Alginate-Capped Silver Nanoparticles as a Potent Anti-mycobacterial Agent Against Mycobacterium tuberculosis

Tuberculosis (TB) is a leading cause of death from a single infectious agent, Mycobacterium tuberculosis (Mtb). Although progress has been made in TB control, still about 10 million people worldwide develop TB annually and 1.5 million die of the disease. The rapid emergence of aggressive, drug-resis...

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Autores principales:	Cheng-Cheung Chen, Yih-Yuan Chen, Chang-Ching Yeh, Chia-Wei Hsu, Shang-Jie Yu, Chih-Hao Hsu, Ting-Chun Wei, Sin-Ni Ho, Pei-Chu Tsai, Yung-Deng Song, Hui-Ju Yen, Xin-An Chen, Jenn-Jong Young, Chuan-Chung Chuang, Horng-Yunn Dou
Formato:	article
Lenguaje:	EN
Publicado:	Frontiers Media S.A. 2021
Materias:	mycobacterium tubeculosis antimycobacterial agent MDR TB dormant TB silver nanopaiticles Therapeutics. Pharmacology RM1-950
Acceso en línea:	https://doaj.org/article/8cbd6e4b0df74eb685a41fc17de84b10
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spelling	oai:doaj.org-article:8cbd6e4b0df74eb685a41fc17de84b102021-11-17T05:56:55ZAlginate-Capped Silver Nanoparticles as a Potent Anti-mycobacterial Agent Against Mycobacterium tuberculosis1663-981210.3389/fphar.2021.746496https://doaj.org/article/8cbd6e4b0df74eb685a41fc17de84b102021-11-01T00:00:00Zhttps://www.frontiersin.org/articles/10.3389/fphar.2021.746496/fullhttps://doaj.org/toc/1663-9812Tuberculosis (TB) is a leading cause of death from a single infectious agent, Mycobacterium tuberculosis (Mtb). Although progress has been made in TB control, still about 10 million people worldwide develop TB annually and 1.5 million die of the disease. The rapid emergence of aggressive, drug-resistant strains and latent infections have caused TB to remain a global health challenge. TB treatments are lengthy and their side effects lead to poor patient compliance, which in turn has contributed to the drug resistance and exacerbated the TB epidemic. The relatively low output of newly approved antibiotics has spurred research interest toward alternative antibacterial molecules such as silver nanoparticles (AgNPs). In the present study, we use the natural biopolymer alginate to serve as a stabilizer and/or reductant to green synthesize AgNPs, which improves their biocompatibility and avoids the use of toxic chemicals. The average size of the alginate-capped AgNPs (ALG-AgNPs) was characterized as nanoscale, and the particles were round in shape. Drug susceptibility tests showed that these ALG-AgNPs are effective against both drug-resistant Mtb strains and dormant Mtb. A bacterial cell-wall permeability assay showed that the anti-mycobacterial action of ALG-AgNPs is mediated through an increase in cell-wall permeability. Notably, the anti-mycobacterial potential of ALG-AgNPs was effective in both zebrafish and mouse TB animal models in vivo. These results suggest that ALG-AgNPs could provide a new therapeutic option to overcome the difficulties of current TB treatments.Cheng-Cheung ChenCheng-Cheung ChenYih-Yuan ChenChang-Ching YehChia-Wei HsuShang-Jie YuChih-Hao HsuTing-Chun WeiSin-Ni HoPei-Chu TsaiYung-Deng SongHui-Ju YenHui-Ju YenXin-An ChenJenn-Jong YoungChuan-Chung ChuangChuan-Chung ChuangHorng-Yunn DouHorng-Yunn DouFrontiers Media S.A.articlemycobacterium tubeculosisantimycobacterial agentMDR TBdormant TBsilver nanopaiticlesTherapeutics. PharmacologyRM1-950ENFrontiers in Pharmacology, Vol 12 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	mycobacterium tubeculosis antimycobacterial agent MDR TB dormant TB silver nanopaiticles Therapeutics. Pharmacology RM1-950
spellingShingle	mycobacterium tubeculosis antimycobacterial agent MDR TB dormant TB silver nanopaiticles Therapeutics. Pharmacology RM1-950 Cheng-Cheung Chen Cheng-Cheung Chen Yih-Yuan Chen Chang-Ching Yeh Chia-Wei Hsu Shang-Jie Yu Chih-Hao Hsu Ting-Chun Wei Sin-Ni Ho Pei-Chu Tsai Yung-Deng Song Hui-Ju Yen Hui-Ju Yen Xin-An Chen Jenn-Jong Young Chuan-Chung Chuang Chuan-Chung Chuang Horng-Yunn Dou Horng-Yunn Dou Alginate-Capped Silver Nanoparticles as a Potent Anti-mycobacterial Agent Against Mycobacterium tuberculosis
description	Tuberculosis (TB) is a leading cause of death from a single infectious agent, Mycobacterium tuberculosis (Mtb). Although progress has been made in TB control, still about 10 million people worldwide develop TB annually and 1.5 million die of the disease. The rapid emergence of aggressive, drug-resistant strains and latent infections have caused TB to remain a global health challenge. TB treatments are lengthy and their side effects lead to poor patient compliance, which in turn has contributed to the drug resistance and exacerbated the TB epidemic. The relatively low output of newly approved antibiotics has spurred research interest toward alternative antibacterial molecules such as silver nanoparticles (AgNPs). In the present study, we use the natural biopolymer alginate to serve as a stabilizer and/or reductant to green synthesize AgNPs, which improves their biocompatibility and avoids the use of toxic chemicals. The average size of the alginate-capped AgNPs (ALG-AgNPs) was characterized as nanoscale, and the particles were round in shape. Drug susceptibility tests showed that these ALG-AgNPs are effective against both drug-resistant Mtb strains and dormant Mtb. A bacterial cell-wall permeability assay showed that the anti-mycobacterial action of ALG-AgNPs is mediated through an increase in cell-wall permeability. Notably, the anti-mycobacterial potential of ALG-AgNPs was effective in both zebrafish and mouse TB animal models in vivo. These results suggest that ALG-AgNPs could provide a new therapeutic option to overcome the difficulties of current TB treatments.
format	article
author	Cheng-Cheung Chen Cheng-Cheung Chen Yih-Yuan Chen Chang-Ching Yeh Chia-Wei Hsu Shang-Jie Yu Chih-Hao Hsu Ting-Chun Wei Sin-Ni Ho Pei-Chu Tsai Yung-Deng Song Hui-Ju Yen Hui-Ju Yen Xin-An Chen Jenn-Jong Young Chuan-Chung Chuang Chuan-Chung Chuang Horng-Yunn Dou Horng-Yunn Dou
author_facet	Cheng-Cheung Chen Cheng-Cheung Chen Yih-Yuan Chen Chang-Ching Yeh Chia-Wei Hsu Shang-Jie Yu Chih-Hao Hsu Ting-Chun Wei Sin-Ni Ho Pei-Chu Tsai Yung-Deng Song Hui-Ju Yen Hui-Ju Yen Xin-An Chen Jenn-Jong Young Chuan-Chung Chuang Chuan-Chung Chuang Horng-Yunn Dou Horng-Yunn Dou
author_sort	Cheng-Cheung Chen
title	Alginate-Capped Silver Nanoparticles as a Potent Anti-mycobacterial Agent Against Mycobacterium tuberculosis
title_short	Alginate-Capped Silver Nanoparticles as a Potent Anti-mycobacterial Agent Against Mycobacterium tuberculosis
title_full	Alginate-Capped Silver Nanoparticles as a Potent Anti-mycobacterial Agent Against Mycobacterium tuberculosis
title_fullStr	Alginate-Capped Silver Nanoparticles as a Potent Anti-mycobacterial Agent Against Mycobacterium tuberculosis
title_full_unstemmed	Alginate-Capped Silver Nanoparticles as a Potent Anti-mycobacterial Agent Against Mycobacterium tuberculosis
title_sort	alginate-capped silver nanoparticles as a potent anti-mycobacterial agent against mycobacterium tuberculosis
publisher	Frontiers Media S.A.
publishDate	2021
url	https://doaj.org/article/8cbd6e4b0df74eb685a41fc17de84b10
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Alginate-Capped Silver Nanoparticles as a Potent Anti-mycobacterial Agent Against Mycobacterium tuberculosis

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