A short artificial antimicrobial peptide shows potential to prevent or treat bone infections

Abstract Infection of bone is a severe complication due to the variety of bacteria causing it, their resistance against classical antibiotics, the formation of a biofilm and the difficulty to eradicate it. Antimicrobial peptides (AMPs) are naturally occurring peptides and promising candidates for tr...

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Autores principales:	N. Bormann, A. Koliszak, S. Kasper, L. Schoen, K. Hilpert, R. Volkmer, J. Kikhney, B. Wildemann
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2017
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/8cc49451c0b64c579e355aeb8941cf1a
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spelling	oai:doaj.org-article:8cc49451c0b64c579e355aeb8941cf1a2021-12-02T15:06:21ZA short artificial antimicrobial peptide shows potential to prevent or treat bone infections10.1038/s41598-017-01698-02045-2322https://doaj.org/article/8cc49451c0b64c579e355aeb8941cf1a2017-05-01T00:00:00Zhttps://doi.org/10.1038/s41598-017-01698-0https://doaj.org/toc/2045-2322Abstract Infection of bone is a severe complication due to the variety of bacteria causing it, their resistance against classical antibiotics, the formation of a biofilm and the difficulty to eradicate it. Antimicrobial peptides (AMPs) are naturally occurring peptides and promising candidates for treatment of joint infections. This study aimed to analyze the effect of short artificial peptides derived from an optimized library regarding (1) antimicrobial effect on different bacterial species, (2) efficacy on biofilms, and (3) effect on osteoblast‑like cells. Culturing the AMP-modifications with Escherichia coli, Enterococcus faecalis, Pseudomonas aeruginosa, Staphylococcus aureus (including clinical isolates of MRSA and MSSA) and Staphylococcus epidermidis identified one candidate that was most effective against all bacteria. This AMP was also able to reduce biofilm as demonstrated by FISH and microcalorimetry. Osteoblast viability and differentiation were not negatively affected by the AMP. A cation concentration comparable to that physiologically occurring in blood had almost no negative effect on AMP activity and even with 10% serum bacterial growth was inhibited. Bacteria internalized into osteoblasts were reduced by the AMP. Taken together the results demonstrate a high antimicrobial activity of the AMP even against bacteria incorporated in a biofilm or internalized into cells without harming human osteoblasts.N. BormannA. KoliszakS. KasperL. SchoenK. HilpertR. VolkmerJ. KikhneyB. WildemannNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 7, Iss 1, Pp 1-14 (2017)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q N. Bormann A. Koliszak S. Kasper L. Schoen K. Hilpert R. Volkmer J. Kikhney B. Wildemann A short artificial antimicrobial peptide shows potential to prevent or treat bone infections
description	Abstract Infection of bone is a severe complication due to the variety of bacteria causing it, their resistance against classical antibiotics, the formation of a biofilm and the difficulty to eradicate it. Antimicrobial peptides (AMPs) are naturally occurring peptides and promising candidates for treatment of joint infections. This study aimed to analyze the effect of short artificial peptides derived from an optimized library regarding (1) antimicrobial effect on different bacterial species, (2) efficacy on biofilms, and (3) effect on osteoblast‑like cells. Culturing the AMP-modifications with Escherichia coli, Enterococcus faecalis, Pseudomonas aeruginosa, Staphylococcus aureus (including clinical isolates of MRSA and MSSA) and Staphylococcus epidermidis identified one candidate that was most effective against all bacteria. This AMP was also able to reduce biofilm as demonstrated by FISH and microcalorimetry. Osteoblast viability and differentiation were not negatively affected by the AMP. A cation concentration comparable to that physiologically occurring in blood had almost no negative effect on AMP activity and even with 10% serum bacterial growth was inhibited. Bacteria internalized into osteoblasts were reduced by the AMP. Taken together the results demonstrate a high antimicrobial activity of the AMP even against bacteria incorporated in a biofilm or internalized into cells without harming human osteoblasts.
format	article
author	N. Bormann A. Koliszak S. Kasper L. Schoen K. Hilpert R. Volkmer J. Kikhney B. Wildemann
author_facet	N. Bormann A. Koliszak S. Kasper L. Schoen K. Hilpert R. Volkmer J. Kikhney B. Wildemann
author_sort	N. Bormann
title	A short artificial antimicrobial peptide shows potential to prevent or treat bone infections
title_short	A short artificial antimicrobial peptide shows potential to prevent or treat bone infections
title_full	A short artificial antimicrobial peptide shows potential to prevent or treat bone infections
title_fullStr	A short artificial antimicrobial peptide shows potential to prevent or treat bone infections
title_full_unstemmed	A short artificial antimicrobial peptide shows potential to prevent or treat bone infections
title_sort	short artificial antimicrobial peptide shows potential to prevent or treat bone infections
publisher	Nature Portfolio
publishDate	2017
url	https://doaj.org/article/8cc49451c0b64c579e355aeb8941cf1a
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A short artificial antimicrobial peptide shows potential to prevent or treat bone infections

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