Evaluation of Dried Blood Spots and Oral Fluids as Alternatives to Serum for Human Papillomavirus Antibody Surveillance

ABSTRACT Human papillomavirus (HPV) vaccination elicits high-titer genotype-specific antibody responses that are associated with a reduced risk of cervical disease caused by vaccine-incorporated genotypes. Our objective was to evaluate dried blood spots (DBSs) and oral mucosal transudate (OMT) as al...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Karly S. Louie, Jama Dalel, Caroline Reuter, Sara L. Bissett, Michelle Kleeman, Lesley Ashdown-Barr, Rawinder Banwait, Anna Godi, Peter Sasieni, Simon Beddows
Formato:	article
Lenguaje:	EN
Publicado:	American Society for Microbiology 2018
Materias:	HPV vaccine human papillomavirus antibodies dried blood spot oral fluids surveillance Microbiology QR1-502
Acceso en línea:	https://doaj.org/article/8cb1ae3346374cacbc738bdc0722231f
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:8cb1ae3346374cacbc738bdc0722231f
record_format	dspace
spelling	oai:doaj.org-article:8cb1ae3346374cacbc738bdc0722231f2021-11-15T15:24:23ZEvaluation of Dried Blood Spots and Oral Fluids as Alternatives to Serum for Human Papillomavirus Antibody Surveillance10.1128/mSphere.00043-182379-5042https://doaj.org/article/8cb1ae3346374cacbc738bdc0722231f2018-06-01T00:00:00Zhttps://journals.asm.org/doi/10.1128/mSphere.00043-18https://doaj.org/toc/2379-5042ABSTRACT Human papillomavirus (HPV) vaccination elicits high-titer genotype-specific antibody responses that are associated with a reduced risk of cervical disease caused by vaccine-incorporated genotypes. Our objective was to evaluate dried blood spots (DBSs) and oral mucosal transudate (OMT) as alternative samples to serum to confirm HPV vaccine antibody status. A study was carried out to evaluate the feasibility of detecting HPV16 and HPV18 antibodies in OMT, DBSs, and sera among women who self-reported being unvaccinated or fully vaccinated with the HPV vaccine. Serum had the highest sensitivity (100%) for detection of antibodies against both HPV16 and HPV18 but the lowest specificity, due to the detection of natural infection antibodies in 16% of unvaccinated women. Conversely, DBSs and OMT had lower sensitivity (96% and 82%, respectively) but high specificity (98%). We confirmed that these antibodies were functional (i.e., neutralizing) and that their detection was quantitatively reproducible and well correlated between sample types when normalized to IgG content. DBSs and OMT are appropriate alternative sample types for HPV vaccine surveillance. These alternative sample types warrant consideration for the purposes of cervical screening, diagnosis, and management, but more work will be needed to establish the stringent parameters required for such application. IMPORTANCE Human papillomavirus (HPV) is the causative agent of cervical and other anogenital cancers. HPV vaccination, primarily targeted at young girls before the age of sexual debut, is starting to demonstrate population-level declines in HPV infection and early disease associated with vaccine-incorporated genotypes. Monitoring young women for vaccine-specific antibody is important for vaccine surveillance and may be useful as an adjunct test within a cervical screening context. We evaluated serum, dried blood spots, and oral fluid as potential samples for such applications and report robust measures of diagnostic accuracy. This is the first time a direct comparison of alternative sample types has been made between vaccinated and unvaccinated women for the detection and quantitation of HPV antibodies.Karly S. LouieJama DalelCaroline ReuterSara L. BissettMichelle KleemanLesley Ashdown-BarrRawinder BanwaitAnna GodiPeter SasieniSimon BeddowsAmerican Society for MicrobiologyarticleHPV vaccinehuman papillomavirusantibodiesdried blood spotoral fluidssurveillanceMicrobiologyQR1-502ENmSphere, Vol 3, Iss 3 (2018)
institution	DOAJ
collection	DOAJ
language	EN
topic	HPV vaccine human papillomavirus antibodies dried blood spot oral fluids surveillance Microbiology QR1-502
spellingShingle	HPV vaccine human papillomavirus antibodies dried blood spot oral fluids surveillance Microbiology QR1-502 Karly S. Louie Jama Dalel Caroline Reuter Sara L. Bissett Michelle Kleeman Lesley Ashdown-Barr Rawinder Banwait Anna Godi Peter Sasieni Simon Beddows Evaluation of Dried Blood Spots and Oral Fluids as Alternatives to Serum for Human Papillomavirus Antibody Surveillance
description	ABSTRACT Human papillomavirus (HPV) vaccination elicits high-titer genotype-specific antibody responses that are associated with a reduced risk of cervical disease caused by vaccine-incorporated genotypes. Our objective was to evaluate dried blood spots (DBSs) and oral mucosal transudate (OMT) as alternative samples to serum to confirm HPV vaccine antibody status. A study was carried out to evaluate the feasibility of detecting HPV16 and HPV18 antibodies in OMT, DBSs, and sera among women who self-reported being unvaccinated or fully vaccinated with the HPV vaccine. Serum had the highest sensitivity (100%) for detection of antibodies against both HPV16 and HPV18 but the lowest specificity, due to the detection of natural infection antibodies in 16% of unvaccinated women. Conversely, DBSs and OMT had lower sensitivity (96% and 82%, respectively) but high specificity (98%). We confirmed that these antibodies were functional (i.e., neutralizing) and that their detection was quantitatively reproducible and well correlated between sample types when normalized to IgG content. DBSs and OMT are appropriate alternative sample types for HPV vaccine surveillance. These alternative sample types warrant consideration for the purposes of cervical screening, diagnosis, and management, but more work will be needed to establish the stringent parameters required for such application. IMPORTANCE Human papillomavirus (HPV) is the causative agent of cervical and other anogenital cancers. HPV vaccination, primarily targeted at young girls before the age of sexual debut, is starting to demonstrate population-level declines in HPV infection and early disease associated with vaccine-incorporated genotypes. Monitoring young women for vaccine-specific antibody is important for vaccine surveillance and may be useful as an adjunct test within a cervical screening context. We evaluated serum, dried blood spots, and oral fluid as potential samples for such applications and report robust measures of diagnostic accuracy. This is the first time a direct comparison of alternative sample types has been made between vaccinated and unvaccinated women for the detection and quantitation of HPV antibodies.
format	article
author	Karly S. Louie Jama Dalel Caroline Reuter Sara L. Bissett Michelle Kleeman Lesley Ashdown-Barr Rawinder Banwait Anna Godi Peter Sasieni Simon Beddows
author_facet	Karly S. Louie Jama Dalel Caroline Reuter Sara L. Bissett Michelle Kleeman Lesley Ashdown-Barr Rawinder Banwait Anna Godi Peter Sasieni Simon Beddows
author_sort	Karly S. Louie
title	Evaluation of Dried Blood Spots and Oral Fluids as Alternatives to Serum for Human Papillomavirus Antibody Surveillance
title_short	Evaluation of Dried Blood Spots and Oral Fluids as Alternatives to Serum for Human Papillomavirus Antibody Surveillance
title_full	Evaluation of Dried Blood Spots and Oral Fluids as Alternatives to Serum for Human Papillomavirus Antibody Surveillance
title_fullStr	Evaluation of Dried Blood Spots and Oral Fluids as Alternatives to Serum for Human Papillomavirus Antibody Surveillance
title_full_unstemmed	Evaluation of Dried Blood Spots and Oral Fluids as Alternatives to Serum for Human Papillomavirus Antibody Surveillance
title_sort	evaluation of dried blood spots and oral fluids as alternatives to serum for human papillomavirus antibody surveillance
publisher	American Society for Microbiology
publishDate	2018
url	https://doaj.org/article/8cb1ae3346374cacbc738bdc0722231f
work_keys_str_mv	AT karlyslouie evaluationofdriedbloodspotsandoralfluidsasalternativestoserumforhumanpapillomavirusantibodysurveillance AT jamadalel evaluationofdriedbloodspotsandoralfluidsasalternativestoserumforhumanpapillomavirusantibodysurveillance AT carolinereuter evaluationofdriedbloodspotsandoralfluidsasalternativestoserumforhumanpapillomavirusantibodysurveillance AT saralbissett evaluationofdriedbloodspotsandoralfluidsasalternativestoserumforhumanpapillomavirusantibodysurveillance AT michellekleeman evaluationofdriedbloodspotsandoralfluidsasalternativestoserumforhumanpapillomavirusantibodysurveillance AT lesleyashdownbarr evaluationofdriedbloodspotsandoralfluidsasalternativestoserumforhumanpapillomavirusantibodysurveillance AT rawinderbanwait evaluationofdriedbloodspotsandoralfluidsasalternativestoserumforhumanpapillomavirusantibodysurveillance AT annagodi evaluationofdriedbloodspotsandoralfluidsasalternativestoserumforhumanpapillomavirusantibodysurveillance AT petersasieni evaluationofdriedbloodspotsandoralfluidsasalternativestoserumforhumanpapillomavirusantibodysurveillance AT simonbeddows evaluationofdriedbloodspotsandoralfluidsasalternativestoserumforhumanpapillomavirusantibodysurveillance
_version_	1718427963371814912

Evaluation of Dried Blood Spots and Oral Fluids as Alternatives to Serum for Human Papillomavirus Antibody Surveillance

Ejemplares similares