Sensitivity of <i>Streptococcus</i> <i>viridans</i> to antibacterial agents in HIV-positive patients coupled to respiratory diseases

The streptococcus group consists of multiple species of globular Gram-positive facultative anaerobic bacteria, the classification of which is based on assessing their capacity to erythrocyte hemolysis. The viridans group streptococci produce a green coloration being a part of normal microflora in th...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	L. V. Puzyreva, A. V. Mordyk, L. A. Rodkina, I. V. Zhitina, A. V. Timofeeva
Formato:	article
Lenguaje:	RU
Publicado:	Sankt-Peterburg : NIIÈM imeni Pastera 2021
Materias:	sputum microflora streptococci viridans hiv infection pneumonia antibiotic sensitivity minimal inhibitory concentration Infectious and parasitic diseases RC109-216
Acceso en línea:	https://doaj.org/article/8cca52292bdf46798cb964a133e45135
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:8cca52292bdf46798cb964a133e45135
record_format	dspace
spelling	oai:doaj.org-article:8cca52292bdf46798cb964a133e451352021-11-22T07:09:54ZSensitivity of <i>Streptococcus</i> <i>viridans</i> to antibacterial agents in HIV-positive patients coupled to respiratory diseases2220-76192313-739810.15789/2220-7619-SOS-1241https://doaj.org/article/8cca52292bdf46798cb964a133e451352021-03-01T00:00:00Zhttps://www.iimmun.ru/iimm/article/view/1241https://doaj.org/toc/2220-7619https://doaj.org/toc/2313-7398The streptococcus group consists of multiple species of globular Gram-positive facultative anaerobic bacteria, the classification of which is based on assessing their capacity to erythrocyte hemolysis. The viridans group streptococci produce a green coloration being a part of normal microflora in the oral cavity. However, in case of developing immunodeficiency states, oncology, and neutropenia, this pathogen can cause diseases such as bacteremia, sepsis, endocarditis, as well as pneumonia in some cases. Respiratory diseases commonly require medical assistance in HIV-infected patient cohort. The role of commensal S. viridans in development of bacterial diseases in HIV-infected patients remains poorly examined. A potential association between S. viridans and other bacterial communities, including pathogenic microorganisms is obscure. In addition, it might be worth evaluating sensitivity of S. viridans to antibacterial agents in regions with high HIV infection incidence. The aim of the study was to evaluate sensitivity to antibiotics in sputum viridans group streptococci collected from HIV-infected patients with symptomatic pneumonia. Materials and methods. The data on sputum microbiological study conducted within the 01.01.2012—01.01.2019 interval were collected from all HIV-infected patients with suspected pneumonia (n = 684) and analyzed at the Infectious Clinical Hospital No. 1 named after D.M. Dalmatov, among which S. viridans was detected in 193 patients (28.2%). Bacteriological examination of patient samples was conducted to isolate pure bacterial culture by using standard methods. Next, S. viridans was assessed for sensitivity to antibacterial agents (n = 78) according to the 2018 clinical recommendations. Initially, antibiotic resistance was assessed by disk-diffusion method as a routine approach broadly used in clinical laboratory. After that, the minimal inhibitory concentration was measured by using AutoScan 4 Microscan analyzer (bacteriological semi-automatic analyzer, Beckman Coulter, USA) to identify and determine antibacterial sensitivity. Results. A disk-diffusion method allowed to find in sputum samples streptococci resistant to penicillin in 28.2% cases, ampicillin — in 42.3% and cefazolin — in 43.6%. While assessing the minimal inhibitory concentration for antibacterial agents, similar data were obtained. Good sensitivity was found for cefotaxime, ceftriaxone, cefepime, fluoroquinolones and vancomycin.L. V. PuzyrevaA. V. MordykL. A. RodkinaI. V. ZhitinaA. V. TimofeevaSankt-Peterburg : NIIÈM imeni Pasteraarticlesputum microflorastreptococci viridanshiv infectionpneumoniaantibiotic sensitivityminimal inhibitory concentrationInfectious and parasitic diseasesRC109-216RUInfekciâ i Immunitet, Vol 11, Iss 2, Pp 371-376 (2021)
institution	DOAJ
collection	DOAJ
language	RU
topic	sputum microflora streptococci viridans hiv infection pneumonia antibiotic sensitivity minimal inhibitory concentration Infectious and parasitic diseases RC109-216
spellingShingle	sputum microflora streptococci viridans hiv infection pneumonia antibiotic sensitivity minimal inhibitory concentration Infectious and parasitic diseases RC109-216 L. V. Puzyreva A. V. Mordyk L. A. Rodkina I. V. Zhitina A. V. Timofeeva Sensitivity of <i>Streptococcus</i> <i>viridans</i> to antibacterial agents in HIV-positive patients coupled to respiratory diseases
description	The streptococcus group consists of multiple species of globular Gram-positive facultative anaerobic bacteria, the classification of which is based on assessing their capacity to erythrocyte hemolysis. The viridans group streptococci produce a green coloration being a part of normal microflora in the oral cavity. However, in case of developing immunodeficiency states, oncology, and neutropenia, this pathogen can cause diseases such as bacteremia, sepsis, endocarditis, as well as pneumonia in some cases. Respiratory diseases commonly require medical assistance in HIV-infected patient cohort. The role of commensal S. viridans in development of bacterial diseases in HIV-infected patients remains poorly examined. A potential association between S. viridans and other bacterial communities, including pathogenic microorganisms is obscure. In addition, it might be worth evaluating sensitivity of S. viridans to antibacterial agents in regions with high HIV infection incidence. The aim of the study was to evaluate sensitivity to antibiotics in sputum viridans group streptococci collected from HIV-infected patients with symptomatic pneumonia. Materials and methods. The data on sputum microbiological study conducted within the 01.01.2012—01.01.2019 interval were collected from all HIV-infected patients with suspected pneumonia (n = 684) and analyzed at the Infectious Clinical Hospital No. 1 named after D.M. Dalmatov, among which S. viridans was detected in 193 patients (28.2%). Bacteriological examination of patient samples was conducted to isolate pure bacterial culture by using standard methods. Next, S. viridans was assessed for sensitivity to antibacterial agents (n = 78) according to the 2018 clinical recommendations. Initially, antibiotic resistance was assessed by disk-diffusion method as a routine approach broadly used in clinical laboratory. After that, the minimal inhibitory concentration was measured by using AutoScan 4 Microscan analyzer (bacteriological semi-automatic analyzer, Beckman Coulter, USA) to identify and determine antibacterial sensitivity. Results. A disk-diffusion method allowed to find in sputum samples streptococci resistant to penicillin in 28.2% cases, ampicillin — in 42.3% and cefazolin — in 43.6%. While assessing the minimal inhibitory concentration for antibacterial agents, similar data were obtained. Good sensitivity was found for cefotaxime, ceftriaxone, cefepime, fluoroquinolones and vancomycin.
format	article
author	L. V. Puzyreva A. V. Mordyk L. A. Rodkina I. V. Zhitina A. V. Timofeeva
author_facet	L. V. Puzyreva A. V. Mordyk L. A. Rodkina I. V. Zhitina A. V. Timofeeva
author_sort	L. V. Puzyreva
title	Sensitivity of <i>Streptococcus</i> <i>viridans</i> to antibacterial agents in HIV-positive patients coupled to respiratory diseases
title_short	Sensitivity of <i>Streptococcus</i> <i>viridans</i> to antibacterial agents in HIV-positive patients coupled to respiratory diseases
title_full	Sensitivity of <i>Streptococcus</i> <i>viridans</i> to antibacterial agents in HIV-positive patients coupled to respiratory diseases
title_fullStr	Sensitivity of <i>Streptococcus</i> <i>viridans</i> to antibacterial agents in HIV-positive patients coupled to respiratory diseases
title_full_unstemmed	Sensitivity of <i>Streptococcus</i> <i>viridans</i> to antibacterial agents in HIV-positive patients coupled to respiratory diseases
title_sort	sensitivity of <i>streptococcus</i> <i>viridans</i> to antibacterial agents in hiv-positive patients coupled to respiratory diseases
publisher	Sankt-Peterburg : NIIÈM imeni Pastera
publishDate	2021
url	https://doaj.org/article/8cca52292bdf46798cb964a133e45135
work_keys_str_mv	AT lvpuzyreva sensitivityofistreptococcusiiviridansitoantibacterialagentsinhivpositivepatientscoupledtorespiratorydiseases AT avmordyk sensitivityofistreptococcusiiviridansitoantibacterialagentsinhivpositivepatientscoupledtorespiratorydiseases AT larodkina sensitivityofistreptococcusiiviridansitoantibacterialagentsinhivpositivepatientscoupledtorespiratorydiseases AT ivzhitina sensitivityofistreptococcusiiviridansitoantibacterialagentsinhivpositivepatientscoupledtorespiratorydiseases AT avtimofeeva sensitivityofistreptococcusiiviridansitoantibacterialagentsinhivpositivepatientscoupledtorespiratorydiseases
_version_	1718417929711648768

Sensitivity of <i>Streptococcus</i> <i>viridans</i> to antibacterial agents in HIV-positive patients coupled to respiratory diseases

Ejemplares similares