Combining LOPIT with differential ultracentrifugation for high-resolution spatial proteomics
Spatial proteomics allows studying cellular protein localisations at system-wide scale. Here, the authors show that combining the previously developed hyperLOPIT method with differential centrifugation yields protein localisation maps at suborganellar resolution while reducing analysis time and inpu...
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Autores principales: | Aikaterini Geladaki, Nina Kočevar Britovšek, Lisa M. Breckels, Tom S. Smith, Owen L. Vennard, Claire M. Mulvey, Oliver M. Crook, Laurent Gatto, Kathryn S. Lilley |
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Formato: | article |
Lenguaje: | EN |
Publicado: |
Nature Portfolio
2019
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Materias: | |
Acceso en línea: | https://doaj.org/article/8cca2d3ddf7e46f093bba2ea911ecc37 |
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