ATF3 negatively regulates cellular antiviral signaling and autophagy in the absence of type I interferons

Abstract Stringent regulation of antiviral signaling and cellular autophagy is critical for the host response to virus infection. However, little is known how these cellular processes are regulated in the absence of type I interferon signaling. Here, we show that ATF3 is induced following Japanese e...

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Autores principales:	Vikas Sood, Kiran Bala Sharma, Vishal Gupta, Dhurjhoti Saha, Parashar Dhapola, Manish Sharma, Utsav Sen, Shigetaka Kitajima, Shantanu Chowdhury, Manjula Kalia, Sudhanshu Vrati
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2017
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/8ce5b5bafe084c7b89b6e768a4e4585a
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spelling	oai:doaj.org-article:8ce5b5bafe084c7b89b6e768a4e4585a2021-12-02T16:06:39ZATF3 negatively regulates cellular antiviral signaling and autophagy in the absence of type I interferons10.1038/s41598-017-08584-92045-2322https://doaj.org/article/8ce5b5bafe084c7b89b6e768a4e4585a2017-08-01T00:00:00Zhttps://doi.org/10.1038/s41598-017-08584-9https://doaj.org/toc/2045-2322Abstract Stringent regulation of antiviral signaling and cellular autophagy is critical for the host response to virus infection. However, little is known how these cellular processes are regulated in the absence of type I interferon signaling. Here, we show that ATF3 is induced following Japanese encephalitis virus (JEV) infection, and regulates cellular antiviral and autophagy pathways in the absence of type I interferons in mouse neuronal cells. We have identified new targets of ATF3 and show that it binds to the promoter regions of Stat1, Irf9, Isg15 and Atg5 thereby inhibiting cellular antiviral signaling and autophagy. Consistent with these observations, ATF3-depleted cells showed enhanced antiviral responses and induction of robust autophagy. Furthermore, we show that JEV replication was significantly reduced in ATF3-depleted cells. Our findings identify ATF3 as a negative regulator of antiviral signaling and cellular autophagy in mammalian cells, and demonstrate its important role in JEV life cycle.Vikas SoodKiran Bala SharmaVishal GuptaDhurjhoti SahaParashar DhapolaManish SharmaUtsav SenShigetaka KitajimaShantanu ChowdhuryManjula KaliaSudhanshu VratiNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 7, Iss 1, Pp 1-17 (2017)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Vikas Sood Kiran Bala Sharma Vishal Gupta Dhurjhoti Saha Parashar Dhapola Manish Sharma Utsav Sen Shigetaka Kitajima Shantanu Chowdhury Manjula Kalia Sudhanshu Vrati ATF3 negatively regulates cellular antiviral signaling and autophagy in the absence of type I interferons
description	Abstract Stringent regulation of antiviral signaling and cellular autophagy is critical for the host response to virus infection. However, little is known how these cellular processes are regulated in the absence of type I interferon signaling. Here, we show that ATF3 is induced following Japanese encephalitis virus (JEV) infection, and regulates cellular antiviral and autophagy pathways in the absence of type I interferons in mouse neuronal cells. We have identified new targets of ATF3 and show that it binds to the promoter regions of Stat1, Irf9, Isg15 and Atg5 thereby inhibiting cellular antiviral signaling and autophagy. Consistent with these observations, ATF3-depleted cells showed enhanced antiviral responses and induction of robust autophagy. Furthermore, we show that JEV replication was significantly reduced in ATF3-depleted cells. Our findings identify ATF3 as a negative regulator of antiviral signaling and cellular autophagy in mammalian cells, and demonstrate its important role in JEV life cycle.
format	article
author	Vikas Sood Kiran Bala Sharma Vishal Gupta Dhurjhoti Saha Parashar Dhapola Manish Sharma Utsav Sen Shigetaka Kitajima Shantanu Chowdhury Manjula Kalia Sudhanshu Vrati
author_facet	Vikas Sood Kiran Bala Sharma Vishal Gupta Dhurjhoti Saha Parashar Dhapola Manish Sharma Utsav Sen Shigetaka Kitajima Shantanu Chowdhury Manjula Kalia Sudhanshu Vrati
author_sort	Vikas Sood
title	ATF3 negatively regulates cellular antiviral signaling and autophagy in the absence of type I interferons
title_short	ATF3 negatively regulates cellular antiviral signaling and autophagy in the absence of type I interferons
title_full	ATF3 negatively regulates cellular antiviral signaling and autophagy in the absence of type I interferons
title_fullStr	ATF3 negatively regulates cellular antiviral signaling and autophagy in the absence of type I interferons
title_full_unstemmed	ATF3 negatively regulates cellular antiviral signaling and autophagy in the absence of type I interferons
title_sort	atf3 negatively regulates cellular antiviral signaling and autophagy in the absence of type i interferons
publisher	Nature Portfolio
publishDate	2017
url	https://doaj.org/article/8ce5b5bafe084c7b89b6e768a4e4585a
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ATF3 negatively regulates cellular antiviral signaling and autophagy in the absence of type I interferons

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