Combining LOPIT with differential ultracentrifugation for high-resolution spatial proteomics

Spatial proteomics allows studying cellular protein localisations at system-wide scale. Here, the authors show that combining the previously developed hyperLOPIT method with differential centrifugation yields protein localisation maps at suborganellar resolution while reducing analysis time and inpu...

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Autores principales:	Aikaterini Geladaki, Nina Kočevar Britovšek, Lisa M. Breckels, Tom S. Smith, Owen L. Vennard, Claire M. Mulvey, Oliver M. Crook, Laurent Gatto, Kathryn S. Lilley
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2019
Materias:	Science Q
Acceso en línea:	https://doaj.org/article/8cca2d3ddf7e46f093bba2ea911ecc37
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spelling	oai:doaj.org-article:8cca2d3ddf7e46f093bba2ea911ecc372021-12-02T16:57:24ZCombining LOPIT with differential ultracentrifugation for high-resolution spatial proteomics10.1038/s41467-018-08191-w2041-1723https://doaj.org/article/8cca2d3ddf7e46f093bba2ea911ecc372019-01-01T00:00:00Zhttps://doi.org/10.1038/s41467-018-08191-whttps://doaj.org/toc/2041-1723Spatial proteomics allows studying cellular protein localisations at system-wide scale. Here, the authors show that combining the previously developed hyperLOPIT method with differential centrifugation yields protein localisation maps at suborganellar resolution while reducing analysis time and input material.Aikaterini GeladakiNina Kočevar BritovšekLisa M. BreckelsTom S. SmithOwen L. VennardClaire M. MulveyOliver M. CrookLaurent GattoKathryn S. LilleyNature PortfolioarticleScienceQENNature Communications, Vol 10, Iss 1, Pp 1-15 (2019)
institution	DOAJ
collection	DOAJ
language	EN
topic	Science Q
spellingShingle	Science Q Aikaterini Geladaki Nina Kočevar Britovšek Lisa M. Breckels Tom S. Smith Owen L. Vennard Claire M. Mulvey Oliver M. Crook Laurent Gatto Kathryn S. Lilley Combining LOPIT with differential ultracentrifugation for high-resolution spatial proteomics
description	Spatial proteomics allows studying cellular protein localisations at system-wide scale. Here, the authors show that combining the previously developed hyperLOPIT method with differential centrifugation yields protein localisation maps at suborganellar resolution while reducing analysis time and input material.
format	article
author	Aikaterini Geladaki Nina Kočevar Britovšek Lisa M. Breckels Tom S. Smith Owen L. Vennard Claire M. Mulvey Oliver M. Crook Laurent Gatto Kathryn S. Lilley
author_facet	Aikaterini Geladaki Nina Kočevar Britovšek Lisa M. Breckels Tom S. Smith Owen L. Vennard Claire M. Mulvey Oliver M. Crook Laurent Gatto Kathryn S. Lilley
author_sort	Aikaterini Geladaki
title	Combining LOPIT with differential ultracentrifugation for high-resolution spatial proteomics
title_short	Combining LOPIT with differential ultracentrifugation for high-resolution spatial proteomics
title_full	Combining LOPIT with differential ultracentrifugation for high-resolution spatial proteomics
title_fullStr	Combining LOPIT with differential ultracentrifugation for high-resolution spatial proteomics
title_full_unstemmed	Combining LOPIT with differential ultracentrifugation for high-resolution spatial proteomics
title_sort	combining lopit with differential ultracentrifugation for high-resolution spatial proteomics
publisher	Nature Portfolio
publishDate	2019
url	https://doaj.org/article/8cca2d3ddf7e46f093bba2ea911ecc37
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Combining LOPIT with differential ultracentrifugation for high-resolution spatial proteomics

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