KronenbergTorpong SanguansermsriJürgen HorstHansi WeissensteinerNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-10 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Anita Kloss-Brandstätter Monika Summerer David Horst Basil Horst Gertraud Streiter Julia Raschenberger Florian Kronenberg Torpong Sanguansermsri Jürgen Horst Hansi Weissensteiner An in-depth analysis of the mitochondrial phylogenetic landscape of Cambodia
description	Abstract Cambodia harbours a variety of human aboriginal populations that have scarcely been studied in terms of genetic diversity of entire mitochondrial genomes. Here we present the matrilineal gene pool of 299 Cambodian refugees from three different ethnic groups (Cham, Khmer, and Khmer Loeu) deriving from 16 Cambodian districts. After establishing a DNA-saving high-throughput strategy for mitochondrial whole-genome Sanger sequencing, a HaploGrep based workflow was used for quality control, haplogroup classification and phylogenetic reconstruction. The application of diverse phylogenetic algorithms revealed an exciting picture of the genetic diversity of Cambodia, especially in relation to populations from Southeast Asia and from the whole world. A total of 224 unique haplotypes were identified, which were mostly classified under haplogroups B5a1, F1a1, or categorized as newly defined basal haplogroups or basal sub-branches of R, N and M clades. The presence of autochthonous maternal lineages could be confirmed as reported in previous studies. The exceptional homogeneity observed between and within the three investigated Cambodian ethnic groups indicates genetic isolation of the whole population. Between ethnicities, genetic barriers were not detected. The mtDNA data presented here increases the phylogenetic resolution in Cambodia significantly, thereby highlighting the need for an update of the current human mtDNA phylogeny.
format	article
author	Anita Kloss-Brandstätter Monika Summerer David Horst Basil Horst Gertraud Streiter Julia Raschenberger Florian Kronenberg Torpong Sanguansermsri Jürgen Horst Hansi Weissensteiner
author_facet	Anita Kloss-Brandstätter Monika Summerer David Horst Basil Horst Gertraud Streiter Julia Raschenberger Florian Kronenberg Torpong Sanguansermsri Jürgen Horst Hansi Weissensteiner
author_sort	Anita Kloss-Brandstätter
title	An in-depth analysis of the mitochondrial phylogenetic landscape of Cambodia
title_short	An in-depth analysis of the mitochondrial phylogenetic landscape of Cambodia
title_full	An in-depth analysis of the mitochondrial phylogenetic landscape of Cambodia
title_fullStr	An in-depth analysis of the mitochondrial phylogenetic landscape of Cambodia
title_full_unstemmed	An in-depth analysis of the mitochondrial phylogenetic landscape of Cambodia
title_sort	in-depth analysis of the mitochondrial phylogenetic landscape of cambodia
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/8c959a9f0f054139a72eaecab9bc4e55
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An in-depth analysis of the mitochondrial phylogenetic landscape of Cambodia

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