Versatile artificial mer operons in Escherichia coli towards whole cell biosensing and adsorption of mercury.

Mercury exists naturally and mainly as a man-made pollutant in the environment, where it exerts adverse effects on local ecosystems and living organisms. It is important to develop an appropriate synthetic biological device that recognizes, detects and removes the bioavailable fraction of environmen...

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Autores principales:	Nai-Xing Zhang, Yan Guo, Hui Li, Xue-Qin Yang, Chao-Xian Gao, Chang-Ye Hui
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/8cbef97d2af2468c89912ca4211e3737
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spelling	oai:doaj.org-article:8cbef97d2af2468c89912ca4211e37372021-12-02T20:05:28ZVersatile artificial mer operons in Escherichia coli towards whole cell biosensing and adsorption of mercury.1932-620310.1371/journal.pone.0252190https://doaj.org/article/8cbef97d2af2468c89912ca4211e37372021-01-01T00:00:00Zhttps://doi.org/10.1371/journal.pone.0252190https://doaj.org/toc/1932-6203Mercury exists naturally and mainly as a man-made pollutant in the environment, where it exerts adverse effects on local ecosystems and living organisms. It is important to develop an appropriate synthetic biological device that recognizes, detects and removes the bioavailable fraction of environmental mercury. Both single-signal and double-signal output mercury biosensors were assembled using a natural mer operon as a template. Selectivity and sensitivity of whole-cell biosensors based on artificial mer operons were determined. Three whole-cell biosensors were highly stable at very high concentrations of mercuric chloride, and could detect bioavailable Hg(II) in the concentration range of 6.25-200 μM HgCl2. A novel Hg(II) bioadsorption coupled with biosensing artificial mer operon was assembled. This would allow Hg(II)-induced Hg(II) binding protein cell surface display and green fluorescence emission to be achieved simultaneously while retaining the linear relationship between fluorescent signal and Hg(II) exposure concentration. The present study provides an innovative way to simultaneously detect, quantify, and remove bioavailable heavy metal ions using an artificially reconstructed heavy metal resistance operon.Nai-Xing ZhangYan GuoHui LiXue-Qin YangChao-Xian GaoChang-Ye HuiPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 16, Iss 5, p e0252190 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Nai-Xing Zhang Yan Guo Hui Li Xue-Qin Yang Chao-Xian Gao Chang-Ye Hui Versatile artificial mer operons in Escherichia coli towards whole cell biosensing and adsorption of mercury.
description	Mercury exists naturally and mainly as a man-made pollutant in the environment, where it exerts adverse effects on local ecosystems and living organisms. It is important to develop an appropriate synthetic biological device that recognizes, detects and removes the bioavailable fraction of environmental mercury. Both single-signal and double-signal output mercury biosensors were assembled using a natural mer operon as a template. Selectivity and sensitivity of whole-cell biosensors based on artificial mer operons were determined. Three whole-cell biosensors were highly stable at very high concentrations of mercuric chloride, and could detect bioavailable Hg(II) in the concentration range of 6.25-200 μM HgCl2. A novel Hg(II) bioadsorption coupled with biosensing artificial mer operon was assembled. This would allow Hg(II)-induced Hg(II) binding protein cell surface display and green fluorescence emission to be achieved simultaneously while retaining the linear relationship between fluorescent signal and Hg(II) exposure concentration. The present study provides an innovative way to simultaneously detect, quantify, and remove bioavailable heavy metal ions using an artificially reconstructed heavy metal resistance operon.
format	article
author	Nai-Xing Zhang Yan Guo Hui Li Xue-Qin Yang Chao-Xian Gao Chang-Ye Hui
author_facet	Nai-Xing Zhang Yan Guo Hui Li Xue-Qin Yang Chao-Xian Gao Chang-Ye Hui
author_sort	Nai-Xing Zhang
title	Versatile artificial mer operons in Escherichia coli towards whole cell biosensing and adsorption of mercury.
title_short	Versatile artificial mer operons in Escherichia coli towards whole cell biosensing and adsorption of mercury.
title_full	Versatile artificial mer operons in Escherichia coli towards whole cell biosensing and adsorption of mercury.
title_fullStr	Versatile artificial mer operons in Escherichia coli towards whole cell biosensing and adsorption of mercury.
title_full_unstemmed	Versatile artificial mer operons in Escherichia coli towards whole cell biosensing and adsorption of mercury.
title_sort	versatile artificial mer operons in escherichia coli towards whole cell biosensing and adsorption of mercury.
publisher	Public Library of Science (PLoS)
publishDate	2021
url	https://doaj.org/article/8cbef97d2af2468c89912ca4211e3737
work_keys_str_mv	AT naixingzhang versatileartificialmeroperonsinescherichiacolitowardswholecellbiosensingandadsorptionofmercury AT yanguo versatileartificialmeroperonsinescherichiacolitowardswholecellbiosensingandadsorptionofmercury AT huili versatileartificialmeroperonsinescherichiacolitowardswholecellbiosensingandadsorptionofmercury AT xueqinyang versatileartificialmeroperonsinescherichiacolitowardswholecellbiosensingandadsorptionofmercury AT chaoxiangao versatileartificialmeroperonsinescherichiacolitowardswholecellbiosensingandadsorptionofmercury AT changyehui versatileartificialmeroperonsinescherichiacolitowardswholecellbiosensingandadsorptionofmercury
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Versatile artificial mer operons in Escherichia coli towards whole cell biosensing and adsorption of mercury.

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