The complete genome of 2,6-dichlorobenzamide (BAM) degrader Aminobacter sp. MSH1 suggests a polyploid chromosome, phylogenetic reassignment, and functions of plasmids
Abstract Aminobacter sp. MSH1 (CIP 110285) can use the pesticide dichlobenil and its recalcitrant transformation product, 2,6-dichlorobenzamide (BAM), as sole source of carbon, nitrogen, and energy. The concentration of BAM in groundwater often exceeds the threshold limit for drinking water, requiri...
Guardado en:
| Autores principales: | , , , , , , , , , , |
|---|---|
| Formato: | article |
| Lenguaje: | EN |
| Publicado: |
Nature Portfolio
2021
|
| Materias: | |
| Acceso en línea: | https://doaj.org/article/8cc5a0b2f2544cdab167cb875ec552b5 |
| Etiquetas: |
Agregar Etiqueta
Sin Etiquetas, Sea el primero en etiquetar este registro!
|
| id |
oai:doaj.org-article:8cc5a0b2f2544cdab167cb875ec552b5 |
|---|---|
| record_format |
dspace |
| spelling |
oai:doaj.org-article:8cc5a0b2f2544cdab167cb875ec552b52021-12-02T15:14:55ZThe complete genome of 2,6-dichlorobenzamide (BAM) degrader Aminobacter sp. MSH1 suggests a polyploid chromosome, phylogenetic reassignment, and functions of plasmids10.1038/s41598-021-98184-52045-2322https://doaj.org/article/8cc5a0b2f2544cdab167cb875ec552b52021-09-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-98184-5https://doaj.org/toc/2045-2322Abstract Aminobacter sp. MSH1 (CIP 110285) can use the pesticide dichlobenil and its recalcitrant transformation product, 2,6-dichlorobenzamide (BAM), as sole source of carbon, nitrogen, and energy. The concentration of BAM in groundwater often exceeds the threshold limit for drinking water, requiring additional treatment in drinking water treatment plants or closure of the affected abstraction wells. Biological treatment with MSH1 is considered a potential sustainable alternative to remediate BAM-contamination in drinking water production. We present the complete genome of MSH1, which was determined independently in two institutes at Aarhus University and KU Leuven. Divergences were observed between the two genomes, i.e. one of them lacked four plasmids compared to the other. Besides the circular chromosome and the two previously described plasmids involved in BAM catabolism, pBAM1 and pBAM2, the genome of MSH1 contained two megaplasmids and three smaller plasmids. The MSH1 substrain from KU Leuven showed a reduced genome lacking a megaplasmid and three smaller plasmids and was designated substrain MK1, whereas the Aarhus variant with all plasmids was designated substrain DK1. A plasmid stability experiment indicate that substrain DK1 may have a polyploid chromosome when growing in R2B medium with more chromosomes than plasmids per cell. Finally, strain MSH1 is reassigned as Aminobacter niigataensis MSH1.Tue Kjærgaard NielsenBenjamin HoremansCédric LoodJeroen T’SyenVera van NoortRob LavigneLea Ellegaard-JensenOle HyllingJens AamandDirk SpringaelLars Hestbjerg HansenNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-12 (2021) |
| institution |
DOAJ |
| collection |
DOAJ |
| language |
EN |
| topic |
Medicine R Science Q |
| spellingShingle |
Medicine R Science Q Tue Kjærgaard Nielsen Benjamin Horemans Cédric Lood Jeroen T’Syen Vera van Noort Rob Lavigne Lea Ellegaard-Jensen Ole Hylling Jens Aamand Dirk Springael Lars Hestbjerg Hansen The complete genome of 2,6-dichlorobenzamide (BAM) degrader Aminobacter sp. MSH1 suggests a polyploid chromosome, phylogenetic reassignment, and functions of plasmids |
| description |
Abstract Aminobacter sp. MSH1 (CIP 110285) can use the pesticide dichlobenil and its recalcitrant transformation product, 2,6-dichlorobenzamide (BAM), as sole source of carbon, nitrogen, and energy. The concentration of BAM in groundwater often exceeds the threshold limit for drinking water, requiring additional treatment in drinking water treatment plants or closure of the affected abstraction wells. Biological treatment with MSH1 is considered a potential sustainable alternative to remediate BAM-contamination in drinking water production. We present the complete genome of MSH1, which was determined independently in two institutes at Aarhus University and KU Leuven. Divergences were observed between the two genomes, i.e. one of them lacked four plasmids compared to the other. Besides the circular chromosome and the two previously described plasmids involved in BAM catabolism, pBAM1 and pBAM2, the genome of MSH1 contained two megaplasmids and three smaller plasmids. The MSH1 substrain from KU Leuven showed a reduced genome lacking a megaplasmid and three smaller plasmids and was designated substrain MK1, whereas the Aarhus variant with all plasmids was designated substrain DK1. A plasmid stability experiment indicate that substrain DK1 may have a polyploid chromosome when growing in R2B medium with more chromosomes than plasmids per cell. Finally, strain MSH1 is reassigned as Aminobacter niigataensis MSH1. |
| format |
article |
| author |
Tue Kjærgaard Nielsen Benjamin Horemans Cédric Lood Jeroen T’Syen Vera van Noort Rob Lavigne Lea Ellegaard-Jensen Ole Hylling Jens Aamand Dirk Springael Lars Hestbjerg Hansen |
| author_facet |
Tue Kjærgaard Nielsen Benjamin Horemans Cédric Lood Jeroen T’Syen Vera van Noort Rob Lavigne Lea Ellegaard-Jensen Ole Hylling Jens Aamand Dirk Springael Lars Hestbjerg Hansen |
| author_sort |
Tue Kjærgaard Nielsen |
| title |
The complete genome of 2,6-dichlorobenzamide (BAM) degrader Aminobacter sp. MSH1 suggests a polyploid chromosome, phylogenetic reassignment, and functions of plasmids |
| title_short |
The complete genome of 2,6-dichlorobenzamide (BAM) degrader Aminobacter sp. MSH1 suggests a polyploid chromosome, phylogenetic reassignment, and functions of plasmids |
| title_full |
The complete genome of 2,6-dichlorobenzamide (BAM) degrader Aminobacter sp. MSH1 suggests a polyploid chromosome, phylogenetic reassignment, and functions of plasmids |
| title_fullStr |
The complete genome of 2,6-dichlorobenzamide (BAM) degrader Aminobacter sp. MSH1 suggests a polyploid chromosome, phylogenetic reassignment, and functions of plasmids |
| title_full_unstemmed |
The complete genome of 2,6-dichlorobenzamide (BAM) degrader Aminobacter sp. MSH1 suggests a polyploid chromosome, phylogenetic reassignment, and functions of plasmids |
| title_sort |
complete genome of 2,6-dichlorobenzamide (bam) degrader aminobacter sp. msh1 suggests a polyploid chromosome, phylogenetic reassignment, and functions of plasmids |
| publisher |
Nature Portfolio |
| publishDate |
2021 |
| url |
https://doaj.org/article/8cc5a0b2f2544cdab167cb875ec552b5 |
| work_keys_str_mv |
AT tuekjærgaardnielsen thecompletegenomeof26dichlorobenzamidebamdegraderaminobacterspmsh1suggestsapolyploidchromosomephylogeneticreassignmentandfunctionsofplasmids AT benjaminhoremans thecompletegenomeof26dichlorobenzamidebamdegraderaminobacterspmsh1suggestsapolyploidchromosomephylogeneticreassignmentandfunctionsofplasmids AT cedriclood thecompletegenomeof26dichlorobenzamidebamdegraderaminobacterspmsh1suggestsapolyploidchromosomephylogeneticreassignmentandfunctionsofplasmids AT jeroentsyen thecompletegenomeof26dichlorobenzamidebamdegraderaminobacterspmsh1suggestsapolyploidchromosomephylogeneticreassignmentandfunctionsofplasmids AT veravannoort thecompletegenomeof26dichlorobenzamidebamdegraderaminobacterspmsh1suggestsapolyploidchromosomephylogeneticreassignmentandfunctionsofplasmids AT roblavigne thecompletegenomeof26dichlorobenzamidebamdegraderaminobacterspmsh1suggestsapolyploidchromosomephylogeneticreassignmentandfunctionsofplasmids AT leaellegaardjensen thecompletegenomeof26dichlorobenzamidebamdegraderaminobacterspmsh1suggestsapolyploidchromosomephylogeneticreassignmentandfunctionsofplasmids AT olehylling thecompletegenomeof26dichlorobenzamidebamdegraderaminobacterspmsh1suggestsapolyploidchromosomephylogeneticreassignmentandfunctionsofplasmids AT jensaamand thecompletegenomeof26dichlorobenzamidebamdegraderaminobacterspmsh1suggestsapolyploidchromosomephylogeneticreassignmentandfunctionsofplasmids AT dirkspringael thecompletegenomeof26dichlorobenzamidebamdegraderaminobacterspmsh1suggestsapolyploidchromosomephylogeneticreassignmentandfunctionsofplasmids AT larshestbjerghansen thecompletegenomeof26dichlorobenzamidebamdegraderaminobacterspmsh1suggestsapolyploidchromosomephylogeneticreassignmentandfunctionsofplasmids AT tuekjærgaardnielsen completegenomeof26dichlorobenzamidebamdegraderaminobacterspmsh1suggestsapolyploidchromosomephylogeneticreassignmentandfunctionsofplasmids AT benjaminhoremans completegenomeof26dichlorobenzamidebamdegraderaminobacterspmsh1suggestsapolyploidchromosomephylogeneticreassignmentandfunctionsofplasmids AT cedriclood completegenomeof26dichlorobenzamidebamdegraderaminobacterspmsh1suggestsapolyploidchromosomephylogeneticreassignmentandfunctionsofplasmids AT jeroentsyen completegenomeof26dichlorobenzamidebamdegraderaminobacterspmsh1suggestsapolyploidchromosomephylogeneticreassignmentandfunctionsofplasmids AT veravannoort completegenomeof26dichlorobenzamidebamdegraderaminobacterspmsh1suggestsapolyploidchromosomephylogeneticreassignmentandfunctionsofplasmids AT roblavigne completegenomeof26dichlorobenzamidebamdegraderaminobacterspmsh1suggestsapolyploidchromosomephylogeneticreassignmentandfunctionsofplasmids AT leaellegaardjensen completegenomeof26dichlorobenzamidebamdegraderaminobacterspmsh1suggestsapolyploidchromosomephylogeneticreassignmentandfunctionsofplasmids AT olehylling completegenomeof26dichlorobenzamidebamdegraderaminobacterspmsh1suggestsapolyploidchromosomephylogeneticreassignmentandfunctionsofplasmids AT jensaamand completegenomeof26dichlorobenzamidebamdegraderaminobacterspmsh1suggestsapolyploidchromosomephylogeneticreassignmentandfunctionsofplasmids AT dirkspringael completegenomeof26dichlorobenzamidebamdegraderaminobacterspmsh1suggestsapolyploidchromosomephylogeneticreassignmentandfunctionsofplasmids AT larshestbjerghansen completegenomeof26dichlorobenzamidebamdegraderaminobacterspmsh1suggestsapolyploidchromosomephylogeneticreassignmentandfunctionsofplasmids |
| _version_ |
1718387581098393600 |