Magnetic-nanoparticles-based fluorescent immunoassay for individual and simultaneous determination of dichlorvos and paraoxon in milk

A sensitive and rapid magnetic-nanoparticles-based fluorescent immunoassay (MNPs-FIA) for individual and simultaneous determination of dichlorvos (DDVP) and paraoxon in untreated raw milk was developed. The conjugates DDVP–cBSA (cationized bovine serum albumin) and paraoxon–BSA (bovine serum albumin...

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Autores principales:	Marina Yaneva, Yavor Ivanov, Nikola Todorov, Tzonka Godjevargova
Formato:	article
Lenguaje:	EN
Publicado:	Taylor & Francis Group 2018
Materias:	paraoxоn dichlorvos milk immunoassay fluorescence magnetic nanoparticles Agriculture (General) S1-972 Immunologic diseases. Allergy RC581-607
Acceso en línea:	https://doaj.org/article/8cb743bae03649ba8f774f34862a4972
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spelling	oai:doaj.org-article:8cb743bae03649ba8f774f34862a49722021-11-26T11:19:46ZMagnetic-nanoparticles-based fluorescent immunoassay for individual and simultaneous determination of dichlorvos and paraoxon in milk0954-01051465-344310.1080/09540105.2017.1368458https://doaj.org/article/8cb743bae03649ba8f774f34862a49722018-01-01T00:00:00Zhttp://dx.doi.org/10.1080/09540105.2017.1368458https://doaj.org/toc/0954-0105https://doaj.org/toc/1465-3443A sensitive and rapid magnetic-nanoparticles-based fluorescent immunoassay (MNPs-FIA) for individual and simultaneous determination of dichlorvos (DDVP) and paraoxon in untreated raw milk was developed. The conjugates DDVP–cBSA (cationized bovine serum albumin) and paraoxon–BSA (bovine serum albumin) were labeled by fluorescent dyes. The optimal amount of immobilized polyclonal anti-DDVP and anti-paraoxon antibodies on MNPs (0.375 mg Ab-MNPs) and optimal concentrations of DDVP–cBSA–FITC (18 µg/mL) and paraoxon–BSA–FITC (22 µg/mL) for MNPs-FIA were determined. The calibration curves of individual and simultaneous immunoassays of DDVP and paraoxon were investigated. The IC50 value of the individual paraoxon assay in raw cow’s milk was 60 ng/mL and linear range 2–300 ng/mL. The IC50 value of the individual DDVP assay was 70 ng/mL and linear range 5–300 ng/mL. The IC50 values of the paraoxon and DDVP (100 and 120 ng/mL respectively) in simultaneous assay were higher than those of the individual ones, and their linear ranges were 10–400 ng/mL.Marina YanevaYavor IvanovNikola TodorovTzonka GodjevargovaTaylor & Francis Grouparticleparaoxоndichlorvosmilkimmunoassayfluorescencemagnetic nanoparticlesAgriculture (General)S1-972Immunologic diseases. AllergyRC581-607ENFood and Agricultural Immunology, Vol 29, Iss 1, Pp 228-243 (2018)
institution	DOAJ
collection	DOAJ
language	EN
topic	paraoxоn dichlorvos milk immunoassay fluorescence magnetic nanoparticles Agriculture (General) S1-972 Immunologic diseases. Allergy RC581-607
spellingShingle	paraoxоn dichlorvos milk immunoassay fluorescence magnetic nanoparticles Agriculture (General) S1-972 Immunologic diseases. Allergy RC581-607 Marina Yaneva Yavor Ivanov Nikola Todorov Tzonka Godjevargova Magnetic-nanoparticles-based fluorescent immunoassay for individual and simultaneous determination of dichlorvos and paraoxon in milk
description	A sensitive and rapid magnetic-nanoparticles-based fluorescent immunoassay (MNPs-FIA) for individual and simultaneous determination of dichlorvos (DDVP) and paraoxon in untreated raw milk was developed. The conjugates DDVP–cBSA (cationized bovine serum albumin) and paraoxon–BSA (bovine serum albumin) were labeled by fluorescent dyes. The optimal amount of immobilized polyclonal anti-DDVP and anti-paraoxon antibodies on MNPs (0.375 mg Ab-MNPs) and optimal concentrations of DDVP–cBSA–FITC (18 µg/mL) and paraoxon–BSA–FITC (22 µg/mL) for MNPs-FIA were determined. The calibration curves of individual and simultaneous immunoassays of DDVP and paraoxon were investigated. The IC50 value of the individual paraoxon assay in raw cow’s milk was 60 ng/mL and linear range 2–300 ng/mL. The IC50 value of the individual DDVP assay was 70 ng/mL and linear range 5–300 ng/mL. The IC50 values of the paraoxon and DDVP (100 and 120 ng/mL respectively) in simultaneous assay were higher than those of the individual ones, and their linear ranges were 10–400 ng/mL.
format	article
author	Marina Yaneva Yavor Ivanov Nikola Todorov Tzonka Godjevargova
author_facet	Marina Yaneva Yavor Ivanov Nikola Todorov Tzonka Godjevargova
author_sort	Marina Yaneva
title	Magnetic-nanoparticles-based fluorescent immunoassay for individual and simultaneous determination of dichlorvos and paraoxon in milk
title_short	Magnetic-nanoparticles-based fluorescent immunoassay for individual and simultaneous determination of dichlorvos and paraoxon in milk
title_full	Magnetic-nanoparticles-based fluorescent immunoassay for individual and simultaneous determination of dichlorvos and paraoxon in milk
title_fullStr	Magnetic-nanoparticles-based fluorescent immunoassay for individual and simultaneous determination of dichlorvos and paraoxon in milk
title_full_unstemmed	Magnetic-nanoparticles-based fluorescent immunoassay for individual and simultaneous determination of dichlorvos and paraoxon in milk
title_sort	magnetic-nanoparticles-based fluorescent immunoassay for individual and simultaneous determination of dichlorvos and paraoxon in milk
publisher	Taylor & Francis Group
publishDate	2018
url	https://doaj.org/article/8cb743bae03649ba8f774f34862a4972
work_keys_str_mv	AT marinayaneva magneticnanoparticlesbasedfluorescentimmunoassayforindividualandsimultaneousdeterminationofdichlorvosandparaoxoninmilk AT yavorivanov magneticnanoparticlesbasedfluorescentimmunoassayforindividualandsimultaneousdeterminationofdichlorvosandparaoxoninmilk AT nikolatodorov magneticnanoparticlesbasedfluorescentimmunoassayforindividualandsimultaneousdeterminationofdichlorvosandparaoxoninmilk AT tzonkagodjevargova magneticnanoparticlesbasedfluorescentimmunoassayforindividualandsimultaneousdeterminationofdichlorvosandparaoxoninmilk
_version_	1718409547178049536

Magnetic-nanoparticles-based fluorescent immunoassay for individual and simultaneous determination of dichlorvos and paraoxon in milk

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