Cytokinin Signaling in <named-content content-type="genus-species">Mycobacterium tuberculosis</named-content>

ABSTRACT It was recently reported that the human-exclusive pathogen Mycobacterium tuberculosis secretes cytokinins, which had only been known as plant hormones. While cytokinins are well-established, adenine-based signaling molecules in plants, they have never been shown to participate in signal tra...

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Autores principales:	Marie I. Samanovic, Hao-Chi Hsu, Marcus B. Jones, Victoria Jones, Michael R. McNeil, Samuel H. Becker, Ashley T. Jordan, Miroslav Strnad, Changcheng Xu, Mary Jackson, Huilin Li, K. Heran Darwin
Formato:	article
Lenguaje:	EN
Publicado:	American Society for Microbiology 2018
Materias:	Mycobacterium tuberculosis acid-fast staining cytokinin regulation signaling Microbiology QR1-502
Acceso en línea:	https://doaj.org/article/8cd03f758378472fbebf93b3106723bd
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spelling	oai:doaj.org-article:8cd03f758378472fbebf93b3106723bd2021-11-15T16:00:25ZCytokinin Signaling in <named-content content-type="genus-species">Mycobacterium tuberculosis</named-content>10.1128/mBio.00989-182150-7511https://doaj.org/article/8cd03f758378472fbebf93b3106723bd2018-07-01T00:00:00Zhttps://journals.asm.org/doi/10.1128/mBio.00989-18https://doaj.org/toc/2150-7511ABSTRACT It was recently reported that the human-exclusive pathogen Mycobacterium tuberculosis secretes cytokinins, which had only been known as plant hormones. While cytokinins are well-established, adenine-based signaling molecules in plants, they have never been shown to participate in signal transduction in other kingdoms of life. M. tuberculosis is not known to interact with plants. Therefore, we tested the hypothesis that cytokinins trigger transcriptional changes within this bacterial species. Here, we show cytokinins induced the strong expression of the M. tuberculosis gene Rv0077c. We found that Rv0077c expression is repressed by a TetR-like transcriptional repressor, Rv0078. Strikingly, cytokinin-induced expression of Rv0077c resulted in a loss of acid-fast staining of M. tuberculosis. While acid-fast staining is thought to be associated with changes in the bacterial cell envelope and virulence, Rv0077c-induced loss of acid-fastness did not affect antibiotic susceptibility or attenuate bacterial growth in mice, consistent with an unaltered mycolic acid profile of Rv0077c-expressing cells. Collectively, these findings show cytokinins signal transcriptional changes that can affect M. tuberculosis acid-fastness and that cytokinin signaling is no longer limited to the kingdom Plantae. IMPORTANCE Cytokinins have only previously been known as plant hormones. The discovery that they can be used as signaling molecules outside of plants broadens the repertoire of small molecules that can potentially affect gene expression in all domains of life.Marie I. SamanovicHao-Chi HsuMarcus B. JonesVictoria JonesMichael R. McNeilSamuel H. BeckerAshley T. JordanMiroslav StrnadChangcheng XuMary JacksonHuilin LiK. Heran DarwinAmerican Society for MicrobiologyarticleMycobacterium tuberculosisacid-fast stainingcytokininregulationsignalingMicrobiologyQR1-502ENmBio, Vol 9, Iss 3 (2018)
institution	DOAJ
collection	DOAJ
language	EN
topic	Mycobacterium tuberculosis acid-fast staining cytokinin regulation signaling Microbiology QR1-502
spellingShingle	Mycobacterium tuberculosis acid-fast staining cytokinin regulation signaling Microbiology QR1-502 Marie I. Samanovic Hao-Chi Hsu Marcus B. Jones Victoria Jones Michael R. McNeil Samuel H. Becker Ashley T. Jordan Miroslav Strnad Changcheng Xu Mary Jackson Huilin Li K. Heran Darwin Cytokinin Signaling in <named-content content-type="genus-species">Mycobacterium tuberculosis</named-content>
description	ABSTRACT It was recently reported that the human-exclusive pathogen Mycobacterium tuberculosis secretes cytokinins, which had only been known as plant hormones. While cytokinins are well-established, adenine-based signaling molecules in plants, they have never been shown to participate in signal transduction in other kingdoms of life. M. tuberculosis is not known to interact with plants. Therefore, we tested the hypothesis that cytokinins trigger transcriptional changes within this bacterial species. Here, we show cytokinins induced the strong expression of the M. tuberculosis gene Rv0077c. We found that Rv0077c expression is repressed by a TetR-like transcriptional repressor, Rv0078. Strikingly, cytokinin-induced expression of Rv0077c resulted in a loss of acid-fast staining of M. tuberculosis. While acid-fast staining is thought to be associated with changes in the bacterial cell envelope and virulence, Rv0077c-induced loss of acid-fastness did not affect antibiotic susceptibility or attenuate bacterial growth in mice, consistent with an unaltered mycolic acid profile of Rv0077c-expressing cells. Collectively, these findings show cytokinins signal transcriptional changes that can affect M. tuberculosis acid-fastness and that cytokinin signaling is no longer limited to the kingdom Plantae. IMPORTANCE Cytokinins have only previously been known as plant hormones. The discovery that they can be used as signaling molecules outside of plants broadens the repertoire of small molecules that can potentially affect gene expression in all domains of life.
format	article
author	Marie I. Samanovic Hao-Chi Hsu Marcus B. Jones Victoria Jones Michael R. McNeil Samuel H. Becker Ashley T. Jordan Miroslav Strnad Changcheng Xu Mary Jackson Huilin Li K. Heran Darwin
author_facet	Marie I. Samanovic Hao-Chi Hsu Marcus B. Jones Victoria Jones Michael R. McNeil Samuel H. Becker Ashley T. Jordan Miroslav Strnad Changcheng Xu Mary Jackson Huilin Li K. Heran Darwin
author_sort	Marie I. Samanovic
title	Cytokinin Signaling in <named-content content-type="genus-species">Mycobacterium tuberculosis</named-content>
title_short	Cytokinin Signaling in <named-content content-type="genus-species">Mycobacterium tuberculosis</named-content>
title_full	Cytokinin Signaling in <named-content content-type="genus-species">Mycobacterium tuberculosis</named-content>
title_fullStr	Cytokinin Signaling in <named-content content-type="genus-species">Mycobacterium tuberculosis</named-content>
title_full_unstemmed	Cytokinin Signaling in <named-content content-type="genus-species">Mycobacterium tuberculosis</named-content>
title_sort	cytokinin signaling in <named-content content-type="genus-species">mycobacterium tuberculosis</named-content>
publisher	American Society for Microbiology
publishDate	2018
url	https://doaj.org/article/8cd03f758378472fbebf93b3106723bd
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Cytokinin Signaling in <named-content content-type="genus-species">Mycobacterium tuberculosis</named-content>

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