Mutagen-induced phytotoxicity in maize seed germination is dependent on ROS scavenging capacity

Abstract Ethidium bromide (EB) and acridine orange (AO) bind to nucleic acids and are thus considered as potential mutagens. In this study, the effects of EB and AO on the germination behaviours of white, yellow, red, and purple maize seeds were investigated. The results indicate that low concentrat...

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Autores principales:	Yifei Zhang, Haojie Shi, Benliang Deng
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2018
Materias:	Maize Seeds Germination Dimethylthiourea (DMTU) Diphenylene Iodonium (DPI) Yellow Seeds Medicine R Science Q
Acceso en línea:	https://doaj.org/article/8cca342150e2437dbc7091b412adb598
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spelling	oai:doaj.org-article:8cca342150e2437dbc7091b412adb5982021-12-02T15:08:10ZMutagen-induced phytotoxicity in maize seed germination is dependent on ROS scavenging capacity10.1038/s41598-018-32271-y2045-2322https://doaj.org/article/8cca342150e2437dbc7091b412adb5982018-09-01T00:00:00Zhttps://doi.org/10.1038/s41598-018-32271-yhttps://doaj.org/toc/2045-2322Abstract Ethidium bromide (EB) and acridine orange (AO) bind to nucleic acids and are thus considered as potential mutagens. In this study, the effects of EB and AO on the germination behaviours of white, yellow, red, and purple maize seeds were investigated. The results indicate that low concentrations of EB (50 μg mL−1) and AO (500 μg mL−1) promote germination, particularly for the white and yellow seeds. However, high concentrations of EB (0.5 mg mL−1) and AO (5 mg mL−1) significantly inhibit germination, with the level of inhibition decreasing in the following order: white > yellow > red > purple. In addition, EB and AO induce H2O2 production in a concentration-dependent manner. The effects of these mutagens on seed germination were partly reversed by dimethyl thiourea, a scavenger of reactive oxygen species (ROS), and diphenylene iodonium (DPI), an inhibitor of NADPH oxidase, while the effects were enhanced by treatment with H2O2 and 3-amino-1,2,4-triazole, a specific inhibitor of catalase. In addition, AO and EB profoundly increased NADPH oxidase activities in germinating seeds. The treatment of seeds with EB and AO did not affect the growth or drought tolerance of the resultant seedlings. The findings suggest that the mechanism of mutagen toxicity is related to the induction of ROS production.Yifei ZhangHaojie ShiBenliang DengNature PortfolioarticleMaize SeedsGerminationDimethylthiourea (DMTU)Diphenylene Iodonium (DPI)Yellow SeedsMedicineRScienceQENScientific Reports, Vol 8, Iss 1, Pp 1-10 (2018)
institution	DOAJ
collection	DOAJ
language	EN
topic	Maize Seeds Germination Dimethylthiourea (DMTU) Diphenylene Iodonium (DPI) Yellow Seeds Medicine R Science Q
spellingShingle	Maize Seeds Germination Dimethylthiourea (DMTU) Diphenylene Iodonium (DPI) Yellow Seeds Medicine R Science Q Yifei Zhang Haojie Shi Benliang Deng Mutagen-induced phytotoxicity in maize seed germination is dependent on ROS scavenging capacity
description	Abstract Ethidium bromide (EB) and acridine orange (AO) bind to nucleic acids and are thus considered as potential mutagens. In this study, the effects of EB and AO on the germination behaviours of white, yellow, red, and purple maize seeds were investigated. The results indicate that low concentrations of EB (50 μg mL−1) and AO (500 μg mL−1) promote germination, particularly for the white and yellow seeds. However, high concentrations of EB (0.5 mg mL−1) and AO (5 mg mL−1) significantly inhibit germination, with the level of inhibition decreasing in the following order: white > yellow > red > purple. In addition, EB and AO induce H2O2 production in a concentration-dependent manner. The effects of these mutagens on seed germination were partly reversed by dimethyl thiourea, a scavenger of reactive oxygen species (ROS), and diphenylene iodonium (DPI), an inhibitor of NADPH oxidase, while the effects were enhanced by treatment with H2O2 and 3-amino-1,2,4-triazole, a specific inhibitor of catalase. In addition, AO and EB profoundly increased NADPH oxidase activities in germinating seeds. The treatment of seeds with EB and AO did not affect the growth or drought tolerance of the resultant seedlings. The findings suggest that the mechanism of mutagen toxicity is related to the induction of ROS production.
format	article
author	Yifei Zhang Haojie Shi Benliang Deng
author_facet	Yifei Zhang Haojie Shi Benliang Deng
author_sort	Yifei Zhang
title	Mutagen-induced phytotoxicity in maize seed germination is dependent on ROS scavenging capacity
title_short	Mutagen-induced phytotoxicity in maize seed germination is dependent on ROS scavenging capacity
title_full	Mutagen-induced phytotoxicity in maize seed germination is dependent on ROS scavenging capacity
title_fullStr	Mutagen-induced phytotoxicity in maize seed germination is dependent on ROS scavenging capacity
title_full_unstemmed	Mutagen-induced phytotoxicity in maize seed germination is dependent on ROS scavenging capacity
title_sort	mutagen-induced phytotoxicity in maize seed germination is dependent on ros scavenging capacity
publisher	Nature Portfolio
publishDate	2018
url	https://doaj.org/article/8cca342150e2437dbc7091b412adb598
work_keys_str_mv	AT yifeizhang mutageninducedphytotoxicityinmaizeseedgerminationisdependentonrosscavengingcapacity AT haojieshi mutageninducedphytotoxicityinmaizeseedgerminationisdependentonrosscavengingcapacity AT benliangdeng mutageninducedphytotoxicityinmaizeseedgerminationisdependentonrosscavengingcapacity
_version_	1718388262388629504

Mutagen-induced phytotoxicity in maize seed germination is dependent on ROS scavenging capacity

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