Peptide-mediated targeting of liposomes to TrkB receptor-expressing cells

Sanjeev Ranjan,1 Rohit Sood,1 Jozsef Dudas,2 Rudolf Glueckert,2 Anneliese Schrott-Fischer,2 Soumen Roy,2 Ilmari Pyykk&ouml;,3 Paavo KJ Kinnunen11Helsinki Biophysics and Biomembrane Group, Department of Biomedical Engineering and Computational Science, Aalto University, Espoo, Finland; 2Depar...

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Autores principales:	Ranjan S, Sood R, Dudas J, Glueckert R, Schrott-Fischer A, Roy S, Pyykk&ouml; I, Kinnunen PK
Formato:	article
Lenguaje:	EN
Publicado:	Dove Medical Press 2012
Materias:	Medicine (General) R5-920
Acceso en línea:	https://doaj.org/article/8cdd928c56164124acae707231bf319c
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spelling	oai:doaj.org-article:8cdd928c56164124acae707231bf319c2021-12-02T11:07:27ZPeptide-mediated targeting of liposomes to TrkB receptor-expressing cells1176-91141178-2013https://doaj.org/article/8cdd928c56164124acae707231bf319c2012-07-01T00:00:00Zhttp://www.dovepress.com/peptide-mediated-targeting-of-liposomes-to-trkb-receptor-expressing-ce-a10324https://doaj.org/toc/1176-9114https://doaj.org/toc/1178-2013Sanjeev Ranjan,1 Rohit Sood,1 Jozsef Dudas,2 Rudolf Glueckert,2 Anneliese Schrott-Fischer,2 Soumen Roy,2 Ilmari Pyykk&ouml;,3 Paavo KJ Kinnunen11Helsinki Biophysics and Biomembrane Group, Department of Biomedical Engineering and Computational Science, Aalto University, Espoo, Finland; 2Department of Otorhinolaryngology, Innsbruck Medical University, Innsbruck, Austria; 3Department of Otolaryngology, University of Tampere Medical School, Tampere, FinlandBackground: The neurotrophic receptor tyrosine kinase B (TrkB) has diverse signaling roles in neurons and tumor cells. Accordingly, its suppressive targeting is of interest in neuroblastoma and other tumors, whereas its role in improving survival is focused in neurons. Here we describe targeting of TrkB-binding peptide-conjugated liposomes (PCL) to the TrkB-expressing mouse macrophage-like cell line RAW264, and to all-trans-retinoic acid-treated neuron-like TrkB+ SH-SY5Y human neuroblastoma cells.Methods: Binding and internalization of PCL was monitored by flow cytometry and confocal fluorescence microscopy.Results: Internalization of TrkB-targeted PCL by RAW264 cells was enhanced and faster when compared with PCL having the corresponding scrambled peptide. Likewise, binding and augmented uptake were confirmed for TrkB+ SH-SY5Y cells, with targeted PCL appearing in the cytoplasm after 20 minutes of incubation.Conclusion: We demonstrate here the feasibility of targeting liposomes to TrkB-expressing cells by 18-mer peptides, promoting cellular uptake (at least partly into endosomes) via receptor-mediated pathways.Keywords: liposomes, targeting, tyrosine kinase B, peptideRanjan SSood RDudas JGlueckert RSchrott-Fischer ARoy SPyykk&ouml; IKinnunen PKDove Medical PressarticleMedicine (General)R5-920ENInternational Journal of Nanomedicine, Vol 2012, Iss default, Pp 3475-3485 (2012)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine (General) R5-920
spellingShingle	Medicine (General) R5-920 Ranjan S Sood R Dudas J Glueckert R Schrott-Fischer A Roy S Pyykk&ouml; I Kinnunen PK Peptide-mediated targeting of liposomes to TrkB receptor-expressing cells
description	Sanjeev Ranjan,1 Rohit Sood,1 Jozsef Dudas,2 Rudolf Glueckert,2 Anneliese Schrott-Fischer,2 Soumen Roy,2 Ilmari Pyykk&ouml;,3 Paavo KJ Kinnunen11Helsinki Biophysics and Biomembrane Group, Department of Biomedical Engineering and Computational Science, Aalto University, Espoo, Finland; 2Department of Otorhinolaryngology, Innsbruck Medical University, Innsbruck, Austria; 3Department of Otolaryngology, University of Tampere Medical School, Tampere, FinlandBackground: The neurotrophic receptor tyrosine kinase B (TrkB) has diverse signaling roles in neurons and tumor cells. Accordingly, its suppressive targeting is of interest in neuroblastoma and other tumors, whereas its role in improving survival is focused in neurons. Here we describe targeting of TrkB-binding peptide-conjugated liposomes (PCL) to the TrkB-expressing mouse macrophage-like cell line RAW264, and to all-trans-retinoic acid-treated neuron-like TrkB+ SH-SY5Y human neuroblastoma cells.Methods: Binding and internalization of PCL was monitored by flow cytometry and confocal fluorescence microscopy.Results: Internalization of TrkB-targeted PCL by RAW264 cells was enhanced and faster when compared with PCL having the corresponding scrambled peptide. Likewise, binding and augmented uptake were confirmed for TrkB+ SH-SY5Y cells, with targeted PCL appearing in the cytoplasm after 20 minutes of incubation.Conclusion: We demonstrate here the feasibility of targeting liposomes to TrkB-expressing cells by 18-mer peptides, promoting cellular uptake (at least partly into endosomes) via receptor-mediated pathways.Keywords: liposomes, targeting, tyrosine kinase B, peptide
format	article
author	Ranjan S Sood R Dudas J Glueckert R Schrott-Fischer A Roy S Pyykk&ouml; I Kinnunen PK
author_facet	Ranjan S Sood R Dudas J Glueckert R Schrott-Fischer A Roy S Pyykk&ouml; I Kinnunen PK
author_sort	Ranjan S
title	Peptide-mediated targeting of liposomes to TrkB receptor-expressing cells
title_short	Peptide-mediated targeting of liposomes to TrkB receptor-expressing cells
title_full	Peptide-mediated targeting of liposomes to TrkB receptor-expressing cells
title_fullStr	Peptide-mediated targeting of liposomes to TrkB receptor-expressing cells
title_full_unstemmed	Peptide-mediated targeting of liposomes to TrkB receptor-expressing cells
title_sort	peptide-mediated targeting of liposomes to trkb receptor-expressing cells
publisher	Dove Medical Press
publishDate	2012
url	https://doaj.org/article/8cdd928c56164124acae707231bf319c
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Peptide-mediated targeting of liposomes to TrkB receptor-expressing cells

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