Effective CRISPRa-mediated control of gene expression in bacteria must overcome strict target site requirements

The use of CRISPRa in bacteria lacks predictive rules for identifying effective gRNA target sites. Here the authors define features of bacterial promoters that impose stringent requirements on effective sites.

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Autores principales:	Jason Fontana, Chen Dong, Cholpisit Kiattisewee, Venkata P. Chavali, Benjamin I. Tickman, James M. Carothers, Jesse G. Zalatan
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2020
Materias:	Science Q
Acceso en línea:	https://doaj.org/article/8cfc1e2448d44cdbb571ab03ba5acc85
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spelling	oai:doaj.org-article:8cfc1e2448d44cdbb571ab03ba5acc852021-12-02T17:32:28ZEffective CRISPRa-mediated control of gene expression in bacteria must overcome strict target site requirements10.1038/s41467-020-15454-y2041-1723https://doaj.org/article/8cfc1e2448d44cdbb571ab03ba5acc852020-04-01T00:00:00Zhttps://doi.org/10.1038/s41467-020-15454-yhttps://doaj.org/toc/2041-1723The use of CRISPRa in bacteria lacks predictive rules for identifying effective gRNA target sites. Here the authors define features of bacterial promoters that impose stringent requirements on effective sites.Jason FontanaChen DongCholpisit KiattiseweeVenkata P. ChavaliBenjamin I. TickmanJames M. CarothersJesse G. ZalatanNature PortfolioarticleScienceQENNature Communications, Vol 11, Iss 1, Pp 1-11 (2020)
institution	DOAJ
collection	DOAJ
language	EN
topic	Science Q
spellingShingle	Science Q Jason Fontana Chen Dong Cholpisit Kiattisewee Venkata P. Chavali Benjamin I. Tickman James M. Carothers Jesse G. Zalatan Effective CRISPRa-mediated control of gene expression in bacteria must overcome strict target site requirements
description	The use of CRISPRa in bacteria lacks predictive rules for identifying effective gRNA target sites. Here the authors define features of bacterial promoters that impose stringent requirements on effective sites.
format	article
author	Jason Fontana Chen Dong Cholpisit Kiattisewee Venkata P. Chavali Benjamin I. Tickman James M. Carothers Jesse G. Zalatan
author_facet	Jason Fontana Chen Dong Cholpisit Kiattisewee Venkata P. Chavali Benjamin I. Tickman James M. Carothers Jesse G. Zalatan
author_sort	Jason Fontana
title	Effective CRISPRa-mediated control of gene expression in bacteria must overcome strict target site requirements
title_short	Effective CRISPRa-mediated control of gene expression in bacteria must overcome strict target site requirements
title_full	Effective CRISPRa-mediated control of gene expression in bacteria must overcome strict target site requirements
title_fullStr	Effective CRISPRa-mediated control of gene expression in bacteria must overcome strict target site requirements
title_full_unstemmed	Effective CRISPRa-mediated control of gene expression in bacteria must overcome strict target site requirements
title_sort	effective crispra-mediated control of gene expression in bacteria must overcome strict target site requirements
publisher	Nature Portfolio
publishDate	2020
url	https://doaj.org/article/8cfc1e2448d44cdbb571ab03ba5acc85
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Effective CRISPRa-mediated control of gene expression in bacteria must overcome strict target site requirements

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