The role of the multiple banded antigen of Ureaplasma parvum in intra-amniotic infection: major virulence factor or decoy?
The multiple banded antigen (MBA) is a predicted virulence factor of Ureaplasma species. Antigenic variation of the MBA is a potential mechanism by which ureaplasmas avoid immune recognition and cause chronic infections of the upper genital tract of pregnant women. We tested whether the MBA is invol...
Guardado en:
| Autores principales: | , , , , , , , , |
|---|---|
| Formato: | article |
| Lenguaje: | EN |
| Publicado: |
Public Library of Science (PLoS)
2012
|
| Materias: | |
| Acceso en línea: | https://doaj.org/article/8c98fd35bb584d329f0fcb4a3ec0b0e2 |
| Etiquetas: |
Agregar Etiqueta
Sin Etiquetas, Sea el primero en etiquetar este registro!
|
| id |
oai:doaj.org-article:8c98fd35bb584d329f0fcb4a3ec0b0e2 |
|---|---|
| record_format |
dspace |
| spelling |
oai:doaj.org-article:8c98fd35bb584d329f0fcb4a3ec0b0e22021-11-18T07:30:22ZThe role of the multiple banded antigen of Ureaplasma parvum in intra-amniotic infection: major virulence factor or decoy?1932-620310.1371/journal.pone.0029856https://doaj.org/article/8c98fd35bb584d329f0fcb4a3ec0b0e22012-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/22253806/?tool=EBIhttps://doaj.org/toc/1932-6203The multiple banded antigen (MBA) is a predicted virulence factor of Ureaplasma species. Antigenic variation of the MBA is a potential mechanism by which ureaplasmas avoid immune recognition and cause chronic infections of the upper genital tract of pregnant women. We tested whether the MBA is involved in the pathogenesis of intra-amniotic infection and chorioamnionitis by injecting virulent or avirulent-derived ureaplasma clones (expressing single MBA variants) into the amniotic fluid of pregnant sheep. At 55 days of gestation pregnant ewes (n = 20) received intra-amniotic injections of virulent-derived or avirulent-derived U. parvum serovar 6 strains (2×10⁴ CFU), or 10B medium (n = 5). Amniotic fluid was collected every two weeks post-infection and fetal tissues were collected at the time of surgical delivery of the fetus (140 days of gestation). Whilst chronic colonisation was established in the amniotic fluid of animals infected with avirulent-derived and virulent-derived ureaplasmas, the severity of chorioamnionitis and fetal inflammation was not different between these groups (p>0.05). MBA size variants (32-170 kDa) were generated in vivo in amniotic fluid samples from both the avirulent and virulent groups, whereas in vitro antibody selection experiments led to the emergence of MBA-negative escape variants in both strains. Anti-ureaplasma IgG antibodies were detected in the maternal serum of animals from the avirulent (40%) and virulent (55%) groups, and these antibodies correlated with increased IL-1β, IL-6 and IL-8 expression in chorioamnion tissue (p<0.05). We demonstrate that ureaplasmas are capable of MBA phase variation in vitro; however, ureaplasmas undergo MBA size variation in vivo, to potentially prevent eradication by the immune response. Size variation of the MBA did not correlate with the severity of chorioamnionitis. Nonetheless, the correlation between a maternal humoral response and the expression of chorioamnion cytokines is a novel finding. This host response may be important in the pathogenesis of inflammation-mediated adverse pregnancy outcomes.Samantha J DandoIlias NitsosSuhas G KallapurJohn P NewnhamGraeme R PolglaseJ Jane PillowAlan H JobePeter TimmsChristine L KnoxPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 7, Iss 1, p e29856 (2012) |
| institution |
DOAJ |
| collection |
DOAJ |
| language |
EN |
| topic |
Medicine R Science Q |
| spellingShingle |
Medicine R Science Q Samantha J Dando Ilias Nitsos Suhas G Kallapur John P Newnham Graeme R Polglase J Jane Pillow Alan H Jobe Peter Timms Christine L Knox The role of the multiple banded antigen of Ureaplasma parvum in intra-amniotic infection: major virulence factor or decoy? |
| description |
The multiple banded antigen (MBA) is a predicted virulence factor of Ureaplasma species. Antigenic variation of the MBA is a potential mechanism by which ureaplasmas avoid immune recognition and cause chronic infections of the upper genital tract of pregnant women. We tested whether the MBA is involved in the pathogenesis of intra-amniotic infection and chorioamnionitis by injecting virulent or avirulent-derived ureaplasma clones (expressing single MBA variants) into the amniotic fluid of pregnant sheep. At 55 days of gestation pregnant ewes (n = 20) received intra-amniotic injections of virulent-derived or avirulent-derived U. parvum serovar 6 strains (2×10⁴ CFU), or 10B medium (n = 5). Amniotic fluid was collected every two weeks post-infection and fetal tissues were collected at the time of surgical delivery of the fetus (140 days of gestation). Whilst chronic colonisation was established in the amniotic fluid of animals infected with avirulent-derived and virulent-derived ureaplasmas, the severity of chorioamnionitis and fetal inflammation was not different between these groups (p>0.05). MBA size variants (32-170 kDa) were generated in vivo in amniotic fluid samples from both the avirulent and virulent groups, whereas in vitro antibody selection experiments led to the emergence of MBA-negative escape variants in both strains. Anti-ureaplasma IgG antibodies were detected in the maternal serum of animals from the avirulent (40%) and virulent (55%) groups, and these antibodies correlated with increased IL-1β, IL-6 and IL-8 expression in chorioamnion tissue (p<0.05). We demonstrate that ureaplasmas are capable of MBA phase variation in vitro; however, ureaplasmas undergo MBA size variation in vivo, to potentially prevent eradication by the immune response. Size variation of the MBA did not correlate with the severity of chorioamnionitis. Nonetheless, the correlation between a maternal humoral response and the expression of chorioamnion cytokines is a novel finding. This host response may be important in the pathogenesis of inflammation-mediated adverse pregnancy outcomes. |
| format |
article |
| author |
Samantha J Dando Ilias Nitsos Suhas G Kallapur John P Newnham Graeme R Polglase J Jane Pillow Alan H Jobe Peter Timms Christine L Knox |
| author_facet |
Samantha J Dando Ilias Nitsos Suhas G Kallapur John P Newnham Graeme R Polglase J Jane Pillow Alan H Jobe Peter Timms Christine L Knox |
| author_sort |
Samantha J Dando |
| title |
The role of the multiple banded antigen of Ureaplasma parvum in intra-amniotic infection: major virulence factor or decoy? |
| title_short |
The role of the multiple banded antigen of Ureaplasma parvum in intra-amniotic infection: major virulence factor or decoy? |
| title_full |
The role of the multiple banded antigen of Ureaplasma parvum in intra-amniotic infection: major virulence factor or decoy? |
| title_fullStr |
The role of the multiple banded antigen of Ureaplasma parvum in intra-amniotic infection: major virulence factor or decoy? |
| title_full_unstemmed |
The role of the multiple banded antigen of Ureaplasma parvum in intra-amniotic infection: major virulence factor or decoy? |
| title_sort |
role of the multiple banded antigen of ureaplasma parvum in intra-amniotic infection: major virulence factor or decoy? |
| publisher |
Public Library of Science (PLoS) |
| publishDate |
2012 |
| url |
https://doaj.org/article/8c98fd35bb584d329f0fcb4a3ec0b0e2 |
| work_keys_str_mv |
AT samanthajdando theroleofthemultiplebandedantigenofureaplasmaparvuminintraamnioticinfectionmajorvirulencefactorordecoy AT iliasnitsos theroleofthemultiplebandedantigenofureaplasmaparvuminintraamnioticinfectionmajorvirulencefactorordecoy AT suhasgkallapur theroleofthemultiplebandedantigenofureaplasmaparvuminintraamnioticinfectionmajorvirulencefactorordecoy AT johnpnewnham theroleofthemultiplebandedantigenofureaplasmaparvuminintraamnioticinfectionmajorvirulencefactorordecoy AT graemerpolglase theroleofthemultiplebandedantigenofureaplasmaparvuminintraamnioticinfectionmajorvirulencefactorordecoy AT jjanepillow theroleofthemultiplebandedantigenofureaplasmaparvuminintraamnioticinfectionmajorvirulencefactorordecoy AT alanhjobe theroleofthemultiplebandedantigenofureaplasmaparvuminintraamnioticinfectionmajorvirulencefactorordecoy AT petertimms theroleofthemultiplebandedantigenofureaplasmaparvuminintraamnioticinfectionmajorvirulencefactorordecoy AT christinelknox theroleofthemultiplebandedantigenofureaplasmaparvuminintraamnioticinfectionmajorvirulencefactorordecoy AT samanthajdando roleofthemultiplebandedantigenofureaplasmaparvuminintraamnioticinfectionmajorvirulencefactorordecoy AT iliasnitsos roleofthemultiplebandedantigenofureaplasmaparvuminintraamnioticinfectionmajorvirulencefactorordecoy AT suhasgkallapur roleofthemultiplebandedantigenofureaplasmaparvuminintraamnioticinfectionmajorvirulencefactorordecoy AT johnpnewnham roleofthemultiplebandedantigenofureaplasmaparvuminintraamnioticinfectionmajorvirulencefactorordecoy AT graemerpolglase roleofthemultiplebandedantigenofureaplasmaparvuminintraamnioticinfectionmajorvirulencefactorordecoy AT jjanepillow roleofthemultiplebandedantigenofureaplasmaparvuminintraamnioticinfectionmajorvirulencefactorordecoy AT alanhjobe roleofthemultiplebandedantigenofureaplasmaparvuminintraamnioticinfectionmajorvirulencefactorordecoy AT petertimms roleofthemultiplebandedantigenofureaplasmaparvuminintraamnioticinfectionmajorvirulencefactorordecoy AT christinelknox roleofthemultiplebandedantigenofureaplasmaparvuminintraamnioticinfectionmajorvirulencefactorordecoy |
| _version_ |
1718423358965547008 |