A Simple Exercise for Teaching Bacteriophage Concepts in the Undergraduate Laboratory Using Commercially Available Disinfectant

Virus and bacteriophage are key components in any microbiology teaching. We have used a commercially available disinfectant (TRIGENE) to demonstrate plaques formation from bacteriophages on confluent bacterial lawns. We have designed a laboratory exercise on phage typing using these plaques in a sin...

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Autores principales:	Latifa B. Khan, Hannah M. Read
Formato:	article
Lenguaje:	EN
Publicado:	American Society for Microbiology 2018
Materias:	Special aspects of education LC8-6691 Biology (General) QH301-705.5
Acceso en línea:	https://doaj.org/article/8ca564e01f654b2e936112a02e4e4c1f
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spelling	oai:doaj.org-article:8ca564e01f654b2e936112a02e4e4c1f2021-11-15T15:04:10ZA Simple Exercise for Teaching Bacteriophage Concepts in the Undergraduate Laboratory Using Commercially Available Disinfectant10.1128/jmbe.v19i2.15271935-78851935-7877https://doaj.org/article/8ca564e01f654b2e936112a02e4e4c1f2018-01-01T00:00:00Zhttps://journals.asm.org/doi/10.1128/jmbe.v19i2.1527https://doaj.org/toc/1935-7877https://doaj.org/toc/1935-7885Virus and bacteriophage are key components in any microbiology teaching. We have used a commercially available disinfectant (TRIGENE) to demonstrate plaques formation from bacteriophages on confluent bacterial lawns. We have designed a laboratory exercise on phage typing using these plaques in a single practical class to teach the concepts of bacteria-virus interactions, specificity, and diversity in a second year biomedical science course. The artificial bacteriophage-plaques are economical and capable of reliably mimicking bacteriophage effects. Use of the disinfectant to mimick bacteriophage effects eliminated the need to source and reproduce real bacteriophages, enabling fast preparation and demonstration of plaque assay in an undergraduate laboratory. Our phage typing teaching exercise facilitated effective students’ learning about bacteriophages with minimal laboratory skills or instructor intervention.Latifa B. KhanHannah M. ReadAmerican Society for MicrobiologyarticleSpecial aspects of educationLC8-6691Biology (General)QH301-705.5ENJournal of Microbiology & Biology Education, Vol 19, Iss 2 (2018)
institution	DOAJ
collection	DOAJ
language	EN
topic	Special aspects of education LC8-6691 Biology (General) QH301-705.5
spellingShingle	Special aspects of education LC8-6691 Biology (General) QH301-705.5 Latifa B. Khan Hannah M. Read A Simple Exercise for Teaching Bacteriophage Concepts in the Undergraduate Laboratory Using Commercially Available Disinfectant
description	Virus and bacteriophage are key components in any microbiology teaching. We have used a commercially available disinfectant (TRIGENE) to demonstrate plaques formation from bacteriophages on confluent bacterial lawns. We have designed a laboratory exercise on phage typing using these plaques in a single practical class to teach the concepts of bacteria-virus interactions, specificity, and diversity in a second year biomedical science course. The artificial bacteriophage-plaques are economical and capable of reliably mimicking bacteriophage effects. Use of the disinfectant to mimick bacteriophage effects eliminated the need to source and reproduce real bacteriophages, enabling fast preparation and demonstration of plaque assay in an undergraduate laboratory. Our phage typing teaching exercise facilitated effective students’ learning about bacteriophages with minimal laboratory skills or instructor intervention.
format	article
author	Latifa B. Khan Hannah M. Read
author_facet	Latifa B. Khan Hannah M. Read
author_sort	Latifa B. Khan
title	A Simple Exercise for Teaching Bacteriophage Concepts in the Undergraduate Laboratory Using Commercially Available Disinfectant
title_short	A Simple Exercise for Teaching Bacteriophage Concepts in the Undergraduate Laboratory Using Commercially Available Disinfectant
title_full	A Simple Exercise for Teaching Bacteriophage Concepts in the Undergraduate Laboratory Using Commercially Available Disinfectant
title_fullStr	A Simple Exercise for Teaching Bacteriophage Concepts in the Undergraduate Laboratory Using Commercially Available Disinfectant
title_full_unstemmed	A Simple Exercise for Teaching Bacteriophage Concepts in the Undergraduate Laboratory Using Commercially Available Disinfectant
title_sort	simple exercise for teaching bacteriophage concepts in the undergraduate laboratory using commercially available disinfectant
publisher	American Society for Microbiology
publishDate	2018
url	https://doaj.org/article/8ca564e01f654b2e936112a02e4e4c1f
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A Simple Exercise for Teaching Bacteriophage Concepts in the Undergraduate Laboratory Using Commercially Available Disinfectant

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