Genetic diversity of local corn (Zea mays) cultivars from South Amarasi, Kupang District, Indonesia by Inter Simple Sequence Repeats marker

Abstract. Uslan, Jannah N. 2020. Genetic diversity of local corn (Zea mays) cultivars from South Amarasi, Kupang District, Indonesia by Inter Simple Sequence Repeats marker. Biodiversitas 21: 1208-1214. Corn (Zea mays L.) is one of the most important food crops in Indonesia. However, the studies des...

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Autores principales: Uslan Uslan, NUR JANNAH
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Publicado: MBI & UNS Solo 2020
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spelling oai:doaj.org-article:8cd20cb815604ba4af701be85c9311d82021-11-22T00:28:56ZGenetic diversity of local corn (Zea mays) cultivars from South Amarasi, Kupang District, Indonesia by Inter Simple Sequence Repeats marker1412-033X2085-472210.13057/biodiv/d210348https://doaj.org/article/8cd20cb815604ba4af701be85c9311d82020-02-01T00:00:00Zhttps://smujo.id/biodiv/article/view/5095https://doaj.org/toc/1412-033Xhttps://doaj.org/toc/2085-4722Abstract. Uslan, Jannah N. 2020. Genetic diversity of local corn (Zea mays) cultivars from South Amarasi, Kupang District, Indonesia by Inter Simple Sequence Repeats marker. Biodiversitas 21: 1208-1214. Corn (Zea mays L.) is one of the most important food crops in Indonesia. However, the studies described their genetic variation is relatively poor. Therefore, the aim of this study was to analyze the genetic diversity of local corn cultivars from South Amarasi, Kupang District, East Nusa Tenggara (NTT), Indonesia using ISSR markers. The sampling was conducted in 4 different sites in Sub-district of Amarasi, Kupang District. A total of 11 corn cultivars from Sub-district of South Amarasi was collected. DNA isolation was performed by using CTAB Method. Clustering analysis was conducted on MSVP 3.2 software. It was shown that all ISSR-primers used (UBC 811, UBC 814 and UBC 824) were successfully produced polymorphic bands and represents the high genetic diversity of the local corn cultivars. The genetic distance index indicated that several corn cultivars from two different populations were geographically unclustered, although there are samples from several populations that have a low genetic distance. The genetic variation index also showed high genetic diversity among the populations. Further research on the exhaustive sample collection was needed to give an insight into the genetic diversity of local corn cultivars (Zea mays L.) from South Amarasi, Indonesia. Please write implementation of this researchUslan UslanNUR JANNAHMBI & UNS Soloarticleissr, genetic diversity, east nusa tenggara, zea maysBiology (General)QH301-705.5ENBiodiversitas, Vol 21, Iss 3 (2020)
institution DOAJ
collection DOAJ
language EN
topic issr, genetic diversity, east nusa tenggara, zea mays
Biology (General)
QH301-705.5
spellingShingle issr, genetic diversity, east nusa tenggara, zea mays
Biology (General)
QH301-705.5
Uslan Uslan
NUR JANNAH
Genetic diversity of local corn (Zea mays) cultivars from South Amarasi, Kupang District, Indonesia by Inter Simple Sequence Repeats marker
description Abstract. Uslan, Jannah N. 2020. Genetic diversity of local corn (Zea mays) cultivars from South Amarasi, Kupang District, Indonesia by Inter Simple Sequence Repeats marker. Biodiversitas 21: 1208-1214. Corn (Zea mays L.) is one of the most important food crops in Indonesia. However, the studies described their genetic variation is relatively poor. Therefore, the aim of this study was to analyze the genetic diversity of local corn cultivars from South Amarasi, Kupang District, East Nusa Tenggara (NTT), Indonesia using ISSR markers. The sampling was conducted in 4 different sites in Sub-district of Amarasi, Kupang District. A total of 11 corn cultivars from Sub-district of South Amarasi was collected. DNA isolation was performed by using CTAB Method. Clustering analysis was conducted on MSVP 3.2 software. It was shown that all ISSR-primers used (UBC 811, UBC 814 and UBC 824) were successfully produced polymorphic bands and represents the high genetic diversity of the local corn cultivars. The genetic distance index indicated that several corn cultivars from two different populations were geographically unclustered, although there are samples from several populations that have a low genetic distance. The genetic variation index also showed high genetic diversity among the populations. Further research on the exhaustive sample collection was needed to give an insight into the genetic diversity of local corn cultivars (Zea mays L.) from South Amarasi, Indonesia. Please write implementation of this research
format article
author Uslan Uslan
NUR JANNAH
author_facet Uslan Uslan
NUR JANNAH
author_sort Uslan Uslan
title Genetic diversity of local corn (Zea mays) cultivars from South Amarasi, Kupang District, Indonesia by Inter Simple Sequence Repeats marker
title_short Genetic diversity of local corn (Zea mays) cultivars from South Amarasi, Kupang District, Indonesia by Inter Simple Sequence Repeats marker
title_full Genetic diversity of local corn (Zea mays) cultivars from South Amarasi, Kupang District, Indonesia by Inter Simple Sequence Repeats marker
title_fullStr Genetic diversity of local corn (Zea mays) cultivars from South Amarasi, Kupang District, Indonesia by Inter Simple Sequence Repeats marker
title_full_unstemmed Genetic diversity of local corn (Zea mays) cultivars from South Amarasi, Kupang District, Indonesia by Inter Simple Sequence Repeats marker
title_sort genetic diversity of local corn (zea mays) cultivars from south amarasi, kupang district, indonesia by inter simple sequence repeats marker
publisher MBI & UNS Solo
publishDate 2020
url https://doaj.org/article/8cd20cb815604ba4af701be85c9311d8
work_keys_str_mv AT uslanuslan geneticdiversityoflocalcornzeamayscultivarsfromsouthamarasikupangdistrictindonesiabyintersimplesequencerepeatsmarker
AT nurjannah geneticdiversityoflocalcornzeamayscultivarsfromsouthamarasikupangdistrictindonesiabyintersimplesequencerepeatsmarker
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