VEGF siRNA delivered by polycation liposome-encapsulated calcium phosphate nanoparticles for tumor angiogenesis inhibition in breast cancer

Jinliang Chen,1 Xiaoyi Sun,2 Rong Shao,1 Yichao Xu,1 Jianqing Gao,3 Wenquan Liang3 1Center of Clinical Pharmacology, The Second Affiliated Hospital of Zhejiang University, School of Medicine, 2Department of Pharmacy, Zhejiang University City College, 3Institute of Pharmaceutics, College of Pharmace...

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Autores principales:	Chen J, Sun X, Shao R, Xu Y, Gao J, Liang W
Formato:	article
Lenguaje:	EN
Publicado:	Dove Medical Press 2017
Materias:	polycation liposomes calcium phosphate nanoparticles VEGF siRNA angiogenesis inhibition Medicine (General) R5-920
Acceso en línea:	https://doaj.org/article/8cc4215d4eb749169dd483eadb493b28
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spelling	oai:doaj.org-article:8cc4215d4eb749169dd483eadb493b282021-12-02T04:28:15ZVEGF siRNA delivered by polycation liposome-encapsulated calcium phosphate nanoparticles for tumor angiogenesis inhibition in breast cancer1178-2013https://doaj.org/article/8cc4215d4eb749169dd483eadb493b282017-08-01T00:00:00Zhttps://www.dovepress.com/vegf-sirna-delivered-by-polycation--liposome-encapsulated-calcium-phos-peer-reviewed-article-IJNhttps://doaj.org/toc/1178-2013Jinliang Chen,1 Xiaoyi Sun,2 Rong Shao,1 Yichao Xu,1 Jianqing Gao,3 Wenquan Liang3 1Center of Clinical Pharmacology, The Second Affiliated Hospital of Zhejiang University, School of Medicine, 2Department of Pharmacy, Zhejiang University City College, 3Institute of Pharmaceutics, College of Pharmaceutical Sciences, Zhejiang University, Hangzhou, Zhejiang, People’s Republic of China Abstract: Angiogenesis plays an important role in tumor development and metastasis, and many cancer cells upregulate VEGF expression to promote angiogenesis. Silencing VEGF expression by RNA interference is expected to be a promising strategy to suppress the tumor growth. However, low transfection efficiency and instability are the main barriers for small interfering RNA (siRNA) delivery. In this study, we developed polycation liposome-encapsulated calcium phosphate nanoparticles (PLCP) for siRNA delivery in vivo. VEGF expression silencing effect in MCF-7 cells was investigated by real-time quantitative polymerase chain reaction and Western blot assay. VEGF siRNA mediated by PLCP can reduce 60%–80% VEGF expression in vitro, which was significantly higher than that mediated by Lipofectamine 2000. Furthermore, significant tumor growth and angiogenesis inhibition were observed in MCF-7 xenografts mice when treated with PLCP/VEGF siRNA or combined with doxorubicin. In conclusion, the combination of silencing VEGF expression and chemotherapeutics would be a potential treatment for cancer therapy. Keywords: polycation liposomes, calcium phosphate nanoparticles, VEGF, siRNA, angiogenesis inhibition Chen JSun XShao RXu YGao JLiang WDove Medical Pressarticlepolycation liposomescalcium phosphate nanoparticlesVEGFsiRNAangiogenesis inhibitionMedicine (General)R5-920ENInternational Journal of Nanomedicine, Vol Volume 12, Pp 6075-6088 (2017)
institution	DOAJ
collection	DOAJ
language	EN
topic	polycation liposomes calcium phosphate nanoparticles VEGF siRNA angiogenesis inhibition Medicine (General) R5-920
spellingShingle	polycation liposomes calcium phosphate nanoparticles VEGF siRNA angiogenesis inhibition Medicine (General) R5-920 Chen J Sun X Shao R Xu Y Gao J Liang W VEGF siRNA delivered by polycation liposome-encapsulated calcium phosphate nanoparticles for tumor angiogenesis inhibition in breast cancer
description	Jinliang Chen,1 Xiaoyi Sun,2 Rong Shao,1 Yichao Xu,1 Jianqing Gao,3 Wenquan Liang3 1Center of Clinical Pharmacology, The Second Affiliated Hospital of Zhejiang University, School of Medicine, 2Department of Pharmacy, Zhejiang University City College, 3Institute of Pharmaceutics, College of Pharmaceutical Sciences, Zhejiang University, Hangzhou, Zhejiang, People’s Republic of China Abstract: Angiogenesis plays an important role in tumor development and metastasis, and many cancer cells upregulate VEGF expression to promote angiogenesis. Silencing VEGF expression by RNA interference is expected to be a promising strategy to suppress the tumor growth. However, low transfection efficiency and instability are the main barriers for small interfering RNA (siRNA) delivery. In this study, we developed polycation liposome-encapsulated calcium phosphate nanoparticles (PLCP) for siRNA delivery in vivo. VEGF expression silencing effect in MCF-7 cells was investigated by real-time quantitative polymerase chain reaction and Western blot assay. VEGF siRNA mediated by PLCP can reduce 60%–80% VEGF expression in vitro, which was significantly higher than that mediated by Lipofectamine 2000. Furthermore, significant tumor growth and angiogenesis inhibition were observed in MCF-7 xenografts mice when treated with PLCP/VEGF siRNA or combined with doxorubicin. In conclusion, the combination of silencing VEGF expression and chemotherapeutics would be a potential treatment for cancer therapy. Keywords: polycation liposomes, calcium phosphate nanoparticles, VEGF, siRNA, angiogenesis inhibition
format	article
author	Chen J Sun X Shao R Xu Y Gao J Liang W
author_facet	Chen J Sun X Shao R Xu Y Gao J Liang W
author_sort	Chen J
title	VEGF siRNA delivered by polycation liposome-encapsulated calcium phosphate nanoparticles for tumor angiogenesis inhibition in breast cancer
title_short	VEGF siRNA delivered by polycation liposome-encapsulated calcium phosphate nanoparticles for tumor angiogenesis inhibition in breast cancer
title_full	VEGF siRNA delivered by polycation liposome-encapsulated calcium phosphate nanoparticles for tumor angiogenesis inhibition in breast cancer
title_fullStr	VEGF siRNA delivered by polycation liposome-encapsulated calcium phosphate nanoparticles for tumor angiogenesis inhibition in breast cancer
title_full_unstemmed	VEGF siRNA delivered by polycation liposome-encapsulated calcium phosphate nanoparticles for tumor angiogenesis inhibition in breast cancer
title_sort	vegf sirna delivered by polycation liposome-encapsulated calcium phosphate nanoparticles for tumor angiogenesis inhibition in breast cancer
publisher	Dove Medical Press
publishDate	2017
url	https://doaj.org/article/8cc4215d4eb749169dd483eadb493b28
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VEGF siRNA delivered by polycation liposome-encapsulated calcium phosphate nanoparticles for tumor angiogenesis inhibition in breast cancer

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