Development of an ObLiGaRe Doxycycline Inducible Cas9 system for pre-clinical cancer drug discovery
CRISPR/Cas9 technology has revolutionised the ability of scientists to make genetically modified cells and animal models. Here, the authors make a Tet-On genetically modified mouse using the Streptococcus pyogenes Cas9 and demonstrate that it can be used for generation of mice with lung cancer.
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Nature Portfolio
2020
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oai:doaj.org-article:8d05a4aa843b419591409ca746b331db2021-12-02T18:51:23ZDevelopment of an ObLiGaRe Doxycycline Inducible Cas9 system for pre-clinical cancer drug discovery10.1038/s41467-020-18548-92041-1723https://doaj.org/article/8d05a4aa843b419591409ca746b331db2020-09-01T00:00:00Zhttps://doi.org/10.1038/s41467-020-18548-9https://doaj.org/toc/2041-1723CRISPR/Cas9 technology has revolutionised the ability of scientists to make genetically modified cells and animal models. Here, the authors make a Tet-On genetically modified mouse using the Streptococcus pyogenes Cas9 and demonstrate that it can be used for generation of mice with lung cancer.Anders LundinMichelle J. PorrittHimjyot JaiswalFrank SeeligerCamilla JohanssonAbdel Wahad BidarLukas BadertscherSandra WimbergerEmma J. DaviesElizabeth HardakerCarla P. MartinsEmily JamesTherese AdmyreAmir Taheri-GhahfarokhiJenna BradleyAnna SchantzBabak AlaeimahabadiMaryam ClausenXiufeng XuLorenz M. MayrRoberto NitschMohammad Bohlooly-YSimon T. BarryMarcello MarescaNature PortfolioarticleScienceQENNature Communications, Vol 11, Iss 1, Pp 1-16 (2020) |
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Science Q Anders Lundin Michelle J. Porritt Himjyot Jaiswal Frank Seeliger Camilla Johansson Abdel Wahad Bidar Lukas Badertscher Sandra Wimberger Emma J. Davies Elizabeth Hardaker Carla P. Martins Emily James Therese Admyre Amir Taheri-Ghahfarokhi Jenna Bradley Anna Schantz Babak Alaeimahabadi Maryam Clausen Xiufeng Xu Lorenz M. Mayr Roberto Nitsch Mohammad Bohlooly-Y Simon T. Barry Marcello Maresca Development of an ObLiGaRe Doxycycline Inducible Cas9 system for pre-clinical cancer drug discovery |
| description |
CRISPR/Cas9 technology has revolutionised the ability of scientists to make genetically modified cells and animal models. Here, the authors make a Tet-On genetically modified mouse using the Streptococcus pyogenes Cas9 and demonstrate that it can be used for generation of mice with lung cancer. |
| format |
article |
| author |
Anders Lundin Michelle J. Porritt Himjyot Jaiswal Frank Seeliger Camilla Johansson Abdel Wahad Bidar Lukas Badertscher Sandra Wimberger Emma J. Davies Elizabeth Hardaker Carla P. Martins Emily James Therese Admyre Amir Taheri-Ghahfarokhi Jenna Bradley Anna Schantz Babak Alaeimahabadi Maryam Clausen Xiufeng Xu Lorenz M. Mayr Roberto Nitsch Mohammad Bohlooly-Y Simon T. Barry Marcello Maresca |
| author_facet |
Anders Lundin Michelle J. Porritt Himjyot Jaiswal Frank Seeliger Camilla Johansson Abdel Wahad Bidar Lukas Badertscher Sandra Wimberger Emma J. Davies Elizabeth Hardaker Carla P. Martins Emily James Therese Admyre Amir Taheri-Ghahfarokhi Jenna Bradley Anna Schantz Babak Alaeimahabadi Maryam Clausen Xiufeng Xu Lorenz M. Mayr Roberto Nitsch Mohammad Bohlooly-Y Simon T. Barry Marcello Maresca |
| author_sort |
Anders Lundin |
| title |
Development of an ObLiGaRe Doxycycline Inducible Cas9 system for pre-clinical cancer drug discovery |
| title_short |
Development of an ObLiGaRe Doxycycline Inducible Cas9 system for pre-clinical cancer drug discovery |
| title_full |
Development of an ObLiGaRe Doxycycline Inducible Cas9 system for pre-clinical cancer drug discovery |
| title_fullStr |
Development of an ObLiGaRe Doxycycline Inducible Cas9 system for pre-clinical cancer drug discovery |
| title_full_unstemmed |
Development of an ObLiGaRe Doxycycline Inducible Cas9 system for pre-clinical cancer drug discovery |
| title_sort |
development of an obligare doxycycline inducible cas9 system for pre-clinical cancer drug discovery |
| publisher |
Nature Portfolio |
| publishDate |
2020 |
| url |
https://doaj.org/article/8d05a4aa843b419591409ca746b331db |
| work_keys_str_mv |
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