High resistance to Toxoplasma gondii infection in inducible nitric oxide synthase knockout rats

Summary: Nitric oxide (NO) is an important immune molecule that acts against extracellular and intracellular pathogens in most hosts. However, after the knockout of inducible nitric oxide synthase (iNOS−/−) in Sprague Dawley (SD) rats, these iNOS−/− rats were found to be completely resistant to Toxo...

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Autores principales:	Zhen-Jie Wang, Shao-Meng Yu, Jiang-Mei Gao, Peng Zhang, Geoff Hide, Masahiro Yamamoto, De-Hua Lai, Zhao-Rong Lun
Formato:	article
Lenguaje:	EN
Publicado:	Elsevier 2021
Materias:	Immunology Microbiology Parasitology Science Q
Acceso en línea:	https://doaj.org/article/8c9f5c074b0648d6a4527f5d3c947cc1
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spelling	oai:doaj.org-article:8c9f5c074b0648d6a4527f5d3c947cc12021-11-20T05:09:16ZHigh resistance to Toxoplasma gondii infection in inducible nitric oxide synthase knockout rats2589-004210.1016/j.isci.2021.103280https://doaj.org/article/8c9f5c074b0648d6a4527f5d3c947cc12021-11-01T00:00:00Zhttp://www.sciencedirect.com/science/article/pii/S2589004221012499https://doaj.org/toc/2589-0042Summary: Nitric oxide (NO) is an important immune molecule that acts against extracellular and intracellular pathogens in most hosts. However, after the knockout of inducible nitric oxide synthase (iNOS−/−) in Sprague Dawley (SD) rats, these iNOS−/− rats were found to be completely resistant to Toxoplasma gondii infection. Once the iNOS−/− rat peritoneal macrophages (PMs) were infected with T. gondii, they produced high levels of reactive oxygen species (ROS) triggered by GRA43 secreted by T. gondii, which damaged the parasitophorous vacuole membrane and PM mitochondrial membranes within a few hours post-infection. Further evidence indicated that the high levels of ROS caused mitochondrial superoxide dismutase 2 depletion and induced PM pyroptosis and cell death. This discovery of complete resistance to T. gondii infection, in the iNOS−/−-SD rat, demonstrates a strong link between NO and ROS in immunity to T. gondii infection and showcases a potentially novel and effective backup innate immunity system.Zhen-Jie WangShao-Meng YuJiang-Mei GaoPeng ZhangGeoff HideMasahiro YamamotoDe-Hua LaiZhao-Rong LunElsevierarticleImmunologyMicrobiologyParasitologyScienceQENiScience, Vol 24, Iss 11, Pp 103280- (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Immunology Microbiology Parasitology Science Q
spellingShingle	Immunology Microbiology Parasitology Science Q Zhen-Jie Wang Shao-Meng Yu Jiang-Mei Gao Peng Zhang Geoff Hide Masahiro Yamamoto De-Hua Lai Zhao-Rong Lun High resistance to Toxoplasma gondii infection in inducible nitric oxide synthase knockout rats
description	Summary: Nitric oxide (NO) is an important immune molecule that acts against extracellular and intracellular pathogens in most hosts. However, after the knockout of inducible nitric oxide synthase (iNOS−/−) in Sprague Dawley (SD) rats, these iNOS−/− rats were found to be completely resistant to Toxoplasma gondii infection. Once the iNOS−/− rat peritoneal macrophages (PMs) were infected with T. gondii, they produced high levels of reactive oxygen species (ROS) triggered by GRA43 secreted by T. gondii, which damaged the parasitophorous vacuole membrane and PM mitochondrial membranes within a few hours post-infection. Further evidence indicated that the high levels of ROS caused mitochondrial superoxide dismutase 2 depletion and induced PM pyroptosis and cell death. This discovery of complete resistance to T. gondii infection, in the iNOS−/−-SD rat, demonstrates a strong link between NO and ROS in immunity to T. gondii infection and showcases a potentially novel and effective backup innate immunity system.
format	article
author	Zhen-Jie Wang Shao-Meng Yu Jiang-Mei Gao Peng Zhang Geoff Hide Masahiro Yamamoto De-Hua Lai Zhao-Rong Lun
author_facet	Zhen-Jie Wang Shao-Meng Yu Jiang-Mei Gao Peng Zhang Geoff Hide Masahiro Yamamoto De-Hua Lai Zhao-Rong Lun
author_sort	Zhen-Jie Wang
title	High resistance to Toxoplasma gondii infection in inducible nitric oxide synthase knockout rats
title_short	High resistance to Toxoplasma gondii infection in inducible nitric oxide synthase knockout rats
title_full	High resistance to Toxoplasma gondii infection in inducible nitric oxide synthase knockout rats
title_fullStr	High resistance to Toxoplasma gondii infection in inducible nitric oxide synthase knockout rats
title_full_unstemmed	High resistance to Toxoplasma gondii infection in inducible nitric oxide synthase knockout rats
title_sort	high resistance to toxoplasma gondii infection in inducible nitric oxide synthase knockout rats
publisher	Elsevier
publishDate	2021
url	https://doaj.org/article/8c9f5c074b0648d6a4527f5d3c947cc1
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High resistance to Toxoplasma gondii infection in inducible nitric oxide synthase knockout rats

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