Cytomegalovirus microRNAs facilitate persistent virus infection in salivary glands.

Micro (mi)RNAs are small non-coding RNAs that regulate the expression of their targets' messenger RNAs through both translational inhibition and regulation of target RNA stability. Recently, a number of viruses, particularly of the herpesvirus family, have been shown to express their own miRNAs...

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Autores principales:	Lars Dölken, Astrid Krmpotic, Sheila Kothe, Lee Tuddenham, Mélanie Tanguy, Lisa Marcinowski, Zsolt Ruzsics, Naama Elefant, Yael Altuvia, Hanah Margalit, Ulrich H Koszinowski, Stipan Jonjic, Sébastien Pfeffer
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2010
Materias:	Immunologic diseases. Allergy RC581-607 Biology (General) QH301-705.5
Acceso en línea:	https://doaj.org/article/8cf511385a6746a9a38533828dd0fe01
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spelling	oai:doaj.org-article:8cf511385a6746a9a38533828dd0fe012021-11-18T06:03:49ZCytomegalovirus microRNAs facilitate persistent virus infection in salivary glands.1553-73661553-737410.1371/journal.ppat.1001150https://doaj.org/article/8cf511385a6746a9a38533828dd0fe012010-10-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/20976200/?tool=EBIhttps://doaj.org/toc/1553-7366https://doaj.org/toc/1553-7374Micro (mi)RNAs are small non-coding RNAs that regulate the expression of their targets' messenger RNAs through both translational inhibition and regulation of target RNA stability. Recently, a number of viruses, particularly of the herpesvirus family, have been shown to express their own miRNAs to control both viral and cellular transcripts. Although some targets of viral miRNAs are known, their function in a physiologically relevant infection remains to be elucidated. As such, no in vivo phenotype of a viral miRNA knock-out mutant has been described so far. Here, we report on the first functional phenotype of a miRNA knock-out virus in vivo. During subacute infection of a mutant mouse cytomegalovirus lacking two viral miRNAs, virus production is selectively reduced in salivary glands, an organ essential for virus persistence and horizontal transmission. This phenotype depends on several parameters including viral load and mouse genetic background, and is abolished by combined but not single depletion of natural killer (NK) and CD4+ T cells. Together, our results point towards a miRNA-based immunoevasion mechanism important for long-term virus persistence.Lars DölkenAstrid KrmpoticSheila KotheLee TuddenhamMélanie TanguyLisa MarcinowskiZsolt RuzsicsNaama ElefantYael AltuviaHanah MargalitUlrich H KoszinowskiStipan JonjicSébastien PfefferPublic Library of Science (PLoS)articleImmunologic diseases. AllergyRC581-607Biology (General)QH301-705.5ENPLoS Pathogens, Vol 6, Iss 10, p e1001150 (2010)
institution	DOAJ
collection	DOAJ
language	EN
topic	Immunologic diseases. Allergy RC581-607 Biology (General) QH301-705.5
spellingShingle	Immunologic diseases. Allergy RC581-607 Biology (General) QH301-705.5 Lars Dölken Astrid Krmpotic Sheila Kothe Lee Tuddenham Mélanie Tanguy Lisa Marcinowski Zsolt Ruzsics Naama Elefant Yael Altuvia Hanah Margalit Ulrich H Koszinowski Stipan Jonjic Sébastien Pfeffer Cytomegalovirus microRNAs facilitate persistent virus infection in salivary glands.
description	Micro (mi)RNAs are small non-coding RNAs that regulate the expression of their targets' messenger RNAs through both translational inhibition and regulation of target RNA stability. Recently, a number of viruses, particularly of the herpesvirus family, have been shown to express their own miRNAs to control both viral and cellular transcripts. Although some targets of viral miRNAs are known, their function in a physiologically relevant infection remains to be elucidated. As such, no in vivo phenotype of a viral miRNA knock-out mutant has been described so far. Here, we report on the first functional phenotype of a miRNA knock-out virus in vivo. During subacute infection of a mutant mouse cytomegalovirus lacking two viral miRNAs, virus production is selectively reduced in salivary glands, an organ essential for virus persistence and horizontal transmission. This phenotype depends on several parameters including viral load and mouse genetic background, and is abolished by combined but not single depletion of natural killer (NK) and CD4+ T cells. Together, our results point towards a miRNA-based immunoevasion mechanism important for long-term virus persistence.
format	article
author	Lars Dölken Astrid Krmpotic Sheila Kothe Lee Tuddenham Mélanie Tanguy Lisa Marcinowski Zsolt Ruzsics Naama Elefant Yael Altuvia Hanah Margalit Ulrich H Koszinowski Stipan Jonjic Sébastien Pfeffer
author_facet	Lars Dölken Astrid Krmpotic Sheila Kothe Lee Tuddenham Mélanie Tanguy Lisa Marcinowski Zsolt Ruzsics Naama Elefant Yael Altuvia Hanah Margalit Ulrich H Koszinowski Stipan Jonjic Sébastien Pfeffer
author_sort	Lars Dölken
title	Cytomegalovirus microRNAs facilitate persistent virus infection in salivary glands.
title_short	Cytomegalovirus microRNAs facilitate persistent virus infection in salivary glands.
title_full	Cytomegalovirus microRNAs facilitate persistent virus infection in salivary glands.
title_fullStr	Cytomegalovirus microRNAs facilitate persistent virus infection in salivary glands.
title_full_unstemmed	Cytomegalovirus microRNAs facilitate persistent virus infection in salivary glands.
title_sort	cytomegalovirus micrornas facilitate persistent virus infection in salivary glands.
publisher	Public Library of Science (PLoS)
publishDate	2010
url	https://doaj.org/article/8cf511385a6746a9a38533828dd0fe01
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Cytomegalovirus microRNAs facilitate persistent virus infection in salivary glands.

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