Rapid construction of metabolite biosensors using domain-insertion profiling

In the construction of single fluorescent protein biosensors, selection of the insertion point of a fluorescent protein into a ligand-binding domain is a rate-limiting step. Here, the authors develop an unbiased, high-throughput approach, called domain insertion profiling with DNA sequencing (DIP-se...

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Autores principales:	Dana C. Nadler, Stacy-Anne Morgan, Avi Flamholz, Kaitlyn E. Kortright, David F. Savage
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2016
Materias:	Science Q
Acceso en línea:	https://doaj.org/article/8d05fc327b244948b82af826e79987c9
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id	oai:doaj.org-article:8d05fc327b244948b82af826e79987c9
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spelling	oai:doaj.org-article:8d05fc327b244948b82af826e79987c92021-12-02T16:49:58ZRapid construction of metabolite biosensors using domain-insertion profiling10.1038/ncomms122662041-1723https://doaj.org/article/8d05fc327b244948b82af826e79987c92016-07-01T00:00:00Zhttps://doi.org/10.1038/ncomms12266https://doaj.org/toc/2041-1723In the construction of single fluorescent protein biosensors, selection of the insertion point of a fluorescent protein into a ligand-binding domain is a rate-limiting step. Here, the authors develop an unbiased, high-throughput approach, called domain insertion profiling with DNA sequencing (DIP-seq), to generate a novel trehalose biosensor.Dana C. NadlerStacy-Anne MorganAvi FlamholzKaitlyn E. KortrightDavid F. SavageNature PortfolioarticleScienceQENNature Communications, Vol 7, Iss 1, Pp 1-11 (2016)
institution	DOAJ
collection	DOAJ
language	EN
topic	Science Q
spellingShingle	Science Q Dana C. Nadler Stacy-Anne Morgan Avi Flamholz Kaitlyn E. Kortright David F. Savage Rapid construction of metabolite biosensors using domain-insertion profiling
description	In the construction of single fluorescent protein biosensors, selection of the insertion point of a fluorescent protein into a ligand-binding domain is a rate-limiting step. Here, the authors develop an unbiased, high-throughput approach, called domain insertion profiling with DNA sequencing (DIP-seq), to generate a novel trehalose biosensor.
format	article
author	Dana C. Nadler Stacy-Anne Morgan Avi Flamholz Kaitlyn E. Kortright David F. Savage
author_facet	Dana C. Nadler Stacy-Anne Morgan Avi Flamholz Kaitlyn E. Kortright David F. Savage
author_sort	Dana C. Nadler
title	Rapid construction of metabolite biosensors using domain-insertion profiling
title_short	Rapid construction of metabolite biosensors using domain-insertion profiling
title_full	Rapid construction of metabolite biosensors using domain-insertion profiling
title_fullStr	Rapid construction of metabolite biosensors using domain-insertion profiling
title_full_unstemmed	Rapid construction of metabolite biosensors using domain-insertion profiling
title_sort	rapid construction of metabolite biosensors using domain-insertion profiling
publisher	Nature Portfolio
publishDate	2016
url	https://doaj.org/article/8d05fc327b244948b82af826e79987c9
work_keys_str_mv	AT danacnadler rapidconstructionofmetabolitebiosensorsusingdomaininsertionprofiling AT stacyannemorgan rapidconstructionofmetabolitebiosensorsusingdomaininsertionprofiling AT aviflamholz rapidconstructionofmetabolitebiosensorsusingdomaininsertionprofiling AT kaitlynekortright rapidconstructionofmetabolitebiosensorsusingdomaininsertionprofiling AT davidfsavage rapidconstructionofmetabolitebiosensorsusingdomaininsertionprofiling
_version_	1718383171076096000

Rapid construction of metabolite biosensors using domain-insertion profiling

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