Multiplexed Cas9 targeting reveals genomic location effects and gRNA-based staggered breaks influencing mutation efficiency

Designing effective genome engineering strategies requires an understanding of the impact that genomic locus has on CRISPR-Cas9 activity. Here the authors use TRIP integrations to profile editing outcomes genome-wide and observe that gRNA sequence influences the structure of the double strand break.

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Bibliographic Details
Main Authors:	Santiago Gisler, Joana P. Gonçalves, Waseem Akhtar, Johann de Jong, Alexey V. Pindyurin, Lodewyk F. A. Wessels, Maarten van Lohuizen
Format:	article
Language:	EN
Published:	Nature Portfolio 2019
Subjects:	Science Q
Online Access:	https://doaj.org/article/8cafb83aa5a34b2b9543a0254a16b6e2
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spelling	oai:doaj.org-article:8cafb83aa5a34b2b9543a0254a16b6e22021-12-02T15:36:02ZMultiplexed Cas9 targeting reveals genomic location effects and gRNA-based staggered breaks influencing mutation efficiency10.1038/s41467-019-09551-w2041-1723https://doaj.org/article/8cafb83aa5a34b2b9543a0254a16b6e22019-04-01T00:00:00Zhttps://doi.org/10.1038/s41467-019-09551-whttps://doaj.org/toc/2041-1723Designing effective genome engineering strategies requires an understanding of the impact that genomic locus has on CRISPR-Cas9 activity. Here the authors use TRIP integrations to profile editing outcomes genome-wide and observe that gRNA sequence influences the structure of the double strand break.Santiago GislerJoana P. GonçalvesWaseem AkhtarJohann de JongAlexey V. PindyurinLodewyk F. A. WesselsMaarten van LohuizenNature PortfolioarticleScienceQENNature Communications, Vol 10, Iss 1, Pp 1-14 (2019)
institution	DOAJ
collection	DOAJ
language	EN
topic	Science Q
spellingShingle	Science Q Santiago Gisler Joana P. Gonçalves Waseem Akhtar Johann de Jong Alexey V. Pindyurin Lodewyk F. A. Wessels Maarten van Lohuizen Multiplexed Cas9 targeting reveals genomic location effects and gRNA-based staggered breaks influencing mutation efficiency
description	Designing effective genome engineering strategies requires an understanding of the impact that genomic locus has on CRISPR-Cas9 activity. Here the authors use TRIP integrations to profile editing outcomes genome-wide and observe that gRNA sequence influences the structure of the double strand break.
format	article
author	Santiago Gisler Joana P. Gonçalves Waseem Akhtar Johann de Jong Alexey V. Pindyurin Lodewyk F. A. Wessels Maarten van Lohuizen
author_facet	Santiago Gisler Joana P. Gonçalves Waseem Akhtar Johann de Jong Alexey V. Pindyurin Lodewyk F. A. Wessels Maarten van Lohuizen
author_sort	Santiago Gisler
title	Multiplexed Cas9 targeting reveals genomic location effects and gRNA-based staggered breaks influencing mutation efficiency
title_short	Multiplexed Cas9 targeting reveals genomic location effects and gRNA-based staggered breaks influencing mutation efficiency
title_full	Multiplexed Cas9 targeting reveals genomic location effects and gRNA-based staggered breaks influencing mutation efficiency
title_fullStr	Multiplexed Cas9 targeting reveals genomic location effects and gRNA-based staggered breaks influencing mutation efficiency
title_full_unstemmed	Multiplexed Cas9 targeting reveals genomic location effects and gRNA-based staggered breaks influencing mutation efficiency
title_sort	multiplexed cas9 targeting reveals genomic location effects and grna-based staggered breaks influencing mutation efficiency
publisher	Nature Portfolio
publishDate	2019
url	https://doaj.org/article/8cafb83aa5a34b2b9543a0254a16b6e2
work_keys_str_mv	AT santiagogisler multiplexedcas9targetingrevealsgenomiclocationeffectsandgrnabasedstaggeredbreaksinfluencingmutationefficiency AT joanapgoncalves multiplexedcas9targetingrevealsgenomiclocationeffectsandgrnabasedstaggeredbreaksinfluencingmutationefficiency AT waseemakhtar multiplexedcas9targetingrevealsgenomiclocationeffectsandgrnabasedstaggeredbreaksinfluencingmutationefficiency AT johanndejong multiplexedcas9targetingrevealsgenomiclocationeffectsandgrnabasedstaggeredbreaksinfluencingmutationefficiency AT alexeyvpindyurin multiplexedcas9targetingrevealsgenomiclocationeffectsandgrnabasedstaggeredbreaksinfluencingmutationefficiency AT lodewykfawessels multiplexedcas9targetingrevealsgenomiclocationeffectsandgrnabasedstaggeredbreaksinfluencingmutationefficiency AT maartenvanlohuizen multiplexedcas9targetingrevealsgenomiclocationeffectsandgrnabasedstaggeredbreaksinfluencingmutationefficiency
_version_	1718386366485626880

Multiplexed Cas9 targeting reveals genomic location effects and gRNA-based staggered breaks influencing mutation efficiency

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