Occurrence of Staphylococcus piscifermentans among Apparently Healthy Ruminants and Their Handlers in Maiduguri, Borno State, Nigeria

This study was conducted to determine the occurrence and antimicrobial susceptibility of Staphylococcus piscifermentans nasal colonization among apparently healthy ruminants and their handlers in Maiduguri, Borno State, Nigeria. A total of six hundred and five (605) samples were collected asepticall...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Onyilokwu, S. A., Adamu, S. G., Lawan, F. A., Aye, A. M.
Formato:	article
Lenguaje:	EN
Publicado:	Egyptian Society for Animal Management 2021
Materias:	handlers occurrence piscifermentans ruminants susceptibility profiles Zoology QL1-991 Veterinary medicine SF600-1100 Animal biochemistry QP501-801
Acceso en línea:	https://dx.doi.org/10.21608/javs.2021.88440.1094 https://doaj.org/article/8cf06a6c9a954f25963950fea33feb83
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:8cf06a6c9a954f25963950fea33feb83
record_format	dspace
spelling	oai:doaj.org-article:8cf06a6c9a954f25963950fea33feb832021-12-02T19:13:43ZOccurrence of Staphylococcus piscifermentans among Apparently Healthy Ruminants and Their Handlers in Maiduguri, Borno State, Nigeriahttps://dx.doi.org/10.21608/javs.2021.88440.10941687-40722090-3308https://doaj.org/article/8cf06a6c9a954f25963950fea33feb832021-10-01T00:00:00Zhttps://javs.journals.ekb.eg/article_192695.htmlhttps://doaj.org/toc/1687-4072https://doaj.org/toc/2090-3308This study was conducted to determine the occurrence and antimicrobial susceptibility of Staphylococcus piscifermentans nasal colonization among apparently healthy ruminants and their handlers in Maiduguri, Borno State, Nigeria. A total of six hundred and five (605) samples were collected aseptically using sterile swab sticks (Oxoid, UK) using a simple random technique from the ruminants and their human handlers at the Maiduguri central abattoir and livestock market (Kasuwan shanu). One hundred and fifty-one (151) samples were collected from each species (cattle, sheep and goat), while 152 samples were collected from animal handlers. The samples were immediately subjected to standard bacteriological analysis. A total of 195 (32.2%) coagulase-negative Staphylococcus species (CoNS) were presumptively identified; out of the 195 identified CoNS, 9 (4.62%) was Staphylococcus piscifermentans nasal colonization. The results revealed that out of 9 isolates, S. piscifermentans was highly isolated 4(8.3%) from sheep, followed by cattle and human handlers 2 (3.8%) respectively, and the least was from goats 1 (2.3%). Meanwhile, all the 9 (4.62%) isolates were negative for both tube and slide coagulase test. However, they were positive for DNase, Ornithine Decarboxylase (ODC) test, betta – hemolysis and synergistic hemolysis. 4(44.4%) of the 9 isolates were positive for Staphylococcus Protein A (SPA) virulence gene, none 0(0%) for mecA virulence gene, and 5(55.6%) were positive for slime (Biofilm) production. The antibiotic susceptibility pattern of these isolates was found to be highly variable to all the used drugs. Therefore, it is concluded that Staphylococcus piscifermentans isolated from these ruminants and their handlers contain virulence genes that could be responsible for pathogenicity. There is a need for more studies using higher technologies in other parts of the country to reveal the thorough status of the disease in Nigeria.Onyilokwu, S. A.Adamu, S. G.Lawan, F. A.Aye, A. M.Egyptian Society for Animal Managementarticlehandlersoccurrencepiscifermentansruminantssusceptibility profilesZoologyQL1-991Veterinary medicineSF600-1100Animal biochemistryQP501-801ENJournal of Applied Veterinary Sciences, Vol 6, Iss 4, Pp 32-38 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	handlers occurrence piscifermentans ruminants susceptibility profiles Zoology QL1-991 Veterinary medicine SF600-1100 Animal biochemistry QP501-801
spellingShingle	handlers occurrence piscifermentans ruminants susceptibility profiles Zoology QL1-991 Veterinary medicine SF600-1100 Animal biochemistry QP501-801 Onyilokwu, S. A. Adamu, S. G. Lawan, F. A. Aye, A. M. Occurrence of Staphylococcus piscifermentans among Apparently Healthy Ruminants and Their Handlers in Maiduguri, Borno State, Nigeria
description	This study was conducted to determine the occurrence and antimicrobial susceptibility of Staphylococcus piscifermentans nasal colonization among apparently healthy ruminants and their handlers in Maiduguri, Borno State, Nigeria. A total of six hundred and five (605) samples were collected aseptically using sterile swab sticks (Oxoid, UK) using a simple random technique from the ruminants and their human handlers at the Maiduguri central abattoir and livestock market (Kasuwan shanu). One hundred and fifty-one (151) samples were collected from each species (cattle, sheep and goat), while 152 samples were collected from animal handlers. The samples were immediately subjected to standard bacteriological analysis. A total of 195 (32.2%) coagulase-negative Staphylococcus species (CoNS) were presumptively identified; out of the 195 identified CoNS, 9 (4.62%) was Staphylococcus piscifermentans nasal colonization. The results revealed that out of 9 isolates, S. piscifermentans was highly isolated 4(8.3%) from sheep, followed by cattle and human handlers 2 (3.8%) respectively, and the least was from goats 1 (2.3%). Meanwhile, all the 9 (4.62%) isolates were negative for both tube and slide coagulase test. However, they were positive for DNase, Ornithine Decarboxylase (ODC) test, betta – hemolysis and synergistic hemolysis. 4(44.4%) of the 9 isolates were positive for Staphylococcus Protein A (SPA) virulence gene, none 0(0%) for mecA virulence gene, and 5(55.6%) were positive for slime (Biofilm) production. The antibiotic susceptibility pattern of these isolates was found to be highly variable to all the used drugs. Therefore, it is concluded that Staphylococcus piscifermentans isolated from these ruminants and their handlers contain virulence genes that could be responsible for pathogenicity. There is a need for more studies using higher technologies in other parts of the country to reveal the thorough status of the disease in Nigeria.
format	article
author	Onyilokwu, S. A. Adamu, S. G. Lawan, F. A. Aye, A. M.
author_facet	Onyilokwu, S. A. Adamu, S. G. Lawan, F. A. Aye, A. M.
author_sort	Onyilokwu, S. A.
title	Occurrence of Staphylococcus piscifermentans among Apparently Healthy Ruminants and Their Handlers in Maiduguri, Borno State, Nigeria
title_short	Occurrence of Staphylococcus piscifermentans among Apparently Healthy Ruminants and Their Handlers in Maiduguri, Borno State, Nigeria
title_full	Occurrence of Staphylococcus piscifermentans among Apparently Healthy Ruminants and Their Handlers in Maiduguri, Borno State, Nigeria
title_fullStr	Occurrence of Staphylococcus piscifermentans among Apparently Healthy Ruminants and Their Handlers in Maiduguri, Borno State, Nigeria
title_full_unstemmed	Occurrence of Staphylococcus piscifermentans among Apparently Healthy Ruminants and Their Handlers in Maiduguri, Borno State, Nigeria
title_sort	occurrence of staphylococcus piscifermentans among apparently healthy ruminants and their handlers in maiduguri, borno state, nigeria
publisher	Egyptian Society for Animal Management
publishDate	2021
url	https://dx.doi.org/10.21608/javs.2021.88440.1094 https://doaj.org/article/8cf06a6c9a954f25963950fea33feb83
work_keys_str_mv	AT onyilokwusa occurrenceofstaphylococcuspiscifermentansamongapparentlyhealthyruminantsandtheirhandlersinmaiduguribornostatenigeria AT adamusg occurrenceofstaphylococcuspiscifermentansamongapparentlyhealthyruminantsandtheirhandlersinmaiduguribornostatenigeria AT lawanfa occurrenceofstaphylococcuspiscifermentansamongapparentlyhealthyruminantsandtheirhandlersinmaiduguribornostatenigeria AT ayeam occurrenceofstaphylococcuspiscifermentansamongapparentlyhealthyruminantsandtheirhandlersinmaiduguribornostatenigeria
_version_	1718377023452217344

Occurrence of Staphylococcus piscifermentans among Apparently Healthy Ruminants and Their Handlers in Maiduguri, Borno State, Nigeria

Ejemplares similares