A nanobody-based fluorescent reporter reveals human α-synuclein in the cell cytosol

α-Syn in CSF is a biomarker of neurodegenerative diseases; however, the detection of clinically relevant species is difficult. Here, the authors create a nanobody biosensor that reveals the presence of α-Syn in cells, which allow the detection of transmittable forms of α-Syn present in human CSF.

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Autores principales:	Christoph Gerdes, Natalia Waal, Thomas Offner, Eugenio F. Fornasiero, Nora Wender, Hannes Verbarg, Ivan Manzini, Claudia Trenkwalder, Brit Mollenhauer, Timo Strohäker, Markus Zweckstetter, Stefan Becker, Silvio O. Rizzoli, Fitnat Buket Basmanav, Felipe Opazo
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2020
Materias:	Science Q
Acceso en línea:	https://doaj.org/article/8c988c42912b4775afde11da5ff67e31
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spelling	oai:doaj.org-article:8c988c42912b4775afde11da5ff67e312021-12-02T18:24:59ZA nanobody-based fluorescent reporter reveals human α-synuclein in the cell cytosol10.1038/s41467-020-16575-02041-1723https://doaj.org/article/8c988c42912b4775afde11da5ff67e312020-06-01T00:00:00Zhttps://doi.org/10.1038/s41467-020-16575-0https://doaj.org/toc/2041-1723α-Syn in CSF is a biomarker of neurodegenerative diseases; however, the detection of clinically relevant species is difficult. Here, the authors create a nanobody biosensor that reveals the presence of α-Syn in cells, which allow the detection of transmittable forms of α-Syn present in human CSF.Christoph GerdesNatalia WaalThomas OffnerEugenio F. FornasieroNora WenderHannes VerbargIvan ManziniClaudia TrenkwalderBrit MollenhauerTimo StrohäkerMarkus ZweckstetterStefan BeckerSilvio O. RizzoliFitnat Buket BasmanavFelipe OpazoNature PortfolioarticleScienceQENNature Communications, Vol 11, Iss 1, Pp 1-13 (2020)
institution	DOAJ
collection	DOAJ
language	EN
topic	Science Q
spellingShingle	Science Q Christoph Gerdes Natalia Waal Thomas Offner Eugenio F. Fornasiero Nora Wender Hannes Verbarg Ivan Manzini Claudia Trenkwalder Brit Mollenhauer Timo Strohäker Markus Zweckstetter Stefan Becker Silvio O. Rizzoli Fitnat Buket Basmanav Felipe Opazo A nanobody-based fluorescent reporter reveals human α-synuclein in the cell cytosol
description	α-Syn in CSF is a biomarker of neurodegenerative diseases; however, the detection of clinically relevant species is difficult. Here, the authors create a nanobody biosensor that reveals the presence of α-Syn in cells, which allow the detection of transmittable forms of α-Syn present in human CSF.
format	article
author	Christoph Gerdes Natalia Waal Thomas Offner Eugenio F. Fornasiero Nora Wender Hannes Verbarg Ivan Manzini Claudia Trenkwalder Brit Mollenhauer Timo Strohäker Markus Zweckstetter Stefan Becker Silvio O. Rizzoli Fitnat Buket Basmanav Felipe Opazo
author_facet	Christoph Gerdes Natalia Waal Thomas Offner Eugenio F. Fornasiero Nora Wender Hannes Verbarg Ivan Manzini Claudia Trenkwalder Brit Mollenhauer Timo Strohäker Markus Zweckstetter Stefan Becker Silvio O. Rizzoli Fitnat Buket Basmanav Felipe Opazo
author_sort	Christoph Gerdes
title	A nanobody-based fluorescent reporter reveals human α-synuclein in the cell cytosol
title_short	A nanobody-based fluorescent reporter reveals human α-synuclein in the cell cytosol
title_full	A nanobody-based fluorescent reporter reveals human α-synuclein in the cell cytosol
title_fullStr	A nanobody-based fluorescent reporter reveals human α-synuclein in the cell cytosol
title_full_unstemmed	A nanobody-based fluorescent reporter reveals human α-synuclein in the cell cytosol
title_sort	nanobody-based fluorescent reporter reveals human α-synuclein in the cell cytosol
publisher	Nature Portfolio
publishDate	2020
url	https://doaj.org/article/8c988c42912b4775afde11da5ff67e31
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A nanobody-based fluorescent reporter reveals human α-synuclein in the cell cytosol

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